Balbiani body, nuage and sponge bodies – The germ plasm pathway players

Kloc, Małgorzata; Jędrzejowska, Izabela; Tworzydło, Wacław; Biliński, S

doi:10.1016/j.asd.2013.12.003

Cited by 73 publications

(80 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In all animals examined, an evolutionarily conserved structure known as the Balbiani body (Bb) or mitochondrial cloud, forms in early oocytes (Kloc et al, 2014). The Bb is a non-membrane bound structure that contains organelles, proteins that in some vertebrates serves as a hub for GP assembly and to transport its cargo, including GP to the vegetal pole of oocytes (Kloc et al, 2014); however, the mechanisms underlying Bb function are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

“…The Bb is a non-membrane bound structure that contains organelles, proteins that in some vertebrates serves as a hub for GP assembly and to transport its cargo, including GP to the vegetal pole of oocytes (Kloc et al, 2014); however, the mechanisms underlying Bb function are poorly understood. The only gene known to be necessary and sufficient for Bb assembly in zebrafish or any other vertebrate thus far is bucky ball (buc) (Bontems et al, 2009; Heim et al, 2014; Marlow and Mullins, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

RETRACTED: Maternal dazap2 Regulates Germ Granules by Counteracting Dynein in Zebrafish Primordial Germ Cells

et al. 2015

View full text Add to dashboard Cite

Summary Primordial germ cells (PGCs) are the stem cells of the germline. Generally, germline induction occurs via zygotic factors or the inheritance of maternal determinants called germ plasm (GP). GP is packaged into ribonucleoprotein complexes within oocytes and later promotes the germline fate in embryos. Once PGCs are specified by either mechanism, GP components localize to perinuclear granular-like structures. Although components of zebrafish PGC germ granules have been studied, the maternal factors regulating their assembly and contribution to germ cell development are unknown. Here we show that the scaffold protein Dazap2 binds to Bucky ball, an essential regulator of oocyte polarity and GP assembly, and colocalizes with the GP in oocytes and in PGCs. Mutational analysis revealed a requirement for maternal Dazap2 (Mdazap2) in germ granule maintenance. Through molecular epistasis analyses we show that MDazap2 is epistatic to Tdrd7 and maintains germ granules in the embryonic germline by counteracting Dynein activity.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RETRACTED: Maternal dazap2 Regulates Germ Granules by Counteracting Dynein in Zebrafish Primordial Germ Cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In addition, we have established the role of SAD-2 as the likely scaffold protein for this complex. In higher eukaryotes, the perinuclear region in germ cells contains an organelle known as chromatoid body in mammals, nuage in flies, and P-granules in worms (Meikar et al 2011;Voronina 2013;Kloc et al 2014). By providing an environment in which RNAs can meet up with their developmental regulators, these perinuclear organelles play an important role in silencing, translation, and/or RNA processing/regulation/organization.…”

Section: Discussionmentioning

confidence: 99%

“…By providing an environment in which RNAs can meet up with their developmental regulators, these perinuclear organelles play an important role in silencing, translation, and/or RNA processing/regulation/organization. Certain silencing proteins (e.g., Argonaute) have been shown to congregate in these perinuclear regions, and their presence is required for specific RNA regulation and meiotic progression (Updike and Strome 2010;Meikar et al 2011;Kloc et al 2014). Conglomeration of related proteins may allow the coupling of consecutive reactions, thereby increasing the efficiency of the biochemical process.…”

Section: Discussionmentioning

confidence: 99%

Complex Formation of RNA Silencing Proteins in the Perinuclear Region of Neurospora crassa

et al. 2015

View full text Add to dashboard Cite

In Neurospora, genes not paired during meiosis are targeted by meiotic silencing by unpaired DNA (MSUD). Here, our bimolecular fluorescence complementation (BiFC) study suggests that RNA-directed RNA polymerase, Dicer, Argonaute, and others form a silencing complex in the perinuclear region, with intimate interactions among the majority of them. We have also shown that SAD-2 is likely the anchor for this assembly.

show abstract

“…The entire assembly forms a cytoplasmic structure in the oocyte named Balbiani body [18]. Sometimes it is also referred to as the mitochondrial cloud in Xenopus [19].…”

Section: Compositionmentioning

confidence: 99%

Germ Cell Specification: The Evolution of a Recipe to Make Germ Cells

Krishnakumar¹,

Dosch²

2018

Germ Cell

View full text Add to dashboard Cite

Multicellular species use gametes for their propagation. Gametes are formed from primordial germ cells (PGCs), which develop during embryogenesis. In some species, PGCs are speciied by the inheritance of a RNA granule known as germ plasm. During germ cell speciication, the germ plasm conveys a unique set of properties, e.g. the germ cell speciic meiotic cell cycle to the PGCs. Germ plasm assembly is controlled by independently evolving organizer proteins like Oskar in Drosophila or Bucky ball in zebraish. These organizers are intrinsically disordered proteins, which rapidly changed their amino acid sequence during evolution. A common recipe has emerged by studies on organizer proteins for animals that use germ plasm to specify their germline. Investigating the nature of these organizers might therefore provide a clue to germ cell speciication in other species, which are less accessible to molecular-genetic and embryological approaches. Moreover, we might understand how the irst metazoans modiied their existing cellular structures from unicellular eukaryotes to ensure their reproduction.

show abstract

Balbiani body, nuage and sponge bodies – The germ plasm pathway players

Cited by 73 publications

References 52 publications

RETRACTED: Maternal dazap2 Regulates Germ Granules by Counteracting Dynein in Zebrafish Primordial Germ Cells

RETRACTED: Maternal dazap2 Regulates Germ Granules by Counteracting Dynein in Zebrafish Primordial Germ Cells

Complex Formation of RNA Silencing Proteins in the Perinuclear Region of Neurospora crassa

Germ Cell Specification: The Evolution of a Recipe to Make Germ Cells

Contact Info

Product

Resources

About