Baculovirus expression of beak and feather disease virus (BFDV) capsid protein capable of self-assembly and haemagglutination

Stewart, Meredith; Bonne, Nicolai; Shearer, Patrick; Khalesi, Bahman; Sharp, Margaret; Raidal, Shane R.

doi:10.1016/j.jviromet.2006.12.011

Cited by 17 publications

(5 citation statements)

References 38 publications

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“…Immunohistochemical staining. Immunohistochemical staining using primary monoclonal antibodies to recombinant BFDV capsid protein (Stewart et al, 2007 and a horseradish-peroxidase-conjugated secondary antibody were performed on tissue sections from case 05-106. Briefly, 5 mm sections of formalin-fixed and paraffin-embedded feather tissue were cut using a Leica RM 2135 microtome, placed onto glass slides, de-waxed three times in xylene for 3 min and re-hydrated using decreasing ethanol concentrations and a final wash in Tris buffer for 3 min.…”

Section: Methodsmentioning

confidence: 99%

Beak and feather disease virus infection in cockatiels (Nymphicus hollandicus)

et al. 2008

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Psittacine beak and feather disease is known to occur in a wide range of psittacine species; however, there are no scientific or credible anecdotal reports of psittacine beak and feather disease occurring in the cockatiel (Nymphicus hollandicus) despite it being one of the world's most commonly kept companion bird species. Consequently, this has resulted in speculation that the species may have some innate resistance to beak and feather disease virus (BFDV) infection. To investigate this hypothesis we conducted a survey of cockatiels (n=88) at commercial aviaries to investigate whether BFDV infection occurs in cockatiels, and found that all birds were virus-free by polymerase chain reaction and haemagglutination assay and had no detectable antibody titre by haemagglutination-inhibition assay. In addition to this, we sequenced the genome of two BFDV isolates obtained from diseased cockatiel feathers and performed cross-reactivity assays using virus eluted from these feathers and sera from naturally immune psittacine birds. Serological cross-reactivity results and phylogenetic analysis of the nucleotide sequences indicated that the cockatiel virus isolates were serologically and genetically different to other BFDV isolates. This is the first paper to report evidence of an antigenically distinct BFDV in psittacine birds.

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Section: Methodsmentioning

confidence: 99%

Beak and feather disease virus infection in cockatiels (Nymphicus hollandicus)

et al. 2008

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“…Recombinant BFDV CP expressed in insect cell culture spontaneously assembles into particles ranging in size from 16 to 22 nm in diameter, which falls within the size range reported for infectious virions (10–22 nm in diameter) [ 41 ]. Attempts to produced BFDV VLPs using other recombinant systems have been mixed: for example, expression of the full-length BFDV CP using bacterial fermentation resulted in insoluble protein and low yields [ 15 , 27 ].…”

Section: Introductionmentioning

confidence: 57%

“…This description perfectly fits the infectious BFDV particles seen by Crowther , et al [ 20 ]. The plant-produced BFDV particles ranged between 13 and 23 nm in diameter, and were similar to putative BFDV VLPs produced in insect cell cultures, which ranged between 16 and 22 nm diameter [ 41 ]. The predominant diameter measured was approximately 17 nm, which falls neatly within the 10–22 nm range reported for infectious virions, and is the same diameter as has been reported for the solved atomic structure of BFDV VLPs [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

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Recombinant expression of beak and feather disease virus capsid protein and assembly of virus-like particles in Nicotiana benthamiana

