Banking of human ovarian tissue potentially contaminated by cancer cells: experimental model for study of cryo-stability of these cells

Isachenko, Vladimir; Du, Xinxin; Isachenko, Evgenia; Todorov, Plamen; Mallmann, Peter; Щербак, О. В.; Rahimi, Gohar

doi:10.1007/s10561-019-09796-1

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…For short, in our experiments from cells of ZR-75-1 and MDA-MB-231 lines it was formed monolayer [ 15 ]. With aim to manipulate with a model of ovarian pieces, it was formed compacted fragments [ [14] , [15] , [22] ]. Then cancer cells were frozen and thawed by protocol of the ovarian tissue cryopreservation [ [23] , [24] , [25] , [26] , 28 , 29 ].…”

Section: Cancer Cells: Increasing Of Viability After Cryopreservationmentioning

confidence: 99%

High cryo-resistance of SARS-CoV-2 virus: Increased risk of re-contamination at transplantation of cryopreserved ovarian tissue after COVID-19 pandemic

Isachenko

Mallmann

et al. 2021

Cryobiology

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Cryopreservation and re-transplantation of ovarian tissue after anticancer treatment is important medical technology. Today, during a pandemic, the risk of contamination of transplanted cells with SARS-CoV-2 virus is extremely high. Data about cryo-resistance (virulence and/or infectivity) of SARS-CoV-2 are limited. Analysis and systematization of literature data allow us to draw the following conclusions: 1) The cytoplasmic membrane of somatic cell, like envelope of corona viruses, consists of lipid bilayer and this membrane, like envelope of corona virus, contains membrane proteins. Thus, we can consider the cytoplasmic membrane of an ordinary somatic cell as a model of the envelope membrane of SARS-CoV-2. It is expected that the response of the virus to cryopreservation is similar to that of a somatic cell. SARS-CoV-2 is more poor-water and more protein-rich than somatic cell, and this virus is much more cryo-resistant. 2) The exposure of somatic cells at low positive temperatures increases a viability of these cells. The safety of the virus is also in direct proportion to the decrease in temperature: the positive effect of low temperatures on SARS-CoV-2 virus has been experimentally proven. 3) Resistance of SARS-CoV-2 to cryoprotectant-free cryopreservation is extremely high. The high viability rate of SARS-CoV-2 after freezing-drying confirms its high cryo-resistance. 4) The risk of SARS-CoV-2 infection after transplantation of cryopreserved ovarian tissues that have been contaminated with this virus, increases significantly. Our own experimental data on the increase in the viability of cancer cells after cryopreservation allow us to formulate a hypothesis about increasing of viability (virulence and/or infectivity) of SARS-CoV-2 virus after cryopreservation.

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Section: Cancer Cells: Increasing Of Viability After Cryopreservationmentioning

confidence: 99%

High cryo-resistance of SARS-CoV-2 virus: Increased risk of re-contamination at transplantation of cryopreserved ovarian tissue after COVID-19 pandemic

Isachenko

Mallmann

et al. 2021

Cryobiology

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“…With the continuous development and progress of vitrification technology, more and more researchers apply this technology to the study of ovarian tissue cryopreservation [11]. Isachenko, V. et al [12] used nitrifications to freeze human ovarian tissue. After resuscitation, 86% of oocytes showed normal morphology.…”

Section: Rapid Freezingmentioning

confidence: 99%

Ovarian tissue freezing and activation after thawing: an update

Li-fan

2021

Middle East Fertil Soc J

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Background With the growth of women’s age, ovarian failure can be caused by various factors. For the women who need chemotherapy because of cancer factors, the preservation of fertility is more urgent. The treatment of cancer is also a process in which all tissues and organs of the body are severely damaged, especially in the reproductive system. Main body As a new fertility preservation technology, autologous ovarian tissue cryopreservation and transplantation is developing rapidly and showing great potentiality in preserving ovarian endocrine function of young cervical cancer patients. Vitrification and slow freezing are two common techniques applied for ovarian tissue cryopreservation. Thus, cryopreserved/thawed ovarian tissue and transplantation act as an important method to preserve ovarian function during radiotherapy and chemotherapy, and ovarian cryopreservation by vitrification is a very effective and extensively used method to cryopreserve ovaries. The morphology of oocytes and granulosa cells and the structure of organelles were observed under the microscope of histology; the hormone content in the stratified culture medium of granulosa cells with the diameter of follicle was used to evaluate the development potential of ovarian tissue, and finally the ovarian tissue stimulation was determined by the technique of ovarian tissue transplantation. Conclusions Although there are some limitations, the team members still carry out this review to provide some references and suggestions for clinical decision-making and further clinical research.

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Overall Approach to Fertility Preservation and Indications for Ovarian Tissue Harvesting and Cryopreservation

Turan

Türkgeldi²,

Öktem³

2022

Principles and Practice of Ovarian Tissue Cryopreservation and Transplantation

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Banking of human ovarian tissue potentially contaminated by cancer cells: experimental model for study of cryo-stability of these cells

Cited by 3 publications

References 17 publications

High cryo-resistance of SARS-CoV-2 virus: Increased risk of re-contamination at transplantation of cryopreserved ovarian tissue after COVID-19 pandemic

High cryo-resistance of SARS-CoV-2 virus: Increased risk of re-contamination at transplantation of cryopreserved ovarian tissue after COVID-19 pandemic

Ovarian tissue freezing and activation after thawing: an update

Overall Approach to Fertility Preservation and Indications for Ovarian Tissue Harvesting and Cryopreservation

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