Band-3 protein function in human erythrocytes: effect of oxygenation–deoxygenation

Galtieri, Antonio; Tellone, Ester; Romano, L.; Misiti, Francesco; Bellocco, Ersilia; Ficarra, Silvana; Russo, Annamaria; Rosa, Domenica Di; Castagnola, Massimo; Giardina, Bruno; Messana, Irene

doi:10.1016/s0005-2736(02)00454-6

Cited by 44 publications

(33 citation statements)

References 26 publications

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“…For instance, comparison of the kinetics of band 3-mediated Cl-transport in fish (carp, rainbow trout, eel and cod) and human erythrocytes did not reveal a significant difference between oxygenated and deoxygenated RBCs in the species studied [33]. Nor was O 2 -dependence of the anion exchanger revealed in crocodilian [34] and chicken [35] RBCs. On the other hand, there are recent reports that in normal human red cells, oxygenation stimulates the anion exchanger [35,36].…”

Section: +mentioning

confidence: 99%

Erythrocyte Signal Transduction Pathways, their Oxygenation Dependence and Functional Significance

Barvitenko

Adragna

Weber³

2005

Cell Physiol Biochem

135

104

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Erythrocytes play a key role in human and vertebrate metabolism. Tissue O₂ supply is regulated by both hemoglobin (Hb)-O₂ affinity and erythrocyte rheology, a key determinant of tissue perfusion. Oxygenation-deoxygenation transitions of Hb may lead to re-organization of the cytoskeleton and signalling pathways activation/deactivation in an O₂-dependent manner. Deoxygenated Hb binds to the cytoplasmic domain of the anion exchanger band 3, which is anchored to the cytoskeleton, and is considered a major mechanism underlying the oxygenation-dependence of several erythrocyte functions. This work discusses the multiple modes of Hb-cytoskeleton interactions. In addition, it reviews the effects of Mg²⁺, 2,3-diphosphoglycerate, NO, shear stress and Ca²⁺, all factors accompanying the oxygenation-deoxygenation cycle in circulating red cells. Due to the extensive literature on the subject, the data discussed here, pertain mainly to human erythrocytes whose O₂ affinity is modulated by 2,3-diphosphoglycerate, ectothermic vertebrate erythrocytes that use ATP, and to bird erythrocytes that use inositol pentaphosphate.

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Section: +mentioning

confidence: 99%

Erythrocyte Signal Transduction Pathways, their Oxygenation Dependence and Functional Significance

Barvitenko

Adragna

Weber³

2005

Cell Physiol Biochem

135

104

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“…Our previous work highlighted that in adult human erythrocytes sulphate transport across the membrane is modulated by the pressure of oxygen and therefore by the oxygen-linked transition of Hb [17]. The same study performed on pig erythrocytes is shown in Figure 1 and Figure 2.…”

Section: Resultsmentioning

confidence: 61%

“…We have already discussed the involvement of the conformational transition (T-R) of Hb on the different activities of human B3 in HOS and LOS state. We associated this finding with the structural hindrance caused by the gradual increase of Hb bound to cdb3 that would take place on passing from HOS to LOS [17] [26]. In the case of pig RBCs (Figure 2), the stronger involvement of Hb in the modulation of B3 could be affected by the contribution of free Cl − which is lower in the LOS state as consequence of the binding to Hb.…”

Section: Discussionmentioning

confidence: 96%

“…All these considerations could be closely associated with the low sodium transport (and number of sodium pumps) which has been observed in pig in comparison to human RBCs [35]. Nevertheless the increase of Cl − activity is not sufficient to justify the high anion exchange observed in HOS erythrocyte [17]. Another important event that occurs during the transition HOS-LOS of the RBC is the change of the intracellular concentration of free 2,3-DPG.…”

Section: Discussionmentioning

confidence: 99%

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Metabolic Effects of Endogenous and Exogenous Heterotropic Hemoglobin Modulators on Anion Transport: The Case of Pig Erythrocytes

Tellone

Russo²,

Giardina³

et al. 2015

OALib

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This comparative study, focused on the anion kinetic of band 3 protein of pig erythrocytes, investigates in vitro the metabolic functionality of the red blood cells of this mammal which show particular characteristics of the cell membrane and significant stoichiometric variations of several cytosolic components. Pig red blood cells compared to human ones, are characterized by a higher anion flux strongly modulated by the conformational transition state (T-R) of hemoglobin; these findings are probably connected to the intracellular concentration of 2,3-disphosphoglycerate and to chloride activity. The experimental use, alone or in combination, of some exogenous heterotropic modulators of hemoglobin as orthovanadate, gemfibrozil and resveratrol, shows characteristic modulations of the anion flux which are related to the cytosolic metabolites physiologically present in pig erythrocytes, to the metabolism and oxidative stress. Our findings highlight new features about the pig red blood cells functionality and contribute to extending the current understanding of their life cycle, providing opportunities for new investigations in the field of comparative physiology.

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“…AE1 is found in erythrocytes and the kidneys, AE2 is found in a wide range of tissues, and AE3 is found in the brain, retina and heart. All members of the anion exchanger family have two domains: an N-terminal cytoplasmic domain that contains binding sites for glycolytic enzymes and hemoglobin; and a C-terminal membrane domain [8,9]. The membrane domain is highly conserved.…”

Section: Introductionmentioning

confidence: 99%

Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

Gugliotta

Luca

Romano

et al. 2012

Cellular and Molecular Biology Letters

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Our study concerns the effects of exposure to lead chloride on the morphology, K + efflux, SO 4 -influx and GSH levels of the human erythrocyte. Blood was collected in heparinized tubes and washed three times. The cells were suspended at 3% hematocrit and incubated for 1 h at 25ºC in a medium containing increasing concentrations of lead chloride (0, 0.3, 0.5 and 1 µM). After incubation, the suspensions were centrifuged and the erythrocyte pellets were divided into three aliquots for testing. The results show: an increase in the permeability of erythrocytes treated with lead chloride with consequent damage and cellular death, especially in the presence of high concentrations; an increase in potassium ion efflux; alterations in the morphology and membrane structure of the red blood cells; and a decrease in sulphate uptake, due either to the oxidative effect of this compound on the band 3 protein, which loses its biological valence as a carrier of sulphate ions, or to a decrease in the ATP erythrocyte concentration. In conclusion, the exposure of erythrocytes to Pb

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Band-3 protein function in human erythrocytes: effect of oxygenation–deoxygenation

Cited by 44 publications

References 26 publications

Erythrocyte Signal Transduction Pathways, their Oxygenation Dependence and Functional Significance

Erythrocyte Signal Transduction Pathways, their Oxygenation Dependence and Functional Significance

Metabolic Effects of Endogenous and Exogenous Heterotropic Hemoglobin Modulators on Anion Transport: The Case of Pig Erythrocytes

Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

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