Bakterien und Virus in Übermikroskopischer Aufnahme

Borries, Bodo von; Руска, Э.; Rüska, H.

doi:10.1007/bf01775798

Cited by 78 publications

(28 citation statements)

References 9 publications

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“…filtration and sedimentation. By applying EM, virology made a great step forward, as it allowed the direct demonstration of the particulate nature of viruses as well as the description of their size and morphology, shown already by the first electron micrographs of poxviruses (von Borries et al, 1938). Within a short period of time a number of additional viruses and bacteriophages, as well as bacteria, were morphologically characterized (Kausche et al, 1939;Ruska et al, 1939;Ruska, 1940a,b;Luria et al, 1943) making insights into structure-function relations and classification of viruses possible.…”

Section: Past and Present Of Diagnostic Electron Microscopymentioning

confidence: 99%

Diagnostic electron microscopy is still a timely and rewarding method

Biel

Gelderblom

1999

Journal of Clinical Virology

116

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In the history of diagnostic EM in virology, a change in use has been seen. Starting in the 1990s and coincident with the broad introduction of 'modern' diagnostic techniques, the number of EM diagnostic labs has decreased considerably--in spite of the obvious advantages of this technique. To guarantee the continuing performance of diagnostic EM in the future. EQA runs have to be performed as with other techniques in the diagnostic armament. The growing number of participants and participating countries indicates an interest in as well as a need for this program.

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Section: Past and Present Of Diagnostic Electron Microscopymentioning

confidence: 99%

Diagnostic electron microscopy is still a timely and rewarding method

Biel

Gelderblom

1999

Journal of Clinical Virology

116

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“…Electron microscopy (EM) can be used to visualize virus particles directly at high magnifications. The first investigations of the morphology and later the ultrastructure of viruses infecting humans and animals became possible, due to the invention of the EM technique in the early 1930s (Agar, 1996), and the first micrographs showing the size and morphology of poxviruses were published in 1938 (Von Borries et al, 1938). With the development of the negative contrast staining method by Brenner and Horne (1959), viral particles could be investigated at the ultrastructural level.…”

Section: Introductionmentioning

confidence: 99%

Confirmation of electron microscopy results by direct testing of viruses adhered to grids using nucleic acid amplification techniques

Johnsen

Böttiger

Blom

2006

Journal of Virological Methods

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It is possible to visualize rapidly viral particles by electron microscopy (EM) in patient samples and in cell cultures, and characterize the particles on the basis of their size and morphology. In many instances, EM has contributed to the diagnosis of specific infectious agents. Four different types of viruses with different characteristics of particle size, capsid structure, the presence or absence of an envelope, genomic content and stability outside the host were screened and diagnosed by EM at the level of family/genus. The results were confirmed at the species level by elution of the sample material from the grids used for EM examination and nucleic acid amplification. This approach could be valuable in situations where the immediate diagnosis is unclear, or when new infectious agents appear.

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“…The very thick capsules of relatively low electron density, seen to surround brucellas by earlier electron microscopists (e.g. von Borries, Ruska & Ruska, 1938;Lembke, Kornlein & Frahm, 1950;Malfatti & Ithurralde, 1951) may well have been due to experimental artifacts. Their observations were made, in fact, on whole exsiccated bacteria lying on supporting films.…”

Section: Discussionmentioning

confidence: 91%

The Ultrastructure of S and R Variants of Brucella abortus Grown on a Lifeless Medium

Petris

Karlsbad

Kessel

1964

Journal of General Microbiology

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SUMMARYAn electron microscopic investigation was carried out on thin sections of both S and R variants of Brucella abortus, strain S A : no ultrastructural differences were noted between the two variants.The cells are limited by a cell wall, comprising an outer triple-layered membrane (65-80 8. thick) and an inner homogeneous layer of variable thickness, and a triple-layered cytoplasmic membrane about 70 A. thick.The cytoplasm and nucleoplasm of brucellae are similar to those of other bacteria. A characteristic feature of these organisms is the presence of membranous structures (300-800 A. in diameter) associated with the cytoplasmic membrane. These 'peripheral formations ', which occur singly or in clusters, do not appear to arise by obvious invagination of the cytoplasmic membrane. No evidence has been obtained for the existence of capsules in virulent brucellae. INTROIIUC'l'IOISThe aim of the present investigation was to study the ultrastructure of Brucella abortus grown either on a lifeless medium or within monocytes maintained in vitro. To our knowledge no detailed study of this organism, employing present-day techniques of electron microscopy, has been published to date.The first paper in this series deals with the morphology of S and R variants of BruceEEa abortus strain S A , grown on a lifeless medium. Our interest in comparing both variants stemmed from the notion that the mutational event occurring in B. abortus, which results in a change of genotype from S to non S (e.g. R or M), is associated with a variety of phenotypic changes. These include loss of or decrease in virulence, loss of the ability to multiply intracellularly (Braun, Pomales-Ldbron & Stinebring, 1958), change in colonial morphology from smooth to non-smooth (e.g. rough or mucoid), change in surface properties resulting in agglutination, change from sensitivity to resistance to brucella phage (Stinebring & Braun, 1959), and changes in antigenic composition. These alterations, and particularly the last four, suggested that morphological changes may occur in the surface structure of the bacteria. It seemed interesting therefore to investigate whether the above phenotypic changes would be reflected in ultrastructural differences detectable with the electron microscope.

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Bakterien und Virus in Übermikroskopischer Aufnahme

Cited by 78 publications

References 9 publications

Diagnostic electron microscopy is still a timely and rewarding method

Diagnostic electron microscopy is still a timely and rewarding method

Confirmation of electron microscopy results by direct testing of viruses adhered to grids using nucleic acid amplification techniques

The Ultrastructure of S and R Variants of Brucella abortus Grown on a Lifeless Medium

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