Baicalin modulates apoptosis via RAGE, MAPK, and AP-1 in vascular endothelial cells during <i>Haemophilus parasuis</i> invasion

Fu, Shulin; Zhao, Wenhua; Xiong, Chunhong; Li-wei, Guo; Guo, Jing; Qiu, Yinsheng; Hu, Chien‐An Andy; Chun, Youngsub; Liu, Yu; Wu, Zhongyuan; Hou, Yongqing

doi:10.1177/1753425919856078

Cited by 12 publications

(13 citation statements)

References 42 publications

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“…Moreover, TIMP1 also plays an important role of anti-in ammatory and antinociceptive 18 , indicating that TIMP1 may be a critical component of a signalling cascade involved in reducing in ammatory hypersensitivity. H.parasuis infection activated the in ammatory signaling molecules and produced several pro-in ammatory cytokines in porcine cells 19,20 . Additionally, highly virulent H. parasuis infection increased a proportion of CD163 + monocytes in pigs, which are able to produce high amounts of proin ammatory cytokines, such as TNF-α, IL-1 and IL-6 21 .…”

Section: Discussionmentioning

confidence: 99%

Integrative gene network analysis identifies TIMP1 as key factors for Haemophilus parasuis infection

Xia¹,

Liu²,

Chen³

et al. 2021

Preprint

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Haemophilus parasuis (H.parasuis), an important swine pathogen, causes Glässer's disease leading to pulmonary fibrosis, polyserositis, meningitis, and arthritis. However, the common molecular response and reaction from the host remain unknown. In this study, to uncover novel host factors involved in H.parasuis infection, we identified the global transcriptomics of porcine lung, spleen, blood, alveolar macrophages (PAM), peripheral blood mononuclear cell (PBMC) and aortic vascular endothelial cells (PAVECs) after infection of H.parasuis (Hps0165 strains) using microarray data and high throughput sequencing from Gene Expression Omnibus (GEO), respectively. The results showed that fifteen overlapped genes were significantly regulated in H.parasuis infected porcine lung and spleen, and then were compared with the data from porcine blood, revealing RETN, TIMP1 and C4BPA play potentially an important role for H.parasuis invasion. Furthermore, through analysing porcine cells infected with H.parasuis, we uncover the only overlap gene TIMP1 remarkably upregulated in all assembled data, indicating that TIMP1 could function as key target for the treatment of H.parasuis infection.

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Section: Discussionmentioning

confidence: 99%

Integrative gene network analysis identifies TIMP1 as key factors for Haemophilus parasuis infection

Xia¹,

Liu²,

Chen³

et al. 2021

Preprint

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“…HMGB1 also participates in systemic fibrotic diseases through the RAGE/MAPK and NF-κB signalling pathways [ 42 ]. In a previous study, we showed that G. parasuis stimulated the release of HMGB1 in piglet PBMNs [ 22 ] and induced the activation of the RAGE and MAPK signalling pathways in porcine aortic vascular endothelial cells [ 20 ]. Therefore, we investigated whether HMGB1 is released and the MAPK signalling pathway is activated in piglets after challenge with G. parasuis .…”

Section: Discussionmentioning

confidence: 99%

“…Cell apoptosis is a type of programmed cell death that may contribute to the orderly and efficient removal of damaged cells [ 44 ]. In a previous study, we also showed that baicalin inhibits the apoptosis induced by G. parasuis via the RAGE and MAPK signalling pathways in vascular endothelial cells [ 20 ]. However, whether baicalin attenuates apoptosis in piglets challenged with G. parasuis has not been investigated until now.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous work, we found that baicalin could suppress the NF-κB and NLRP3 inflammasome signalling pathways induced by G. parasuis in porcine aortic vascular endothelial cells (PAVECs) [ 18 ] and piglet mononuclear phagocytes (PMNPs) [ 19 ]. Baicalin reduced apoptosis triggered by G. parasuis via RAGE, MAPK, and AP-1 in PAVECs [ 20 ]. Baicalin also inhibited PKC-MAPK signalling pathway activation [ 21 ] and attenuated high-mobility group box 1 (HMGB1) secretion [ 22 ] in PMNPs stimulated by G. parasuis .…”

