Baicalein pretreatment protects against liver ischemia/reperfusion injury via inhibition of NF-κB pathway in mice

Liu, Anding; Huang, Liang; Fan, Hua; Fang, Haoshu; Yang, Yan; Liu, Shenpei; Hu, Jifa; Hu, Qi; Dirsch, Olaf; Dahmen, Uta

doi:10.1016/j.intimp.2014.11.014

Cited by 46 publications

(52 citation statements)

References 30 publications

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“…Real time PCR for collagen-1, CTGF, TGF-β and mitochondrial DNA(mtDNA) was performed as described previously [19,20]. The sequences of primers are as follows: Collagen-1 forward: 5′-TGGCCTTGGAGGAAACTTTG-3′; reverse: 5′-CTTGGAAACCTTGTGGACC AG-3′, CTGF forward: 5′-ACTATGATGCGAGCCAACTGC-3′; reverse: 5′-TGTCCGGATGCACTTTTTGC-3′, TGF-β forward: 5′-TCTACAACCAACACAA CCCGG-3′; reverse: 5′-GAGCGCACAATCATGTTGGAC-3′, mtDNA specific forward: 5′-CCGCAAGGGAAAGATGAAAGAC-3′; reverse:5′-TCGTTTGGTTTCGGGGTTTC-3′ and β-actin forward: 5′-TGCACCACC AACTGCTTAG-3′; reverse: 5′-GGATGCAGGGATGATGTTC-3′.…”

Section: Quantitative Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Puerarin protects against CCl4-induced liver fibrosis in mice: possible role of PARP-1 inhibition

Wang

Shi

Feng

et al. 2016

International Immunopharmacology

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Section: Quantitative Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Puerarin protects against CCl4-induced liver fibrosis in mice: possible role of PARP-1 inhibition

Wang

Shi

Feng

et al. 2016

International Immunopharmacology

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“…In addition, some studies have demonstrated the effects of baicalein on cisplatin sensitivity via different pathways in various cancer cells . Baicalein has also exhibited a wide range of anti‐inflammatory effects associated with airway injury, liver injury and rheumatoid arthritis . In summary, baicalein has the potential to become an ideal adjuvant therapy in the treatment of cancer.…”

Section: Introductionmentioning

confidence: 99%

Baicalein inhibits cell growth and increases cisplatin sensitivity of A549 and H460 cells via miR‐424‐3p and targeting PTEN/PI3K/Akt pathway

Wang

Chen

et al. 2018

J Cellular Molecular Medi

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Lung cancer is the leading cause of death in individuals with malignant disease. Non‐small‐cell lung cancer (NSCLC) is the most common type of lung cancer, and chemotherapy drugs such as cisplatin are the most widely used treatment for this disease. Baicalein is a purified flavonoid compound that has been reported to inhibit cancer cell growth and metastasis and increase sensitization to chemotherapeutic drugs via different pathways. Therefore, we assessed the effects of baicalein on the proliferation, apoptosis and cisplatin sensitivity in the NSCLC A549 and H460 cell lines and determined the pathways through which baicalein exerts its effects. Baicalein was slightly toxic to normal human bronchial NHBE cells but inhibited growth, induced apoptosis and increased cisplatin sensitivity in A549 and H460 cells. Baicalein down‐regulated miR‐424‐3p, up‐regulated PTEN expression and down‐regulated expression of PI3K and p‐Akt in A549 and H460 cells. Dual‐luciferase reporter assay demonstrated that PTEN is a target gene of miR‐424‐3p, and overexpression of miR‐424‐3p or silencing of PTEN partially attenuated the effects of baicalein on A549 and H460 cells. Taken together, we concluded that baicalein inhibits cell growth and increases cisplatin sensitivity to A549 and H460 cells via down‐regulation of miR‐424‐3p and targeting the PTEN/PI3K/Akt pathway.

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“…Current research on the treatment of hepatic IR injury is largely centered on the use of anti-oxidants to prevent excess ROS formation [9][10][11]. However, these anti-oxidant agents do not preferentially target the liver and need to be administered several times in large doses to have a clinically significant effect.…”

Section: Introductionmentioning

confidence: 99%

Prophylactic Treatment with Cerium Oxide Nanoparticles Attenuate Hepatic Ischemia Reperfusion Injury in Sprague Dawley Rats

Manne¹,

Arvapalli

Graffeo³

et al. 2017

Cell Physiol Biochem

554

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Background: Hepatic ischemia reperfusion is one the main causes for graft failure following transplantation. Although, the molecular events that lead to hepatic failure following ischemia reperfusion (IR) are diverse and complex, previous studies have shown that excessive formation of reactive oxygen species (ROS) are responsible for hepatic IR injury. Cerium oxide (CeO 2 ) nanoparticles have been previously shown to act as an anti-oxidant and anti-inflammatory agent. Here, we evaluated the protective effects of CeO 2 nanoparticles on hepatic ischemia reperfusion injury. Methods: Male Sprague Dawley rats were randomly assigned to one of the four groups: Control, CeO 2 nanoparticle only, hepatic ischemia reperfusion (IR) group and hepatic ischemia reperfusion (IR) plus CeO 2 nanoparticle group (IR+ CeO 2 ). Partial warm hepatic ischemia was induced in left lateral and median lobes for 1h, followed by 6h of reperfusion. Animals were sacrificed after 6h of reperfusion and blood and tissue samples were collected and processed for various biochemical experiments. Results: Prophylactic treatment with CeO 2 nanoparticles (0.5mg/kg i.v (IR+CeO 2 group)) 1 hour prior to hepatic ischemia and subsequent reperfusion injury lead to a decrease in serum levels of alanine aminotransaminase and lactate dehydrogenase at 6 hours after reperfusion. These changes were accompanied by significant decrease in hepatocyte necrosis along with reduction in several serum inflammatory markers such as macrophage derived chemokine, macrophage inflammatory protein-2, KC/GRO, myoglobin and plasminogen activator inhibitor-1. However, immunoblotting demonstrated no significant changes in the levels of apoptosis related protein markers such as bax, bcl2 and caspase 3 in IR and IR+ CeO2 groups at 6 hours suggesting necrosis as the main pathway for hepatocyte death. Conclusion: Taken together, these data suggest that CeO 2 nanoparticles attenuate IR induced cell death and can be used as a prophylactic agent to prevent hepatic injury associated with graft failure.Room 315,

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Baicalein pretreatment protects against liver ischemia/reperfusion injury via inhibition of NF-κB pathway in mice

Cited by 46 publications

References 30 publications

Puerarin protects against CCl4-induced liver fibrosis in mice: possible role of PARP-1 inhibition

Puerarin protects against CCl4-induced liver fibrosis in mice: possible role of PARP-1 inhibition

Baicalein inhibits cell growth and increases cisplatin sensitivity of A549 and H460 cells via miR‐424‐3p and targeting PTEN/PI3K/Akt pathway

Prophylactic Treatment with Cerium Oxide Nanoparticles Attenuate Hepatic Ischemia Reperfusion Injury in Sprague Dawley Rats

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