Bamboo salt reduces allergic responses by modulating the caspase-1 activation in an OVA-induced allergic rhinitis mouse model

Kim, Kyu-Yeob; Nam, Sun‐Young; Shin, Tae‐Yong; Park, Kun-Young; Jeong, Hyun‐Ja; Kim, Hyung-Min

doi:10.1016/j.fct.2012.07.017

Cited by 26 publications

(16 citation statements)

References 37 publications

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“…Currently there are no reliable genetic mouse models of rhinitis or sinusitis. The most popular nongenetic mouse models of allergic sinonasal inflammation are based on modifications of previously well‐characterized lower airway models using various concentrations and sensitization protocols with ovalbumin, Aspergillus , or common allergens such as house dust mite …”

Section: Discussionmentioning

confidence: 99%

“…Last, the experimental techniques used to assess levels of sinonasal inflammation also vary greatly. Although some groups rely heavily on qualitative measures such as histology and the presence of inflammatory cells such as eosinophils and behavioral changes such as nasal rubbing and sneezing, others measure cytokine levels in sinonasal lavage fluid or sinonasal mucosal tissue . Clearly, there can be a high degree of variability in both the creation and characterization of the allergic sinonasal mouse model.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a novel high‐dose ovalbumin‐induced murine model of allergic sinonasal inflammation

Mendiola¹,

Tharakan²,

Chen³

et al. 2016

Int Forum Allergy Rhinol

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BACKGROUND Few efficacious topical therapies exist for chronic rhinosinusitis. The lack of a reproducible mouse model of CRS limits the pilot testing of potential novel anti-inflammatory therapies. Although the ovalbumin induced mouse model of sinonasal inflammation is commonly used, it is difficult to reproduce and can generate variable histologic results. In this study, we explore a variation of this model in different strains of mice and explore various inflammatory cytokines as reproducible molecular markers of inflammation. METHODS Allergic sinonasal inflammation was generated in BALB/c and C57BL/6 mice using intraperitoneal high dose injections of Ova followed by 10 days of high dose intranasal sensitization. Real-Time PCR for eotaxin, IL4, and IL13 were measured from sinonasal mucosa. We also pilot tested a known topical budesonide to characterize the anti-inflammatory response. Histological sections were analyzed for epithelial thickness and eosinophilia. RESULTS Both BALB/c and C57BL/6 mice consistently showed increases in Th2 cytokines after sensitization with high dose Ova (p<0.0001) when compared to controls. There were also significant increases in epithelial thickening in Ova sensitized mice and eosinophilia in both BALB/c and C57BL/6 strains.In addition, topical budesonide significantly reduced anti-inflammatory cytokines, eosinophilia, and epithelial thickness. CONCLUSION Our variation of the ovalbumin induced mouse model of sinonasal inflammation in both BALB/c and C57BL/6 mice provides an efficacious model for testing potential topical anti-inflammatory therapies for CRS. The utilization of sinonasal mucosal Th2 cytokines along with histologic markers provides a consistent and quantifiable marker of inflammation in assessing the efficacy of candidate drugs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of a novel high‐dose ovalbumin‐induced murine model of allergic sinonasal inflammation

Mendiola¹,

Tharakan²,

Chen³

et al. 2016

Int Forum Allergy Rhinol

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“…AR is a type I allergic disease induced by IgE-mediated inflammation and characterized by the release of HIS [30]. As summarized in Figure 3(b), the total IgE levels in rats in MFXD (7.6 g/kg) and positive groups were 2.23 and 2.13 U/mL, respectively, which were significantly reduced compared with that of 2.86 U/mL in the AR model group ( p < 0.01; p < 0.01).…”

Section: Resultsmentioning

confidence: 99%

Mahuang Fuzi Xixin Decoction Attenuates Th1 and Th2 Responses in the Treatment of Ovalbumin-Induced Allergic Inflammation in a Rat Model of Allergic Rhinitis

