Ball milled glyco-graphene oxide conjugates markedly disrupted <i>Pseudomonas aeruginosa</i> biofilms

Tricomi, Jacopo; Cacaci, Margherita; Biagiotti, Giacomo; Caselli, Lucrezia; Niccoli, Lorenzo; Torelli, Riccardo; Gabbani, Alessio; Vito, Maura Di; Pineider, Francesco; Sanguinetti, Maurizio; Menna, Enzo; Lelli, Moreno; Berti, Debora; Cicchi, Stefano; Bugli, Francesca; Richichi, Barbara

doi:10.1039/d2nr02027k

Cited by 5 publications

(5 citation statements)

References 68 publications

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“…However, previous reports have shown that the size of nanomaterials prepared through ball-milling was relatively large. [174][175][176] Very few reports have produced quantum-sized materials from layered materials through ball-milling. Yang et al prepared CQDs from activated carbon by high-energy ball-milling (HEBM).…”

Section: Mechanical Exfoliationmentioning

confidence: 99%

A Review of Top‐Down Strategies for the Production of Quantum‐Sized Materials

Chen,

Zhao,

Zhou

et al. 2023

Small Science

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Nanoscience and technology have made significant achievements in the past few decades. Quantum‐sized materials, as a key component of nanomaterials, have attracted increasing interest due to their unique structures and extremely reduced sizes. Such fascinating materials have been widely applied in various fields because of their strong quantum confinement and remarkable surface/edge effects. Production methods have an important impact on the properties of quantum‐sized materials. Considering that many previous reviews have reported the synthesis of quantum‐sized materials by bottom‐up methods, this review will focus on the top‐down methods. The advantages and disadvantages of each strategy are analyzed. At the end, the perspectives and challenges toward the future development of quantum‐sized materials are discussed.

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Section: Mechanical Exfoliationmentioning

confidence: 99%

A Review of Top‐Down Strategies for the Production of Quantum‐Sized Materials

Chen,

Zhao,

Zhou

et al. 2023

Small Science

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“…In contrast to graphene, GO is relatively easy to functionalize, owing to the varied functionalities, mainly epoxide and hydroxyl groups, on its surface. [11][12][13] Importantly, GO is rapidly degraded by human peroxidases avoiding longterm side effects that might be associated with bioaccumulation. [14] In the field of drug delivery, size and functionalization play an important role on how cells and tissues interact with materials.…”

Section: Introductionmentioning

confidence: 99%

“…Such a characteristic is key in terms of forming stable GO colloids in water and/or in polar solvents. In contrast to graphene, GO is relatively easy to functionalize, owing to the varied functionalities, mainly epoxide and hydroxyl groups, on its surface [11–13] . Importantly, GO is rapidly degraded by human peroxidases avoiding long‐term side effects that might be associated with bioaccumulation [14] …”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide‐BODIPY Conjugates as Highly Fluorescent Materials

Reina

Beneventi

Kaur

et al. 2023

Chemistry A European J

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Covalent functionalization of graphene oxide (GO) with boron dipyrromethenes (BODIPYs) was achieved through a facile synthesis, affording two different GO-BODIPY conjugates where the main difference lies in the nature of the spacer and the type of bonds between the two components. The use of a long but flexible spacer afforded strong electronic GO-BODIPY interactions in the ground state. This drastically altered the light absorption of the BODIPY structure and impeded its selective excitation. In contrast, the utilisation of a short, but rigid spacer based on boronic esters resulted in a perpendicular geometry of the phenyl boronic acid BODIPY (PBA-BODIPY) with respect to the GO plane, which enables only minor electronic GO-BODIPY interactions in the ground state. In this case, selective excitation of PBA-BODIPY was easily achieved, allowing to investigate the excited state interactions. A quantitative ultrafast energy transfer from PBA-BODIPY to GO was observed. Furthermore, due to the reversible dynamic nature of the covalent GO-PBA-BODIPY linkage, some PBA-BODIPY is free in solution and, hence, not quenched from GO. This resulted in a weak, but detectable fluorescence from the PBA-BODIPY that will allow to exploit GO-PBA-BODIPY for slow release and imaging purposes.

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“…Among carbon nanomaterials, graphene oxide (GO) shows extensive multifactorial properties: stretchability, electrical and high thermal conductivity, and a large surface area that allows functionalisation with antimicrobial compounds. Moreover, GO has antimicrobial properties that can vary depending on the GO sheets’ dimension and the environment [ 20 , 21 ]. Briefly, GO displays three different antimicrobial mechanisms: GO sharp edges can physically cut the membrane, with the consequent leakage of the intracellular contents, GO can also induce the production of ROS, and, finally, GO sheets can wrap around microorganisms, isolating them from the environment, limiting the adsorption of nutrients, and stopping the proliferation [ 22 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Curcumin-Functionalized Graphene Oxide Strongly Prevents Candida parapsilosis Adhesion and Biofilm Formation

et al. 2023

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Candida parapsilosis is the major non-C. albicans species involved in the colonization of central venous catheters, causing bloodstream infections. Biofilm formation on medical devices is considered one of the main causes of healthcare-associated infections and represents a global public health problem. In this context, the development of new nanomaterials that exhibit anti-adhesive and anti-biofilm properties for the coating of medical devices is crucial. In this work, we aimed to characterize the antimicrobial activity of two different coated-surfaces, graphene oxide (GO) and curcumin-graphene oxide (GO/CU) for the first time, against C. parapsilosis. We report the capacity of GO to bind and stabilize CU molecules, realizing a homogenous coated surface. We tested the anti-planktonic activity of GO and GO/CU by growth curve analysis and quantification of Reactive Oxigen Species( ROS) production. Then, we tested the antibiofilm activity by adhesion assay, crystal violet assay, and live and dead assay; moreover, the inhibition of the formation of a mature biofilm was investigated by a viability test and the use of specific dyes for the visualization of the cells and the extra-polymeric substances. Our data report that GO/CU has anti-planktonic, anti-adhesive, and anti-biofilm properties, showing a 72% cell viability reduction and a decrease of 85% in the secretion of extra-cellular substances (EPS) after 72 h of incubation. In conclusion, we show that the GO/CU conjugate is a promising material for the development of medical devices that are refractory to microbial colonization, thus leading to a decrease in the impact of biofilm-related infections.

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Ball milled glyco-graphene oxide conjugates markedly disrupted Pseudomonas aeruginosa biofilms

Abstract: The engineering of the surface of nanomaterials with bioactive molecules allows controlling their biological identity thus accessing to functional materials with tuned physicochemical and biological profiles suited for specific applications....

Cited by 5 publications

References 68 publications

A Review of Top‐Down Strategies for the Production of Quantum‐Sized Materials

A Review of Top‐Down Strategies for the Production of Quantum‐Sized Materials

Graphene Oxide‐BODIPY Conjugates as Highly Fluorescent Materials

Curcumin-Functionalized Graphene Oxide Strongly Prevents Candida parapsilosis Adhesion and Biofilm Formation

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