Baicalin Attenuates Acute Myocardial Infarction of Rats <i>via</i> Mediating the Mitogen-Activated Protein Kinase Pathway

Abstract

Baicalin is a bioactive ingredient from the herb and has possessed various pharmacological actions. The present study was performed to evaluate the cardioprotective potential of baicalin against myocardial infarction and explore the potential mechanism. Baicalin was intraperitoneally injected into the rats by the doses of 50, 100 and 200 mg/kg, respectively, once a day for 7 d and, 30 min after the last administration, the left coronary artery was ligated. Infarct size was measured to analyze the myocardial damage. Myocardial specific enzymes, including creatine kinase (CK), the MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) were determined with the colorimetric method. Evidence for myocardial apoptosis was detected by caspase-3 activity measurement and Western blot analysis. We also examined the protein levels of three major subgroups of mitogen-activated protein kinases (MAPKs), namely, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 by immuoblotting. Our results indicated that baicalin significantly reduced the infarct size and myocardial enzymes (CK, CK-MB, LDH and cTnT). Administration of baicalin also suppressed the activity and protein expression of caspase-3. Moreover, the protein level of phosphorylated ERK (p-ERK) was found to be evidently augmented while the phosphorylated JNK (p-JNK) and phosphorylated p38 (p-p38) were strikingly diminished in infarcted rats with baicalin treatment. These findings suggest that the baicalin's cardioprotection associates with mediation of MAPK cascades in acute myocardial infarction of rats.

Baicalin Maintains Late-Stage Functional Cardiomyocytes in Embryoid Bodies Derived from Murine Embryonic Stem Cells

“…Several groups reported that baicalin induced apoptosis of cancer cells [46] and carcinoma cells [47], via ERK and p38 MAPK signaling pathways. However, baicalin also showed cytoprotective effects on CMs [48,49], hepatocytes and neural cells [50,51,52]. A latest study reported that baicalin attenuates acute myocardial infarction of rats via mediating ERK and p38 MAPK signaling pathways [48].…”

“…However, baicalin also showed cytoprotective effects on CMs [48,49], hepatocytes and neural cells [50,51,52]. A latest study reported that baicalin attenuates acute myocardial infarction of rats via mediating ERK and p38 MAPK signaling pathways [48]. In this latest study, baicalin treatment significantly augments the phosphorylated ERK (p-ERK) while dramatically diminishes the phosphorylated JNK (p-JNK) and p38 (p-p38) in rats with myocardial infarction [48].…”

“…A latest study reported that baicalin attenuates acute myocardial infarction of rats via mediating ERK and p38 MAPK signaling pathways [48]. In this latest study, baicalin treatment significantly augments the phosphorylated ERK (p-ERK) while dramatically diminishes the phosphorylated JNK (p-JNK) and p38 (p-p38) in rats with myocardial infarction [48]. Therefore, we propose that baicalin selectively induces the death of specific cell types but not CMs in EBs during differentiation, giving rise to smaller EBs with relative higher proportion of ES-CMs.…”

Baicalein protects against the development of angiotensin II-induced abdominal aortic aneurysms by blocking JNK and p38 MAPK signaling

“…Baicalein (BAI) , which is involved in oxidative stress, inflammation, tumorigenesis, and angiogenesisis, is the main component of the Chinese traditional drug "Huang Qin", (Gao et al, 1999). It has been reported that BAI remarkably retards is chemia/reperfusion-induced myocardium injury (Liu et al, 2013;Wang et al, 2013). However, its exact mechanism remains elusive and its role in AAA development has not been elucidated.…”

Protective effects on myocardial infarction model: delivery of schisandrin B using matrix metalloproteinase-sensitive peptide-modified, PEGylated lipid nanoparticles

“…43 In this study, cardiac protective effects of Sch B loaded SLNs were evaluated using an acute MI model. MMP-Sch B SLNs …”