Bandenverbreiterung innerhalb und außerhalb einer flüssigkeits-chromatographischen Kolonne

Halász, I.; Gerlach, H. O.; Kroneisen, A.; Walkling, P.

doi:10.1007/bf00508916

Cited by 33 publications

(6 citation statements)

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“…One study (270) indicates an advantage when the column temperature is controlled, while another (397) finds little advantage in the use of positive and negative temperature programming. Band broadening in different types of packed columns (160) and in the detector and the detector-column joint (159) has been determined. The classical Van Deemter equation must be expanded for application to fast liquid chromatography (469).…”

Section: Column Adsorption and Partition Chromatographymentioning

confidence: 99%

Chromatography

Zweig¹,

Moore²,

Sherma³

1970

Anal. Chem.

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Section: Column Adsorption and Partition Chromatographymentioning

confidence: 99%

Chromatography

Zweig¹,

Moore²,

Sherma³

1970

Anal. Chem.

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“…A practical drawback of this method and the commercial product is the limitation to isocratic analysis, making the approach inadequate for mixtures of compounds that differ significantly in polarity and hydrophobicity where a gradient analysis is needed for obtaining acceptable retention times. Moreover, late eluting compounds may suffer from excessive peak broadening and decreasing compound detectability. , …”

mentioning

confidence: 99%

Stationary-Phase Optimized Selectivity Liquid Chromatography: Development of a Linear Gradient Prediction Algorithm

et al. 2010

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Stationary-phase optimized selectivity liquid chromatography (SOS-LC) is a tool in reversed-phase LC (RP-LC) to optimize the selectivity for a given separation by combining stationary phases in a multisegment column. The presently (commercially) available SOS-LC optimization procedure and algorithm are only applicable to isocratic analyses. Step gradient SOS-LC has been developed, but this is still not very elegant for the analysis of complex mixtures composed of components covering a broad hydrophobicity range. A linear gradient prediction algorithm has been developed allowing one to apply SOS-LC as a generic RP-LC optimization method. The algorithm allows operation in isocratic, stepwise, and linear gradient run modes. The features of SOS-LC in the linear gradient mode are demonstrated by means of a mixture of 13 steroids, whereby baseline separation is predicted and experimentally demonstrated.

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“…In the second arrangement a squeezed capillary (29) was connected between the sampling system and column. The original diameter of the stainless steel capillaries was 0.25 mm and their length varied between 8 cm and 15 cm.…”

mentioning

confidence: 99%