Balancing selection and wild gene pool contribute to resistance in global rice germplasm against planthopper

Zhou, Cong; Zhang, Qian; Chen, Yu; Huang, Jin; Guo, Qin; Li, Yang; Wang, Wensheng; Qiu, Yongfu; Guan, Wanqing; Zhang, Jing; Guo, Jianping; Shi, Shaojie; Wu, Di; Zheng, Xiaohui; Nie, Lingyun; Tan, Jiaoyan; Huang, Chao; Ma, Yinhua; Yang, Fang; Fu, Xiqin; Du, Bo; Zhu, Lili; Chen, Rongzhi; Li, Zhikang; Yuan, Longping; He, Guangming

doi:10.1111/jipb.13157

Cited by 26 publications

(32 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alternatively, the breeding and cultivation of resistant rice cultivars is more effective and environmentally friendly. In the past few decades, over 40 BPH resistance genes have been identified in rice, 11 of which have been successfully cloned [ 6 , 7 , 8 , 9 ]. Nonetheless, how these genes confer effective BPH resistance remain elusive, as reports on the mechanism underlying BPH resistance have been scarce.…”

Section: Introductionmentioning

confidence: 99%

Cytokinin Confers Brown Planthopper Resistance by Elevating Jasmonic Acid Pathway in Rice

Zhang

Liu

Gao

et al. 2022

IJMS

View full text Add to dashboard Cite

Plants have evolved a sophisticated defense system that employs various hormone pathways to defend against attacks by insect pests. Cytokinin (CK) plays an important role in plant growth and stress tolerance, but the role of CKs in plant–insect interaction remains largely unclear. Here, we report that CKs act as a positive regulator in rice resistance against brown planthopper (BPH), a devastating insect pest of rice. We found that BPH feeding promotes CK biosynthesis and signaling in rice. Exogenous application of CKs significantly increased the rice resistance to BPH. Increasing endogenous CKs by knocking out cytokinin oxidase/dehydrogenase (OsCKXs) led to enhanced resistance to BPH. Moreover, the levels of the plant hormone jasmonic acid (JA) and the expression of JA-responsive genes were elevated by CK treatment and in OsCKXs knockout plants. Furthermore, JA-deficient mutant og1 was more susceptible to BPH, and CK-induced BPH resistance was suppressed in og1. These results indicate that CK-mediated BPH resistance is JA-dependent. Our findings provide the direct evidence for the novel role of CK in promoting insect resistance, and demonstrate that CK-induced insect resistance is JA-dependent. These results provide important guidance for effective pest management strategies in the future.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytokinin Confers Brown Planthopper Resistance by Elevating Jasmonic Acid Pathway in Rice

Zhang

Liu

Gao

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Rice varieties that are resistant to BPH can inhibit the pest’s feeding, growth, and fecundity ( Zheng et al, 2021 ). In recent decades, many BPH resistance genes and QTLs have been detected in cultivated rice and wild species ( Guo et al, 2018 ; Shi et al, 2021 ; Zhou et al, 2021 ). However, new BPH biotypes may evolve that are virulent to resistant rice varieties ( Claridge and Den Hollander, 1980 ; Saxena et al, 1991 ).…”

Section: Introductionmentioning

confidence: 99%

Bulked Segregant RNA Sequencing Revealed Difference Between Virulent and Avirulent Brown Planthoppers

et al. 2022

Self Cite

View full text Add to dashboard Cite

The brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most devastating insect pests of rice (Oryza sativa L.), but BPH populations have varying degrees of virulence to rice varieties carrying different resistance genes. To help efforts to characterize these variations we applied bulked segregant RNA sequencing (BSR-seq) to identify differentially expressed genes (DEGs) and genetic loci associated with BPH virulence to YHY15 rice plants carrying the resistance gene Bph15. BPHs that are highly virulent or avirulent to these plants were selected from an F2 population to form two contrasting bulks, and BSR-seq identified 751 DEGs between the bulks. Genes associated with carbohydrate, amino acid and nucleotide metabolism, the endocrine system, and signal transduction were upregulated in the avirulent insects when they fed on these plants. The results also indicated that shifts in lipid metabolism and digestive system pathways were crucial for the virulent BPHs’ adaptation to the resistant rice. We identified 24 single-nucleotide polymorphisms (SNPs) in 21 genes linked with BPH virulence. Possible roles of genes apparently linked to BPH virulence are discussed. Our results provide potentially valuable information for further studies of BPH virulence mechanisms and development of robust control strategies.

show abstract

“…Previous genome‐wide association analyses identified 3502 SNPs and 59 loci associated with BPH resistance in rice (Zhou et al ., 2021), which may have important implications for the control of BPH. Here, we observed that SNP8 and SNP9 in the OsCslF6 promoter produced an AT‐rich domain in the AA allele, but not in the AT or TT alleles (Figure 4a,b).…”

Section: Discussionmentioning

confidence: 99%

Poaceae‐specific β‐1,3;1,4‐d‐glucans link jasmonate signalling to OsLecRK1‐mediated defence response during rice‐brown planthopper interactions

Dai

Liu

Guo

et al. 2023

Plant Biotechnology Journal

View full text Add to dashboard Cite

Brown planthopper (BPH, Nilaparvata lugens), a highly destructive insect pest, poses a serious threat to rice (Oryza sativa) production worldwide. Jasmonates are key phytohormones that regulate plant defences against BPH; however, the molecular link between jasmonates and BPH responses in rice remains largely unknown. Here, we discovered a Poaceae-specific metabolite, mixed-linkage b-1,3;1,4-D-glucan (MLG), which contributes to jasmonatemediated BPH resistance. MLG levels in rice significantly increased upon BPH attack. Overexpressing OsCslF6, which encodes a glucan synthase that catalyses MLG biosynthesis, significantly enhanced BPH resistance and cell wall thickness in vascular bundles, whereas knockout of OsCslF6 reduced BPH resistance and vascular wall thickness. OsMYC2, a master transcription factor of jasmonate signalling, directly controlled the upregulation of OsCslF6 in response to BPH feeding. The AT-rich domain of the OsCslF6 promoter varies in rice varieties from different locations and natural variants in this domain were associated with BPH resistance. MLG-derived oligosaccharides bound to the plasma membrane-anchored LECTIN RECEPTOR KINASE1 OsLecRK1 and modulated its activity. Thus, our findings suggest that the OsMYC2-OsCslF6 module regulates pest resistance by modulating MLG production to enhance vascular wall thickness and OsLecRK1-mediated defence signalling during rice-BPH interactions.

show abstract

Balancing selection and wild gene pool contribute to resistance in global rice germplasm against planthopper

Cited by 26 publications

References 72 publications

Cytokinin Confers Brown Planthopper Resistance by Elevating Jasmonic Acid Pathway in Rice

Cytokinin Confers Brown Planthopper Resistance by Elevating Jasmonic Acid Pathway in Rice

Bulked Segregant RNA Sequencing Revealed Difference Between Virulent and Avirulent Brown Planthoppers

Poaceae‐specific β‐1,3;1,4‐d‐glucans link jasmonate signalling to OsLecRK1‐mediated defence response during rice‐brown planthopper interactions

Contact Info

Product

Resources

About