Balanced Laser Transmission Spectroscopy Based on a Tunable Gain Double Channel LIA for Nanoparticles Detection in Biomedical Applications

Marcellis, Andrea De; Sarra, Angelo; Stanchieri, Guido Di Patrizio; Bruni, Fabio; Bordi, F.; Palange, E.; Postorino, P.

doi:10.1109/biocas.2019.8918741

Cited by 3 publications

(5 citation statements)

References 14 publications

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“…Finally, this paper extends the preliminary work reported in [40] providing additional theoretical and technical details, improved circuit design and system implementation, a deeper data analysis and further experimental measurements using both standard and biological nanoparticles.…”

Section: Introductionsupporting

confidence: 61%

Laser Transmission Spectroscopy Based on Tunable-Gain Dual-Channel Dual-Phase LIA for Biological Nanoparticles Characterization

Sarra

Stanchieri

Marcellis

et al. 2021

IEEE Trans. Biomed. Circuits Syst.

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Size and absolute concentration of suspensions of nanoparticles are important information for the study and development of new materials and products in different industrial applications spanning from biotechnology and pharmaceutics to food preparation and conservation. Laser Transmission Spectroscopy (LTS) is the only methodology able to measure nanoparticle size and concentration by performing a single measurement. In this paper we report on a new variable gain calibration procedure for LTS-based instruments allowing to decrease of an order of magnitude the experimental indetermination of the particle size respect to the conventional LTS based on the double ratio technique. The variable gain calibration procedure makes use of a specifically designed tunable-gain, dual-channel, dual-phase Lock-In Amplifier (LIA) whose input voltage signals are those ones generated by two Si photodiodes that measure the laser beam intensities passing through the sample containing the nanoparticles and a reference optical path. The LTS variable gain calibration procedure has been validated by firstly using a suspension of NIST standard polystyrene nanoparticles even 36 hours after the calibration procedure was accomplished. The paper reports in detail the LIA implementation describing the design methodologies and the electronic circuits. As a case example of the characterization of biological nanostructures, we demonstrate that a single LTS measurement allowed to determine size density distribution of a population of extracellular vesicles extracted from orange juice (25 nm in size) with the presence of their aggregates having a size of 340 nm and a concentration smaller than 3 orders of magnitude.

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Section: Introductionsupporting

confidence: 61%

Laser Transmission Spectroscopy Based on Tunable-Gain Dual-Channel Dual-Phase LIA for Biological Nanoparticles Characterization

Sarra

Stanchieri

Marcellis

et al. 2021

IEEE Trans. Biomed. Circuits Syst.

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“…A big advantage of LTS over the more traditional diffusion techniques, such as DLS and Nanoparticle Tracking Analysis (NTA), is that this method furnishes the distribution of the geometrical sizes (and in principle the shape) of the suspended particles and not their equivalent "hydrodynamic" radii (radius of a sphere with the same diffusion coefficient), and moreover their absolute concentration in the suspension (not the relative weight of the different size classes). The LTS technique measures the transmittance of a laser beam through the aqueous suspension of vesicles as a function of the wavelength [28,29]. Given the transmission coefficient T(λ) and the known wavelength-size dependent properties, i.e., Mie scattering cross-section σ(λ,r) of the vesicles, represented in the present case as shelled spheres [30], the particle density distribution n(r) as a function of their size can be calculated through the Beer-Lambert law [29].…”

Section: Isolation and Characterization Of B-elnsmentioning

confidence: 99%

“…The LTS technique measures the transmittance of a laser beam through the aqueous suspension of vesicles as a function of the wavelength [28,29]. Given the transmission coefficient T(λ) and the known wavelength-size dependent properties, i.e., Mie scattering cross-section σ(λ,r) of the vesicles, represented in the present case as shelled spheres [30], the particle density distribution n(r) as a function of their size can be calculated through the Beer-Lambert law [29]. Transmission data are analyzed and inverted by using a mean square root-based algorithm giving the particle size distribution in terms of their absolute concentration.…”

Section: Isolation and Characterization Of B-elnsmentioning

confidence: 99%

Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

et al. 2020

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Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features. In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α). Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes; and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo. In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactive compounds potentially able to protect vascular system against various stressors.

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“…The size and the absolute number concentration of the liposomal suspension were determined using an innovative and customized apparatus implementing the laser transmission spectroscopy (LTS) technique [33,34]. Since it is relatively new and probably unfamiliar to the readership, we will give here a very brief account of this technique.…”

Section: Laser Transmission Spectroscopymentioning

confidence: 99%

“…Transmission data are analyzed and inverted by using a mean square root-based algorithm, giving the particle size distribution in terms of their absolute concentration. The integral of the density distribution provides the total number of liposomes per milliliter of solution N LT S [34,35]. The volume fraction Φ in of the liposomal dispersion available for encapsulation can be hence calculated as 3 , where d is the bilayer thickness.…”

Section: Laser Transmission Spectroscopymentioning

confidence: 99%

Influence of drug/lipid interaction on the entrapment efficiency of isoniazid in liposomes for antitubercular therapy: a multi-faced investigation

Sciolla¹,

Truzzolillo²,

Chauveau³

et al. 2021

Preprint

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Hypothesis.Isoniazid is one of the primary drugs used in tuberculosis treatment. Isoniazid encapsulation in liposomal vesicles can improve drug therapeutic index and minimize toxic and side effects. In this work, we consider mixtures of hydrogenated soy phosphatidylcholine/phosphatidylglycerol (HSPC/DPPG) to get novel biocompatible liposomes for isoniazid pulmonary delivery. Our goal is to understand if the entrapped drug affects bilayer structure.Experiments. HSPC-DPPG unilamellar liposomes are prepared and characterized by dynamic light scattering, ζ-potential, fluorescence anisotropy and Transmission Electron Microscopy. Isoniazid encapsulation is determined by UV and Laser Transmission Spectroscopy. Calorimetry, light scattering and Surface Pressure measurements are used to get insight on adsorption and thermodynamic properties of lipid bilayers in the presence of the drug. Findings.We find that INH-lipid interaction can increase the entrapment capability of the carrier due to isoniazid adsorption. The preferential INH-HSPC dipole-dipole interaction promotes modification of lipid packing and ordering and favors the condensation of a HSPC-richer phase in molar excess of DPPG. Our findings highlight the importance of fundamental investigations of drug-lipid interactions for the optimal design of liposomal nanocarriers.

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Balanced Laser Transmission Spectroscopy Based on a Tunable Gain Double Channel LIA for Nanoparticles Detection in Biomedical Applications

Cited by 3 publications

References 14 publications

Laser Transmission Spectroscopy Based on Tunable-Gain Dual-Channel Dual-Phase LIA for Biological Nanoparticles Characterization

Laser Transmission Spectroscopy Based on Tunable-Gain Dual-Channel Dual-Phase LIA for Biological Nanoparticles Characterization

Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells

Influence of drug/lipid interaction on the entrapment efficiency of isoniazid in liposomes for antitubercular therapy: a multi-faced investigation

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