Baculoviruses: Sophisticated Pathogens of Insects

Clem, Rollie J.; Passarelli, A. Lorena

doi:10.1371/journal.ppat.1003729

Cited by 108 publications

(85 citation statements)

References 19 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…UDP-glucuronosyltransferases (UGTs) are a major class of phase II drug metabolizing enzymes that play an important role in detoxifying a large number of xenobiotics through conjugation reactions [28]. Ecdysteroid UGT prevents larval molting by inactivating the ecdysone by catalyzing the transfer of glucose from UDP-glucose to ecdysteroids, the major insect molting hormones [29,30]. In this study, UGT transcripts were increased in the SR strain in response to spirotetramat exposure, which may be responsible for the extensive suppression of cuticle protein expression (Table 4).…”

Section: Expression Profiling Analysis Between Sr and Ss Transcriptommentioning

confidence: 99%

Spirotetramat resistance adaption analysis of Aphis gossypii Glover by transcriptomic survey

Pan

Yang

Gao

et al. 2015

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

Section: Expression Profiling Analysis Between Sr and Ss Transcriptommentioning

confidence: 99%

Spirotetramat resistance adaption analysis of Aphis gossypii Glover by transcriptomic survey

Pan

Yang

Gao

et al. 2015

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

“…This type of pathogen screening can be especially useful for helping inform conservation decision-making. By contrast, testing for acute infections, such as baculoviruses, which inflict the immature stages of insects that can be particularly devastating for captive colonies of Lepidoptera serve only to confirm disease presence [54]. While useful for potentially directing changes to husbandry protocols to minimize initial infection and spread, effective treatment of afflicted organisms is unlikely.…”

Section: Increase Use Of Genetic and Health Screeningmentioning

confidence: 99%

Improving Standards for At-Risk Butterfly Translocations

2018

View full text Add to dashboard Cite

Abstract:The use of human mediated translocations has been an increasing component of many species recovery initiatives, including for numerous imperiled Lepidopteran species. Despite the identified need for this ex situ strategy, few such programs are conducted in a scientifically repeatable way, are executed with a structured decision-making process, are well documented throughout, or are documented only in gray literature. The International Union for Conservation of Nature's Guidelines for Reintroductions and Other Conservation Translocations are an important tool for conservation practitioners to help implement comprehensive translocation planning. These generalized guidelines are intended to be applicable to all taxa. Though there is a growing body of literature and supplementary guidelines for many vertebrate classes, other proposed standards fail to capture the specific biology of many invertebrate groups, like Lepidoptera. Here, we present a targeted list of detailed recommendations that are appropriate for Lepidopteran translocation programs to expand on the broad and tested guidelines developed by the IUCN. We assert that the increased standardization and repeatability among Lepidopteran translocations will improve the conservation outcomes.

show abstract

“…However, infectious agents have evolved mechanisms to overcome or even manipulate this hostile environment in order to survive and reproduce (Clem & Passarelli, 2013;Ferrandon et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of hesp018, a baculovirus-encoded serpin gene

Ardisson-Araújo

Rohrmann

Ribeiro

et al. 2015

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

The serpin family of serine proteinase inhibitors plays key roles in a variety of biochemical pathways. In insects, one of the important functions carried out by serpins is regulation of the phenoloxidase (PO) cascade -a pathway that produces melanin and other compounds that are important in insect humoral immunity. Recent sequencing of the baculovirus Hemileuca sp. nucleopolyhedrovirus (HespNPV) genome revealed the presence of a gene, hesp018, with homology to insect serpins. To our knowledge, hesp018 is the first viral serpin homologue to be characterized outside of the chordopoxviruses. The Hesp018 protein was found to be a functional serpin with inhibitory activity against a subset of serine proteinases. Hesp018 also inhibited PO activation when mixed with lepidopteran haemolymph. The Hesp018 protein was secreted when expressed in lepidopteran cells and a baculovirus expressing Hesp018 exhibited accelerated production of viral progeny during in vitro infection. Expression of Hesp018 also reduced caspase activity induced by baculovirus infection, but caused increased cathepsin activity. In infected insect larvae, expression of Hesp018 resulted in faster larval melanization, consistent with increased activity of viral cathepsin. Finally, expression of Hesp018 increased the virulence of a prototype baculovirus by fourfold in orally infected neonate Trichoplusia ni larvae. Based on our observations, we hypothesize that hesp018 may have been retained in HespNPV due to its ability to inhibit the activity of select host proteinases, possibly including proteinases involved in the PO response, during infection of host insects.

show abstract

Baculoviruses: Sophisticated Pathogens of Insects

Cited by 108 publications

References 19 publications

Spirotetramat resistance adaption analysis of Aphis gossypii Glover by transcriptomic survey

Spirotetramat resistance adaption analysis of Aphis gossypii Glover by transcriptomic survey

Improving Standards for At-Risk Butterfly Translocations

Functional characterization of hesp018, a baculovirus-encoded serpin gene

Contact Info

Product

Resources

About