Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice

Oh, You-Chang; Jeong, Yun Hee; Pak, Malk Eun; Go, Younghoon

doi:10.3390/nu12072019

Cited by 10 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Systemic exposure to the bacterial endotoxin, LPS, has been shown to elicit cognitive dysfunction in rodents [26] which is believed to have neuroinflammatory causes [27]. In fact, our results could thus indicate another possible mechanism to LPS-induced cognitive impairment-namely a decrease in CSF movement.…”

Section: Discussionmentioning

confidence: 63%

Acute systemic LPS-exposure impairs perivascular CSF distribution in mice

Manouchehrian

Ramos

Bachiller

et al. 2021

J Neuroinflammation

View full text Add to dashboard Cite

Background The exchange of cerebrospinal (CSF) and interstitial fluid is believed to be vital for waste clearance in the brain. The sleep-dependent glymphatic system, which is comprised of perivascular flow of CSF and is largely dependent on arterial pulsatility and astrocytic aquaporin-4 (AQP4) expression, facilitates much of this brain clearance. During the last decade, several observations have indicated that impaired glymphatic function goes hand in hand with neurodegenerative diseases. Since pathologies of the brain carry inflammatory components, we wanted to know how acute inflammation, e.g., with lipopolysaccharide (LPS) injections, would affect the glymphatic system. In this study, we aim to measure the effect of LPS on perivascular CSF distribution as a measure of glymphatic function. Methods Three hours after injection of LPS (1 mg/kg i.p.), C57bl/6 mice were (1) imaged for two CSF tracers, injected into cisterna magna, (2) transcardially perfused with buffer, or (3) used for physiological readouts. Tracer flow was imaged using a low magnification microscope on fixed brains, as well as using vibratome-cut slices for measuring tracer penetration in the brain. Cytokines, glial, and BBB-permeability markers were measured with ELISAs, Western blots, and immunohistochemistry. Cerebral blood flow was approximated using laser Doppler flowmetry, respiration and heart rate with a surgical monitor, and AQP4-polarization was quantified using confocal microscopy of immunolabeled brain sections. Results LPS-injections significantly lowered perivascular CSF tracer flow and penetration into the parenchyma. No differences in AQP4 polarization, cytokines, astroglial and BBB markers, cerebral blood flow, or respiration were detected in LPS-injected mice, although LPS did elevate cortical Iba1+ area and heart rate. Conclusions This study reports another physiological response after acute exposure to the bacterial endotoxin LPS, namely the statistically significant decrease in perivascular distribution of CSF. These observations may benefit our understanding of the role of systemic inflammation in brain clearance.

show abstract

Section: Discussionmentioning

confidence: 63%

Acute systemic LPS-exposure impairs perivascular CSF distribution in mice

Manouchehrian

Ramos

Bachiller

et al. 2021

J Neuroinflammation

View full text Add to dashboard Cite

show abstract

“… 29 , 30 Moreover, BHS prevented lipopolysaccharide-induced cognitive impairment and neuroinflammation in mice. 31 The herbal medicine formulae used in this observational study demonstrated potential effects on cognitive impairment in previous animal and human studies.…”