Regnard

Rybicki

Hitzeroth

2017

Virol J

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BackgroundBeak and feather disease virus (BFDV) is an important disease causing agent affecting psittacines. BFDV is highly infectious and can present as acute, chronic or subclinical disease. The virus causes immunodeficiency and is often associated with secondary infections. No commercial vaccine is available and yields of recombinant BFDV capsid protein (CP) expressed in insect cells and bacteria are yet to be seen as commercially viable, although both systems produced BFDV CP that could successfully assemble into virus-like particles (VLPs). Plants as expression systems are increasingly becoming favourable for the production of region-specific and niche market products. The aim of this study was to investigate the formation and potential for purification of BFDV VLPs in Nicotiana benthamiana.MethodsThe BFDV CP was transiently expressed in N. benthamiana using an Agrobacterium-mediated system and plant expression vectors that included a bean yellow dwarf virus (BeYDV)-based replicating DNA vector. Plant-produced BFDV CP was detected using immunoblotting. VLPs were purified using sucrose cushion and CsCl density gradient centrifugation and visualised using transmission electron microscopy.ResultsIn this study we demonstrate that the BFDV CP can be successfully expressed in N. benthamiana, albeit at relatively low yield. Using a purification strategy based on centrifugation we demonstrated that the expressed CP can self-assemble into VLPs that can be detected using electron microscopy. These plant-produced BFDV VLPs resemble those produced in established recombinant expression systems and infectious virions. It is possible that the VLPs are spontaneously incorporating amplicon DNA produced from the replicating BeYDV plant vector.ConclusionsThis is the first report of plant-made full-length BFDV CP assembling into VLPs. The putative pseudovirions could be used to further the efficacy of vaccines against BFDV.

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“…There are three related porcine circoviruses (PCV-1, -2 and -3) causing serious disease in swine, and a variety of avian-infecting viruses, with the most important being beak and feather disease virus (BFDV) of psittacines (Ritchie et al, 1989;Shulman & Davidson, 2017). Expression of the CPs of both viruses in insect cells or in E. coli can result in VLP formation (Kim, Kim, Kang, & Lyoo, 2002;Sarker et al, 2015;Stewart et al, 2007;Xi et al, 2016), and a commercial vaccine against PCV-2 is made in insect cells (Martelli et al, 2011). PCVs are culturable, while BFDVs are not, which complicates making or even testing conventional vaccines for the latter.…”

Section: Circovirusesmentioning

confidence: 99%

Plant molecular farming of virus‐like nanoparticles as vaccines and reagents

Rybicki

2019

WIREs Nanomed Nanobiotechnol

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The use of plants for the production of virus‐like nanoparticles (VNPs) dates back to separating natural empty capsids of plant viruses from whole virions nearly 70 years ago, through to the present use of transgenic plants or recombinant Agrobacterium tumefaciens and/or plant virus‐derived vectors for the transient expression of engineered viral or other structural proteins in plants—a production system also known as molecular farming. Plant production of heterologous proteins has major advantages in terms of convenience—whole plants are generally used, and processes do not need to be sterile—and cost, as bulk biomass production is significantly cheaper than by any other method. Plant‐made VNPs in current use for nanotechnology include whole virions and naturally occurring empty capsids of plant viruses, and particles made by reassembly of coat protein (CP) purified from virions or by recombinant expression. Engineered VNP‐forming animal or human virus CPs expressed in plants include L1 protein from human papillomaviruses, human norovirus CP, hepatitis B surface and core antigens, influenza virus HA protein and HIV Gag polyprotein forming large enveloped particles by budding, orbi‐ and rotavirus particles that require assembly of four co‐expressed proteins, and polio‐ and foot and mouth disease viruses which require proteolytic processing of a polyprotein precursor to form 4‐component VNPs. Both plant and animal virus‐derived plant‐made VNPs can be used for surface and internal display of heterologous peptides or even whole proteins. A significant recent development has been the production of pseudovirions in plants, comprising plant or animal virus CPs and RNA or DNA pseudogenomes that can be used to deliver nucleic acid payloads into cultured cells or specific tissues or tumors in whole animals. This article is characterized under: Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures Therapeutic Approaches and Drug Discovery > Emerging Technologies Diagnostic Tools > in vivo Nanodiagnostics and Imaging

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Baculovirus expression of beak and feather disease virus (BFDV) capsid protein capable of self-assembly and haemagglutination

Cited by 17 publications

References 38 publications

Beak and feather disease virus infection in cockatiels (Nymphicus hollandicus)

Beak and feather disease virus infection in cockatiels (Nymphicus hollandicus)

Recombinant expression of beak and feather disease virus capsid protein and assembly of virus-like particles in Nicotiana benthamiana

Plant molecular farming of virus‐like nanoparticles as vaccines and reagents

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