Bam and Otu can regulate stem cell fate by stabilizing cyclin A

Mathieu, Juliette; Huynh, Jean‐René

doi:10.1073/pnas.1706840114

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…The results showed that overexpression of CycA with CPT exposure could enhance the toxicity of CPT exposure, exhibiting a higher rate of GSC loss and more two-cell accumulation ( Figure 7 a). These findings were consistent with a previous study, where ectopic expression of Bam in GSCs could be enhanced by co-expression of CycA [ 36 ]. However overexpression of cycB ( Figure 7 b) or cycE RNAi ( Figure 7 c) did not display expanded undifferentiated cell phenotypes.…”

Section: Resultssupporting

confidence: 94%

“…These results suggested that changes in CycA level are sufficient to explain the loss of GSCs when expressing Bam ectopically. Consistent with the previous study, they found that ectopic expression of the stable form of CycA in germ cells caused GSC loss, which is similar to the phenotype resulting from ectopic expression of Bam in GSCs [ 36 , 48 ]. Ji et al convincingly showed that ectopic expression of Bam in GSC could be enhanced by co-expression of CycA, and suppressed by CycA reduction [ 24 ].…”

Section: Discussionsupporting

confidence: 88%

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Camptothecin Effectively Regulates Germline Differentiation through Bam–Cyclin A Axis in Drosophila melanogaster

Zhang

Sun

et al. 2023

IJMS

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Camptothecin (CPT), first isolated from Chinese tree Camptotheca acuminate, produces rapid and prolonged inhibition of DNA synthesis and induction of DNA damage by targeting topoisomerase I (top1), which is highly activated in cancer cells. CPT thus exhibits remarkable anticancer activities in various cancer types, and is a promising therapeutic agent for the treatment of cancers. However, it remains to be uncovered underlying its cytotoxicity toward germ cells. In this study we found that CPT, a cell cycle-specific anticancer agent, reduced fecundity and exhibited significant cytotoxicity toward GSCs and two-cell cysts. We showed that CPT induced GSC loss and retarded two-cell cysts differentiation in a niche- or apoptosis-independent manner. Instead, CPT induced ectopic expression of a differentiation factor, bag of marbles (Bam), and regulated the expression of cyclin A, which contributed to GSC loss. In addition, CPT compromised two-cell cysts differentiation by decreasing the expression of Bam and inducing cell arrest at G1/S phase via cyclin A, eventually resulting in two-cell accumulation. Collectively, this study demonstrates, for the first time in vivo, that the Bam–cyclin A axis is involved in CPT-mediated germline stem cell loss and two-cell cysts differentiation defects via inducing cell cycle arrest, which could provide information underlying toxicological effects of CPT in the productive system, and feature its potential to develop as a pharmacology-based germline stem cell regulation agent.

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Section: Resultssupporting

confidence: 94%

Section: Discussionsupporting

confidence: 88%

Camptothecin Effectively Regulates Germline Differentiation through Bam–Cyclin A Axis in Drosophila melanogaster

Zhang

Sun

et al. 2023

IJMS

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Dynamic regulation of ribosome levels and translation during development

Breznak

Kotb

Rangan

2023

Seminars in Cell & Developmental Biology

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A model of replicating coupled oscillators generates naturally occurring cell networks

Smart,

Shvartsman,

Nunley

2023

Development

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When a founder cell and its progeny divide with incomplete cytokinesis, a network forms in which each intercellular bridge corresponds to a past mitotic event. Such networks are required for gamete production in many animals, and different species have evolved diverse final network topologies. While mechanisms regulating network assembly have been identified in particular organisms, we lack a quantitative framework to understand network assembly and inter-species variability. Motivated by cell networks responsible for oocyte production in invertebrates, where the final topology is typically invariant within each species, we devise a mathematical model for generating cell networks: each node is an oscillator, and after a full cycle, the node produces a daughter to which it remains connected. These cell cycle oscillations are transient and coupled via diffusion over the network's edges. By variation of three biologically motivated parameters, our model generates nearly all such networks currently reported across invertebrates. Furthermore, small parameter variations can rationalize cases of within-species variation. Because cell networks outside of the ovary often form less deterministically, we propose model generalizations to account for sources of stochasticity.

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Bam and Otu can regulate stem cell fate by stabilizing cyclin A

Cited by 3 publications

References 23 publications

Camptothecin Effectively Regulates Germline Differentiation through Bam–Cyclin A Axis in Drosophila melanogaster

Camptothecin Effectively Regulates Germline Differentiation through Bam–Cyclin A Axis in Drosophila melanogaster

Dynamic regulation of ribosome levels and translation during development

A model of replicating coupled oscillators generates naturally occurring cell networks

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