Section: Discussionmentioning

confidence: 66%

Herbal Medicine for Patients with Cognitive Impairment: An Observational Study

Choi

Kim

Lee

et al. 2021

NDT

Self Cite

View full text Add to dashboard Cite

Purpose:The potential effects of herbal medicine for patients with cognitive disorders have been reported in various human and animal studies. This study aimed to explore the effect of herbal medicine treatment according to the Korean Medicine (KM) pattern identification for patients with mild cognitive impairment and early dementia. Patients and Methods: Twenty patients with mild cognitive impairment or mild dementia who planned to receive herbal medicine treatment were enrolled. Herbal formulae were prescribed based on the KM pattern for 12-24 weeks. Seoul Neuropsychological Screening Battery II (SNSB-II) and Montreal Cognitive Assessment (MoCA) were assessed at the baseline, after 12 weeks, and after 24 weeks (Trial registration: cris.nih.go.kr, KCT0004799). Results: Herbal medicine products, including Yukmijihwang-tang, Samhwangsasim-tang, Palmul-tang, Banhasasim-tang, and Yukgunja-tang, were prescribed to the patients. Among the SNSB-II five cognitive function domains, the T scores for language, visuospatial function, memory, and frontal/executive function increased over time. The MoCA score also improved following the treatment (mean difference 4.23 [95% CI: 2.60, 5.86], p < 0.0001 at 12-week follow-up compared to the baseline). Considering the KM pattern scores, phlegm-dampness and fire-heat scores tended to improve after the treatment. No serious adverse events related to the intervention were reported. Conclusion:The potential effect of herbal medicine formulae products on improving cognitive functions in patients with cognitive impairment was observed. Further research is needed to objectify the KM pattern identification process and evaluate the KM patternrelated signs and symptoms.

show abstract

“…BHS is used for treating dyspepsia as a traditional decoction [ 20 ]. Our previous studies showed that BHS treatment attenuates neuroinflammation and cognitive dysfunction [ 22 ]. However, the effects of BHS on oxidative stress-induced neuronal damage remain largely unknown.…”

Section: Discussionmentioning

confidence: 99%

“…To test spatial memory, we performed the Morris water maze (MWM) test after administration of the drug, as described previously [ 22 ]. Briefly, we trained mice in a 1.2 m diameter pool filled with white water to reach a hidden platform (10 cm diameter) for 6 days.…”

Section: Methodsmentioning

confidence: 99%

“…BHS is currently prescribed for the treatment of functional dyspepsia [ 19 , 20 ] and has an antiapoptotic effect on chronic acid reflux esophagitis [ 21 ]. We recently demonstrated that BHS suppresses neuroinflammation in lipopolysaccharide-induced cognitive impairment [ 22 ]; however, the neuroprotective effect of BHS under oxidative stress conditions or its mechanisms are not well understood. Therefore, we hypothesized that BHS could be an antioxidant candidate to improve cognition in neuropathological conditions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Banhasasim‐Tang Ameliorates Spatial Memory by Suppressing Oxidative Stress through Regulation of ERK/p38 Signaling in Hippocampus of Mice

Pak

Park

et al. 2021

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

Since ancient times, Banhasasim-tang (BHS) has been used to treat functional dyspepsia in East Asia. Here, we aimed to determine the protective action of BHS on hippocampal neurons against oxidative stress. We investigated the functional effect of BHS on a scopolamine-induced mouse model, and molecular analysis was performed in glutamate-induced HT22 cells. We observed that BHS administration ameliorated memory dysfunction in scopolamine-treated mice. BHS administration also increased neuronal survival and acetylcholine activity and phosphorylation of extracellular signal-regulated kinase (ERK) and cAMP response element-binding protein (CREB) in the hippocampus of mice. In hippocampal cells, BHS treatment rescued glutamate-induced cytotoxicity, apoptosis, and oxidative stress. We observed an increase of HO-1 and a decrease of Nrf2 protein expression in glutamate-induced oxidative stress; however, the expression level of these proteins was significantly rescued by BHS treatment. BHS treatment also regulated phosphorylation of p38, p53, ERK, and CREB. Therefore, our data indicated that BHS may reduce oxidative stress through regulation of ERK-CREB and p38-p53 signaling in the hippocampus, resulting in decreased neuronal damage and improved memory in rodent models of neurodegenerative disease.

show abstract

Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice

Cited by 10 publications

References 36 publications

Acute systemic LPS-exposure impairs perivascular CSF distribution in mice

Acute systemic LPS-exposure impairs perivascular CSF distribution in mice

Herbal Medicine for Patients with Cognitive Impairment: An Observational Study

Banhasasim‐Tang Ameliorates Spatial Memory by Suppressing Oxidative Stress through Regulation of ERK/p38 Signaling in Hippocampus of Mice

Contact Info

Product

Resources

About