Baicalin alleviates adriamycin-induced focal segmental glomerulosclerosis and proteinuria by inhibiting the Notch1-Snail axis mediated podocyte EMT

Dou, Yitian; Yichun, Shang; Shen, Yongmei; Qu, Jingtian; Liu, Chunliu; Cao, Jiasong

doi:10.1016/j.lfs.2020.118010

Cited by 16 publications

(12 citation statements)

References 31 publications

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“…In the present study, BNIP3L upregulated the expressions of Bax and C-capase-3, downregulated that of Bcl-2 and partly reversed the effects of miR-23b-3p. Additionally, EMT is one of the responses of podocytes to injury resulting in the depletion of podocytes, and blocked EMT can alleviate the injury of podocytes [ 44 ]. The upregulation of N-cadherin and the downregulation of E-cadherin are hallmarks of EMT [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Nuclear paraspeckle assembly transcript 1 promotes the podocyte injury via targeting miR-23b-3p/B-cell lymphoma-2 interacting protein 3 like axis

Wang

Luo

et al. 2022

Renal Failure

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Background Given the reported effects of nuclear paraspeckle assembly transcript 1 (NEAT1) on kidney injury, a study is worth formulating to investigate whether and how NEAT1 impacts podocytes. Materials and methods A mouse podocyte injury model was established using the adriamycin (ADR)-induced mouse podocyte cell line (MPC5). The target relationships between NEAT1 and microRNA (miR)-23b-3p and between miR-23b-3p and Bcl-2 interacting protein 3 like (BNIP3L) were verified by dual-luciferase reporter assay and RNA immunoprecipitation assay. After ADR-induced MPC5 cells were transfected with NEAT1 overexpression plasmid (oe-NEAT1) or shNEAT1, the viability and apoptosis of MPC5 cells were evaluated by Cell Counting Kit-8 (CCK-8) assay and flow cytometry, respectively. The expressions of MPC5, miR-23b-3p, BNIP3L and the factors related to podocyte injury, apoptosis and epithelial-mesenchymal transition were determined using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Results NEAT1 was high-expressed in ADR-induced cell model. After transfection with oe-NEAT1, the expression of NEAT1, the levels of marker (Desmin) and apoptosis were promoted, while the viability and the levels of podocyte injury markers (WT1, Nephrin) were inhibited in ADR-induced cells. However, shNEAT1 generated the effects opposite to oe-NEAT1. Besides, miR-23b-3p competitively bound to NEAT1 and targeted BNIP3L. MiR-23b-3p inhibitor reversed the effect of shNEAT1, while its effect could be further offset by shBNIP3L. Furthermore, miR-23b-3p inhibitor affected mouse podocyte injury through downregulating Bcl-2 and E-cadherin levels and upregulating Cleaved-caspase-3, Bax, N-cadherin, Vimentin and Snail levels, but shBNIP3L did oppositely. Conclusion NEAT1 promotes the podocyte injury via targeting miR-23b-3p/BNIP3L axis.

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Section: Discussionmentioning

confidence: 99%

Nuclear paraspeckle assembly transcript 1 promotes the podocyte injury via targeting miR-23b-3p/B-cell lymphoma-2 interacting protein 3 like axis

Wang

Luo

et al. 2022

Renal Failure

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“…Baicalin suppressed Notch1-Snail pathway activation in podocytes and alleviated glomerulus structural disruption and dysfunction in adriamycin (ADR)-induced nephropathy [ 184 ]. Total flavonoids in Astragali Radix (AR) were reported to protect against ADR-induced nephropathy related to the protection of renal filtration function and regulation of blood pressure, which might involve the regulation of the immune system and RAAS [ 185 ].…”