Section: Introductionmentioning

confidence: 99%

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The effects of baicalin on piglets challenged with Glaesserella parasuis

Yin

Zuo

et al. 2020

Vet Res

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Glaesserella parasuis ( G. parasuis ) causes porcine vascular inflammation and damage. Baicalin is reported to have antioxidant and anti-inflammatory functions. However, whether baicalin protects piglets against G. parasuis challenge and the potential protective mechanism have not been investigated. Therefore, in this study, we comprehensively examined the protective efficacy of baicalin in piglets challenged with G. parasuis and the possible protective mechanism. Our results show that baicalin attenuated the release of the inflammation-related cytokines interleukin (IL) 1β, IL6, IL8, IL10, and tumour necrosis factor α (TNF-α) and reduced high mobility group box 1 (HMGB1) production and cell apoptosis in piglets infected with G. parasuis . Baicalin also inhibited the activation of the mitogen-activated protein kinase (MAPK) signalling pathway and protected piglets against G. parasuis challenge. Taken together, our data suggest that baicalin could protect piglets from G. parasuis by reducing HMGB1 release, attenuating cell apoptosis, and inhibiting MAPK signalling activation, thereby alleviating the inflammatory response induced by the bacteria. Our results suggest that baicalin has utility as a novel therapeutic drug to control G. parasuis infection.

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“…Baicalin could inhibit the production of IL-6, IL-8, IL-10 and TNF-α and the phosphorylation of ERK, JNK and p38. Thereby activating the immune response induced by Th-1 to promoting the elimination of bacteria [ 105 ]. To sum up, SB play roles of anti-inflammatory, antibacterial and anti-viral via immune response.…”

Section: Pharmacological Effects (Shown In Table 2 mentioning

confidence: 99%

Applications, phytochemistry, pharmacological effects, pharmacokinetics, toxicity of Scutellaria baicalensis Georgi. and its probably potential therapeutic effects on COVID-19: a review

et al. 2020

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Scutellaria baicalensis Georgi. (SB) is a common heat-clearing medicine in traditional Chinese medicine (TCM). It has been used for thousands of years in China and its neighboring countries. Clinically, it is mostly used to treat diseases such as cold and cough. SB has different harvesting periods and processed products for different clinical symptoms. Botanical researches proved that SB included in the Chinese Pharmacopoeia (1st, 2020) was consistent with the medicinal SB described in ancient books. Modern phytochemical analysis had found that SB contains hundreds of active ingredients, of which flavonoids are its major components. These chemical components are the material basis for SB to exert pharmacological effects. Pharmacological studies had shown that SB has a wide range of pharmacological activities such as antiinflammatory, antibacterial, antiviral, anticancer, liver protection, etc. The active ingredients of SB were mostly distributed in liver and kidney, and couldn't be absorbed into brain via oral absorption. SB’s toxicity was mostly manifested in liver fibrosis and allergic reactions, mainly caused by baicalin. The non-medicinal application prospects of SB were broad, such as antibacterial plastics, UV-resistant silk, animal feed, etc. In response to the Coronavirus Disease In 2019 (COVID-19), based on the network pharmacology research, SB’s active ingredients may have potential therapeutic effects, such as baicalin and baicalein. Therefore, the exact therapeutic effects are still need to be determined in clinical trials. SB has been reviewed in the past 2 years, but the content of these articles were not comprehensive and accurate. In view of the above, we made a comprehensive overview of the research progress of SB, and expect to provide ideas for the follow-up study of SB.

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Baicalin modulates apoptosis via RAGE, MAPK, and AP-1 in vascular endothelial cells during Haemophilus parasuis invasion

Cited by 12 publications

References 42 publications

Integrative gene network analysis identifies TIMP1 as key factors for Haemophilus parasuis infection

Integrative gene network analysis identifies TIMP1 as key factors for Haemophilus parasuis infection

The effects of baicalin on piglets challenged with Glaesserella parasuis

Applications, phytochemistry, pharmacological effects, pharmacokinetics, toxicity of Scutellaria baicalensis Georgi. and its probably potential therapeutic effects on COVID-19: a review

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