Extensive efforts to further promoting the Anti-Bacteria and structural properties of thin films to reach reliability and possibility of commercialization, the chemical Tri-metal oxide component was verification as Anti-Bacteria factor in this paper. Pure and mixed thin films of magnesium oxide MgO was prepared by evaporation assisted laser Nedmyum - YAG pulse Nd: YAG laser system, MgO enhanced by adding Ti and Se, at (0.5, 1, 1.5 and 2%) by weight percentage. After that, calcination is done at 400 °C for 30 min. Structural and anti-bacterial growth inspections were performed. Experimental results showed that structural properties have improved significantly with the development of a MgO thin films with tri-metal oxide; Magnesium titanium oxide Mg2TiO4 and Magnesium selenate MgSeO4 phases. Moreover, there has been an enhancement in anti-bacteria properties, which makes these thin films more reliable for protection against bacteria.