Baicalein inhibits proliferation and collagen synthesis of mice fibroblast cell line NIH/3T3 by regulation of miR-9/insulin-like growth factor-1 axis

Abstract

The aberrant scar is a challenging problem. Baicalein has effects in attenuating hypertrophic scar formation. Herein, the roles of baicalein in NIH-3T3 were obtained. Cells were treated by baicalein. CCK-8 method and western blot were used to detect cell viability and proliferation-related factors. Furthermore, collagen 1, collagen 3 and a-SMA expression were detected by qRT-PCR and western blot. Besides, total soluble collagen was detected by Sircol assay. In addition, the levels of NF-jB and Wnt/b-catenin signal pathways related factors were determined by western blot. The relationship between miR-9 and insulin-like growth factor (IGF)-1 were tested by luciferase reporter assay, qRT-PCR and western blot. We found baicalein cell restrained proliferation and collagen production. Besides, baicalein increased expression of miR-9 and further experiments validated that transfection with miR-9 inhibitor reversed the results led by baicalein. Moreover, baicalein decreased the phosphorylation of pathway-related proteins while transfection with miR-9 inhibitor revised this result. Otherwise, IGF-1 was authenticated as a target of miR-9 and si-IGF-1 reversed the miR-9 inhibitor-induced change in cell proliferation and collagen production. In conclusion, baicalein restrained cell proliferation and collagen production by regulating miR-9/IGF-1 axis through NF-jB and Wnt/b-catenin signal pathways. HIGHLIGHTS:1. Baicalein restrains NIH/3T3 cell proliferation and collagen production. 2. Baicalein restrains NF-jB and Wnt/b-catenin signal pathways. 3. IGF-1 is a target of miR-9. 4. Baicalein exerts its functions by regulating miR-9/IGF-1 axis. ARTICLE HISTORY

Natural Baicalein-Rich Fraction as Radiosensitizer in Combination with Bismuth Oxide Nanoparticles and Cisplatin for Clinical Radiotherapy

“…13 , 78 Comparable to the present study, the pathways involved might include the increment of p53 genes and the decrease of cyclinD1, vascular endothelial growth factor (VEGF), CDK4 genes, and collagen-synthesis-related proteins such as α-smooth muscle actin, collagen-1, and collagen-3 gene expressions. 78 The mechanisms might also happen in the present study, which determined the low toxicity effect of the BRF. The cytoselectivity of an agent towards different cells is an excellent characteristic of a potent anticancer agent.…”

“…[75][76][77] BRF or baicalein compound would not disturb the NIH/3T3 cells' proliferation. 13,78 Comparable to the present study, the pathways involved might include the increment of p53 genes and the decrease of cyclinD1, vascular endothelial growth factor (VEGF), CDK4 genes, and collagen-synthesis-related proteins such as α-smooth muscle actin, collagen-1, and collagen-3 gene expressions. 78 The mechanisms might also happen in the present study, which determined the low toxicity effect of the BRF.…”

Hydroponically Grown Sanguisorba minor Scop.: Effects of Cut and Storage on Fresh-Cut Produce

“…Sanguisorba minor showed a complex secondary metabolite profile that was influenced by the cut. Flavonoid content increased in leaves obtained from C2, especially the sub-class of flavones, which is characterized by anti-mutagenic, antioxidant, anti-inflammatory, anti-viral and purgative effects and is also highly important in cancer prevention [33][34][35][36][37][38][39]. Moreover, secondary metabolites were downregulated in leaves from C2 as compared to those detected in leaves from C1, as evidenced by the combination of the VIP score (VIP > 1.3) and the fold-change (FC > 2).…”

“…Apigenin has also demonstrated other positive features for human health, such as the reduction of plasma levels of low-density lipoproteins and the inhibition of platelet aggregation, thus, its presence in the diet could be very important [33][34][35]. Other studies have shown that baicalein can mitigate cell proliferation and diminish the production of collagen; also, it possesses multiple effective properties for the treatment of different diseases including cancer and injuries [36][37][38][39]. Lastly, 7,4'-dihydroxyflavone, also named 5-deoxyluteolin, has shown impressive antimycobacterial activity and its anti-inflammatory activity is under evaluation [40].…”

Traditional Chinese medicine for hypertrophic scars—A review of the therapeutic methods and potential effects

“…The mechanism of the inhibitory effect of baicalin on HS was related to the TGF-β/Smad2/3 signaling pathways ( Zhang Y. F. et al, 2016 ). Also, baicalin regulates the miR-9/IGF-1 axis to inhibit cell proliferation and collagen production through NF-κB and Wnt/β-catenin signaling pathways ( Yang L. et al, 2019 ).…”