Banana Bunchy Top Viral Coat Protein (CP) Gene Expression Studies at Molecular Level in Hill Banana cv. Sirumalai (AAB)

Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia

“…However, there are some of potential problems in PCR assay for diagnostic purposes include false negatives due to reaction failure of bad quality DNA, contains high phenolic compounds so that even though the target viral DNA is present but still fail to amplify. False positives also may appear due to DNA contamination symptoms (Arumugam et al 2017). Pisang Madu and Moseng as the new collections with no visual morphological symptoms were confirm positively BBTV infected as detected by PCR (Figure 2, lane 9 and 10, respectively).…”

“…Some DNA-S BBTV CP primers available may amplified at full length or partial sequences. Previous studies by Abdulkader et al (2004), El-Dougdoug and El-Shamy (2011), and Hazaa et al 2006 to Egyptian banana isolates; Mahadev et al (2013), Shilpa et al (2016), Arumugam et al (2017) to Indian isolates, Aquino et al (2005) to Phillipines isolates, Niyongere et al (2015) to African isolates were produced expected amplicons approximately of ±500 bp (partial length). Whilst, some studies such as Yu et al (2012) to Chinese isolates, Amin et al (2008) to Pakistan isolates; Selvarajan et al (2010) to Indian isolates, Stainton et al (2015) to various isolates around the world, Wickramaarachchi et al 2016 to Sri Lankan isolates, Furuya et al (2004) and Chiaki et al (2015) to Indonesian isolates were using primers with expected amplicons of approximately ±1,000-1,100 bp (full length).…”

“…The PCR amplified products of BBTV CP gene particularly the full length amplicons should be cloned into plasmid vectors (such as pCRII, pCR2000, ptz57R/T, pTZBBTri4, pET 28a, etc.) prior to sequencing to get clean specific target sequences (Arumugam et al 2017;Burns et al 1995;Das et al 2007;Kumar et al 2017).…”

“…This study was aimed to confirms the BBTV infection of some symptomatic and symptomless banana accessions from Indonesia through PCR based detection using specific primers of banana bunchy top virus gene for coat protein (CP). The CP encoded by the DNA-S component of the BBTV has been used for virus detection because of its important role in viral particles packing and disease establishment and has been consistently associated with BBTV in Asian countries group (Arumugam et al 2017). In addition, the viral coat protein sequences also analyzed and aligned with those already reported in Genbank for phylogenetic analysis, particularly isolates from Asian countries.…”

Development and validation of allele specificRLKgene markers for banana bunchy top disease (BBTD) resistance in banana

“…In the Philippines, BBTD incidence has been slowly increasing for the past decades which greatly affected the livelihood of smallholder farmers as well as large-scale growers (Molina et al 2009). Controlling the spread of disease in bananas includes elimination of infected plants, use of virus-free planting materials, management of insect vector populations, and early detection of infected plants (Hong-Ji 2000; Bajet and Magnaye 2002; Dela Cueva et al 2010; Arumugam et al 2017). However, the use of BBTV-resistant banana cultivars remain to be the most effective and sustainable way of managing disease and minimizing yield loss in banana production (Jekayinoluwa et al 2020; Tripathi et al 2020).…”