Ren

Tang

Chen

et al. 2017

Journal of Immunology Research

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Allergic rhinitis (AR) is one of the most common allergic diseases, which adversely affect patients' quality of life. Mahuang Fuzi Xixin decoction (MFXD) has been widely used to treat AR in clinics in Asian countries. This study investigated the effect and possible therapeutic mechanisms of MFXD in the treatment of AR. A Wistar rat model of ovalbumin- (OVA-) induced AR was established and then treated with three doses of MFXD; AR symptoms, serum total immunoglobulin E, histamine, histopathological features, and release and expression of factors related to type 1 helper T (Th1) and type 2 helper T (Th2) responses were analyzed. Our study demonstrated that MFXD has a good therapeutic effect on OVA-induced allergic inflammation in an AR rat model as manifested in reduced frequencies of sneezing and nasal scratching and in reduced serum levels of total IgE and HIS. In addition, MFXD regulates imbalance in Th1/Th2 cells caused by AR by simultaneously attenuating Th1 and Th2 responses, such as by reducing the serum levels of IFN-γ and IL-4 and mRNA expression levels of IFN-γ, IL-4, GATA-3, and STAT-6. This study provided valuable information on the immunoregulatory effect of MFXD for the treatment of AR in future clinical studies.

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“…Caspase‐1 specifically cleaves the inactive IL‐1β precursor and causes it to be secreted as a bioactive mature form, IL‐1β, which is a critical factor in relation to inflammation (Nam et al, ). Kim et al () reported that solar salt did not inhibit PMACI‐induced caspase‐1 activity despite the anti‐inflammatory effect of solar salt. In contrast, the present study shows that BS inhibits PMACI‐induced caspase‐1 activation.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory efficacy of Beauty-salt is mediated by the caspase-1/NF-κB/RIP2/MAP kinase pathway in mast cells

et al. 2017

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Beauty‐salt (BS, Mi‐salt) is a type of food additive mainly used in Korea. It is composed of solar salt and Impatiens balsamina L. extracts. Here, the present study reports the immunomodulatory efficacy and underlying mechanisms of BS and its active components, ferulic acid (FA) and magnesium (Mg), on the human mast cell line HMC‐1. BS significantly suppressed the production and mRNA expression of the proinflammatory cytokines, thymic stromal lymphopoietin (TSLP), interleukin (IL)‐1β, IL‐6, and IL‐8 in activated HMC‐1 cells without cytotoxicity. FA or Mg significantly decreased the production of TSLP and IL‐1β but not IL‐6 and IL‐8. In activated mast cells, the pathways of caspase‐1, nuclear factor (NF)‐κB, and receptor‐interacting protein 2 (RIP2) were blocked by treatment with BS, FA, or Mg. Furthermore, BS suppressed the activation of mitogen‐activated protein (MAP) kinases. The present findings provide new scientific evidence that BS has good immunomodulatory efficacy by down‐regulating caspase‐1/NF‐κB/RIP2/MAP kinase pathways. Practical applications Beauty‐salt (BS, Mi‐salt) is a type of food additive mainly used in Korea. BS inhibited the production of proinflammatory cytokines via the blockade of caspase‐1, NF‐κB, and MAPK pathways. Therefore, these results suggest that BS has immunomodulatory efficacy that can play a beneficial role in strategies to prevent and treat inflammatory disorders.

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Bamboo salt reduces allergic responses by modulating the caspase-1 activation in an OVA-induced allergic rhinitis mouse model

Cited by 26 publications

References 37 publications

Characterization of a novel high‐dose ovalbumin‐induced murine model of allergic sinonasal inflammation

Characterization of a novel high‐dose ovalbumin‐induced murine model of allergic sinonasal inflammation

Mahuang Fuzi Xixin Decoction Attenuates Th1 and Th2 Responses in the Treatment of Ovalbumin-Induced Allergic Inflammation in a Rat Model of Allergic Rhinitis

Immunomodulatory efficacy of Beauty-salt is mediated by the caspase-1/NF-κB/RIP2/MAP kinase pathway in mast cells

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