BaeR protein from Salmonella enterica serovar Paratyphi A induces inflammatory response in murine and human cell lines

Lee, Seung Jin; Awji, Elias Gebru; Kim, Myung Hee; Park, Seung Chun

doi:10.1016/j.micinf.2013.09.001

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…2 Salmonella causes four major human diseases, including enteric fever, gastroenteritis, bacteremia, and chronic asymptomatic carriage. 3,4 Notably, salmonellosis represents a major global health issue, with 21 million cases of typhoid fever and 1.3 million cases of gastroenteritis estimated per year. 5,6 Outbreaks are commonly associated with eggs, raw milk, fruits, vegetables, and poultry (as well as other meats).…”

Section: Introductionmentioning

confidence: 99%

Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms

Thavanathan¹,

Huang²,

Thong³

2015

IJN

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We have developed a colorimetric biosensor using a dual platform of gold nanoparticles and graphene oxide sheets for the detection of Salmonella enterica . The presence of the invA gene in S. enterica causes a change in color of the biosensor from its original pinkish-red to a light purplish solution. This occurs through the aggregation of the primary gold nanoparticles–conjugated DNA probe onto the surface of the secondary graphene oxide–conjugated DNA probe through DNA hybridization with the targeted DNA sequence. Spectrophotometry analysis showed a shift in wavelength from 525 nm to 600 nm with 1 μM of DNA target. Specificity testing revealed that the biosensor was able to detect various serovars of the S. enterica while no color change was observed with the other bacterial species. Sensitivity testing revealed the limit of detection was at 1 nM of DNA target. This proves the effectiveness of the biosensor in the detection of S. enterica through DNA hybridization.

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Section: Introductionmentioning

confidence: 99%

Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms

Thavanathan¹,

Huang²,

Thong³

2015

IJN

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Repurposing FDA-approved drugs for combating tigecycline resistance in Acinetobacter baumannii: in silico screening against BaeR protein

Alagesan,

Nagarajan,

Jeyakanthan

2024

Mol Divers

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BaeR protein acts as an activator of nuclear factor-kappa B and Janus kinase 2 to induce inflammation in murine cell lines

et al. 2016

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BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to induce an inflammatory response. BaeR protein was purified from Salmonella enterica Paratyphi A and subcloned into a pPosKJ expression vector. RAW 264.7 cells were treated with BaeR, and RNA was extracted by TRIzol reagent for RT-PCR. Cytokine gene expression was analyzed by using the comparative cycle threshold method, while western blotting and ELISA were used to assess protein expression. We confirmed that BaeR activates nuclear factor-kappa B (NF-κB), thereby inducing an inflammatory response and increases the production of interleukins (IL-)1β and IL-6. During this process, the Janus kinase 2 (JAK2)-STAT1 signaling pathway was activated, resulting in an increase in the release of interferons I and II. Additionally, COX-2 was activated and its expression increased with time. In conclusion, BaeR induced an inflammatory response through activation of NF-κB in addition to the MAPKs. Furthermore, activation of the JAK2-STAT1 pathway and COX-2 facilitated the cytokine binding activity, suggesting an additional role for BaeR in the modulation of the immune system of the host and the virulence activity of the pathogen.

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BaeR protein from Salmonella enterica serovar Paratyphi A induces inflammatory response in murine and human cell lines

Cited by 3 publications

References 34 publications

Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms

Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms

Repurposing FDA-approved drugs for combating tigecycline resistance in Acinetobacter baumannii: in silico screening against BaeR protein

BaeR protein acts as an activator of nuclear factor-kappa B and Janus kinase 2 to induce inflammation in murine cell lines

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