Section: Flavonoids In Ckdmentioning

confidence: 99%

Flavonoids in Treatment of Chronic Kidney Disease

et al. 2022

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Chronic kidney disease (CKD) is a progressive systemic disease, which changes the function and structure of the kidneys irreversibly over months or years. The final common pathological manifestation of chronic kidney disease is renal fibrosis and is characterized by glomerulosclerosis, tubular atrophy, and interstitial fibrosis. In recent years, numerous studies have reported the therapeutic benefits of natural products against modern diseases. Substantial attention has been focused on the biological role of polyphenols, in particular flavonoids, presenting broadly in plants and diets, referring to thousands of plant compounds with a common basic structure. Evidence-based pharmacological data have shown that flavonoids play an important role in preventing and managing CKD and renal fibrosis. These compounds can prevent renal dysfunction and improve renal function by blocking or suppressing deleterious pathways such as oxidative stress and inflammation. In this review, we summarize the function and beneficial properties of common flavonoids for the treatment of CKD and the relative risk factors of CKD.

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“…Li et al reported that treatment with BA can significantly promote the viability of podocytes, decrease HG-induced podocyte apoptosis, and finally serve a protective role in a dose-dependent manner . Dou et al investigated the effects of BA on ADR-induced rats and TGF-β1-induced podocytes, and they found that BA reduces proteinuria, relieves glomerulus structural disruption and dysfunction by inhibiting the expressions of podocyte EMT markers and cell migration (Dou et al, 2020). Qu et al proposed that BA can slow down the damage of podocytes caused by hyperglycemia, and the underlying mechanism is closely related to the suppression of PI3K/ AKT/mTOR signaling pathway (Qu et al, 2021).…”

Section: Baicalinmentioning

confidence: 99%

“…diet and STZ-induced podocyte injury in rats; HGinduced podocyte in cells; ADRinduced podocyte injury in rats and TGF-β1-induced podocyte in cells,Dou et al (2020), Qu et al (2021 …”

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confidence: 99%

Recent Advances in Traditional Chinese Medicine for Treatment of Podocyte Injury

Yao

Han

et al. 2022

Front. Pharmacol.

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Podocyte is also called glomerular epithelial cell, which has been considered as the final gatekeeper of glomerular filtration barrier (GFB). As a major contributor to proteinuria, podocyte injury underlies a variety of glomerular diseases and becomes the challenge to patients and their families in general. At present, the therapeutic methods of podocyte injury mainly include angiotensin-converting enzyme inhibitors or angiotensin receptor blockers, steroid and immunosuppressive medications. Nevertheless, the higher cost and side effects seriously disturb patients with podocyte injury. Promisingly, traditional Chinese medicine (TCM) has received an increasing amount of attention from different countries in the treatment of podocyte injury by invigorating spleen and kidney, clearing heat and eliminating dampness, as well enriching qi and activating blood. Therefore, we searched articles published in peer-reviewed English-language journals through Google Scholar, PubMed, Web of Science, and Science Direct. The protective effects of active ingredients, herbs, compound prescriptions, acupuncture and moxibustion for treatment of podocyte injury were further summarized and analyzed. Meanwhile, we discussed feasible directions for future development, and analyzed existing deficiencies and shortcomings of TCM in the treatment of podocyte injury. In conclusion, this paper shows that TCM treatments can serve as promising auxiliary therapeutic methods for the treatment of podocyte injury.

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Baicalin alleviates adriamycin-induced focal segmental glomerulosclerosis and proteinuria by inhibiting the Notch1-Snail axis mediated podocyte EMT

Cited by 16 publications

References 31 publications

Nuclear paraspeckle assembly transcript 1 promotes the podocyte injury via targeting miR-23b-3p/B-cell lymphoma-2 interacting protein 3 like axis

Nuclear paraspeckle assembly transcript 1 promotes the podocyte injury via targeting miR-23b-3p/B-cell lymphoma-2 interacting protein 3 like axis

Flavonoids in Treatment of Chronic Kidney Disease

Recent Advances in Traditional Chinese Medicine for Treatment of Podocyte Injury

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