Balnase, a New Dimer-Forming Ribonuclease from Bacillus altitudinis

Dudkina, Elena; Ulyanova, Vera; Ilinskaya, О. N.

doi:10.1007/s12668-016-0305-y

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…Investigation of the structural organization of the RNases isolated by us – binase, balnase, and balifase – has revealed that all of them dimerize in vivo and are natural dimers [ 41 , 44 , 45 ]. Probably, the formation of RNase dimers is one of the key processes necessary for the enzymes to perform their functions and manifest their biological properties.…”

Section: Secreted Ribonucleases Of Bacillimentioning

confidence: 99%

“…Some binase dimers are highly stable, apparently due to the exchange of N- or C-terminal regions, and do not dissociate under denaturing conditions; others are incapable of exchanging domains between monomers (swapping interactions), which leads to the dissociation of these dimers into monomers during electrophoresis under denaturing conditions [ 44 ]. Balnase and balifase constitute only the second type of dimers [ 22 , 41 , 45 ].…”

Section: Secreted Ribonucleases Of Bacillimentioning

confidence: 99%

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Supramolecular organization as a factor of ribonuclease cytotoxicity

2020

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One of the approaches used to eliminate tumor cells is directed destruction/modification of their RNA molecules. In this regard, ribonucleases (RNases) possess a therapeutic potential that remains largely unexplored. It is believed that the biological effects of secreted RNases, namely their antitumor and antiviral properties, derive from their catalytic activity. However, a number of recent studies have challenged the notion that the activity of RNases in the manifestation of selective cytotoxicity towards cancer cells is exclusively an enzymatic one. In this review, we have analyzed available data on the cytotoxic effects of secreted RNases, which are not associated with their catalytic activity, and we have provided evidence that the most important factor in the selective apoptosis-inducing action of RNases is the structural organization of these enzymes, which determines how they interact with cell components. The new idea on the preponderant role of non-catalytic interactions between RNases and cancer cells in the manifestation of selective cytotoxicity will contribute to the development of antitumor RNase-based drugs.

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Section: Secreted Ribonucleases Of Bacillimentioning

confidence: 99%