Band broadening in electrokinetic chromatography with micellar solutions and open-tubular capillaries

Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

“…Applying the same instrumentation as in capillary zone electrophoresis, in MECC uncharged compounds can be separated based on differences in their partitioning between two phases, just as in chromatographic techniques. Since the introduction of MECC by Terabe et al [1,2], several authors have paid attention to the fundamental characteristics of this separation method [3,4] and to the effect of different separation parameters on the migration behavior [5][6][7][8][9][10][11]. Also the theoret-the solute in the micellar and the aqueous phase, and ts, tEO F and tMC are the migration times of the solute, the electroosmotic flow (EOF) and the micelles, respectively.…”

Fundamentals of micellar electrokinetic chromatography (MEKC)

“…In 1981, Jorgenson and Lukas [3,4] demonstrated highly efficient electrophoresis separations by performing electrophoresis in narrow-bore capillaries filled with buffer, normally in the range from 25 to 100 m of internal diameter (i.d). As mentioned earlier, neutral analytes cannot be separated by simple electrophoresis experiment, the charged surfactants above their CMC were used by Terabe et al [5] in the CE running buffer. The micelle formed, allowed separation of uncharged molecules along with the charged ones based on hydrophobic affinity of the neutral molecules for the micelle.…”

Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins

“…Further efforts were devoted to aspects such as axial diffusion [23], Joule heat [24], interaction between the capillary wall and analytes [25 -27], EOF [28,29], and non-linearity effects [30,31]. The detailed descriptions can be found in the references and literatures there included.…”