Banxia Xiexin decoction, a traditional Chinese medicine, alleviates colon cancer in nude mice

Abstract

Backgrounds: Banxia Xiexin decoction (BXD) is widely used in the treatment of acute and chronic gastritis, peptic ulcer, dyspepsia, gastrointestinal dysfunction, chronic hepatitis, oral ulcer and other diseases, but there are few reports on its treatment of colon cancer. The current study was designed to investigate the effect of BXD on colon cancer and its possible molecular mechanisms.Methods: Fifty SPF BALB/c nude mice were selected to establish an animal model of colon cancer bearing nude mice. Establishment of nude mice intestinal cancer model by subcutaneous injection of intestinal cancer cells. Serum superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by commercial kits. Pro-inflammatory cytokines in serum were detected. Western blotting was used to demonstrate the expression levels of related apoptosis proteins, inflammation and oxidative stress proteins.Results: Our results showed that BXD could decrease SOD and increased MDA in nude mice bearing tumors. BXD increased pro-inflammatory cytokines in serum in nude mice bearing tumors. Western blotting revealed that the protein expressions of Bax, Caspase-3, Caspase-9 were increased by different concentrations of BXD, while Bcl-2 was decreased. BXD also decreased Nrf-2 and HO-1, and increased the levels of MAPK/NF-κB pathway in tumor tissue.Conclusions: BXD has an obvious tumor inhibiting effect on SW 480 colon cancer transplanted tumor in nude mice via apoptotic pathway and MAPK/NF-κB pathway.

Therapeutic Emergence of Rhein as a Potential Anticancer Drug: A Review of Its Molecular Targets and Anticancer Properties

“…The Banxia Xiexin Decoction (BXD), one of its active components being rhein, is prescribed to ease various inflammatory disorders like gastritis and upper airway inflammation [78]. The BXD was also effective in curing colon cancer in animals [79].…”

“…Many biological properties of rhein have been studied, such as curing inflammatory disorders like upper airway inflammation, asthma, gastritis, and fatty liver and protecting against cerebral ischemic injury, diabetic nephropathy, etc. [42,78,79,91,92]. Several studies have shown that rhein is an effective antidiabetic, antiosteoarthritic and anticathartic agent [39,40,44,45,60].…”

“…Although rhein showed significant cytotoxicity in colon cancer cell line COLO 320 DM, it was found to be safe and did not cause DNA damage to normal colon cells, thus exhibiting selective toxicity [69]. In addition, recent studies on animal models of colon cancer have shown possible involvement of rhein in alleviating tumor proliferation via the activation of MAPK/NF-κB pathways [79].…”

β‑asarone suppresses HCT116 colon cancer cell proliferation and liver metastasis in part by activating the innate immune system

“…Conventionally in China, 5-fluorouracil (5-Fu) alone or in combination with other anticancer agents (such as irinotecan or oxaliplatin) has been frequently administered and has been shown to improve the survival rate of patients with partial colon cancer ( 5 , 6 ). In addition, the anticarcinogenic effect of several traditional Chinese medicines has also been emphasized in colon cancer ( 7 , 8 ). The authors' previous study demonstrated that modified Si-Jun-Zi Decoction (SJZ), a Chinese medicinal formula, could inhibit colon cancer liver metastasis by increasing the content of macrophage cells ( 9 ).…”

Effects of Xuefu Zhuyu Decoction on Cell Migration and Ocular Tumor Invasion inDrosophila

“…Traditional Chinese Medicine (TCM), with ancient application history in China or other regions of Asia, has gained considerable importance in defending against cancer during the last two decades [13][14][15]. Blood stasis, characterized by blood flow retardation and blood stagnation [16][17][18], is an essential factor for tumor metastasis during tumorigenesis in the TCM basic theory system [19,20].…”