Baculovirus-Encoded MicroRNAs: A Brief Overview and Future Prospects

Tang, Qi; Qiu, Lipeng; Li, Guohui

doi:10.1007/s00284-018-1443-y

Cited by 17 publications

(7 citation statements)

References 53 publications

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“…Whether the reduction in PLP expression after SeM-NPV infection is the result of the direct effect of the virus on the host, a defense response of the host, or just a side effect of the physiological changes associated to the infection, remains to be elucidated. Downregulation of host genes to increase viral fitness or evade the host's immune response by viral encoded miRNAs has been described for several baculovirus species (Singh et al, 2010;Kharbanda et al, 2015;Tang et al, 2019). At present, our analysis of SeMNPV-expressed miRNAs has not revealed miRNAs targeting to Se-PLP (data not shown).…”

Section: Discussionmentioning

confidence: 64%

A proctolin‐like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion

Llopis-Giménez

Han

et al. 2021

Insect Science

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Baculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues, included the central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein-like molecules functioning as neurohormones, neurotransmitters, or neuromodulators. These peptides are involved in regulating animal physiology and behavior and could be altered after baculovirus infection. In this study, we have investigated the effect of Spodoptera exigua multiple nucleopolyhedrovirus (SeM-NPV) infection on expression of Spodoptera exigua neuropeptides and neuropeptide-like genes. Expression of the gene encoding a polypeptide that resembles the well-known insect neuropeptide proctolin and named as proctolin-like peptide (PLP), was downregulated in the larval brain following infection and was chosen for further analysis. A recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) overexpressing the Cterminal part of the PLP was generated and used in bioassays using S. exigua larvae to study its influence on the viral infection and insect behavior. AcMNPV-PLP-infected larvae showed less locomotion activity and a reduction in growth compared to larvae infected with wild type AcMNPV or mock-infected larvae. These results are indicative of this new peptide as a neuromodulator that regulates visceral and skeletal muscle contractions and offers a novel effector involved in the behavioral changes during baculovirus infection.

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Section: Discussionmentioning

confidence: 64%

A proctolin‐like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion

Llopis-Giménez

Han

et al. 2021

Insect Science

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“…Whether the reduction in proctolin expression after SeMNPV infection is the result of the direct effect of the virus on the host, a defence response of the host, or just a side effect of the physiological changes associated to the infection, remain to be elucidated. Down-regulation of host genes to increase viral fitness or evade the host's immune response by viral encoded miRNAs has been described for several baculovirus species (Kharbanda et al, 2015;Singh et al, 2010;Tang et al, 2019). At present, our analysis of SeMNPV-expressed miRNAs has not revealed miRNAs targeting to SeProctolin (data not shown).…”

Section: Gene Expression Analysis Of Larval Brain Revealed a Clear Domentioning

confidence: 64%

Neuropeptidome regulation after baculovirus infection. A focus on proctolin and its relevance in locomotion and digestion

Llopis-Giménez

Han

et al. 2020

Preprint

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Baculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues. Some behavioral and physiological changes in lepidopteran larvae have been described following a baculovirus infection and those changes could be connected with alterations in the host’s central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein-like molecules functioning as neurohormones, neurotransmitters or neuromodulators. These peptides are involved in regulating animal physiology and behaviour and could also be targeted by baculoviruses in order to achieve host behavioural manipulations leading to increased viral fitness. In this study, we have investigated the effect of a Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) infection on the neuropeptidome gene expression of Spodoptera exigua larval heads and brains. Expression of the gene encoding the neuropeptide proctolin was severely downregulated following infection and was chosen for further analysis. A recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) overexpressing the S. exigua proctolin gene was generated and used in bioassays using S. exigua larvae to study its influence on the viral infection. AcMNPV-proctolin infected larvae showed less locomotion activity and suffered a loss of weight compared to larvae infected with wild type AcMNPV or mock-infected larvae. These results provide additional information on the role of proctolin during a baculovirus infection, and offers a novel hypothesis for the molecular bases for the behavioral changes associated with a baculovirus infection.

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“…A huge amount of literature is available describing miRNAs' identification from insect hosts, but the exact role in host-pathogen interactions is still limited to a few reports (Ylla et al, 2016;Kozomara et al, 2019). Moreover, these studies mainly focus on miRNA alterations due to the viral attack in insects (Tang et al, 2019).…”

Section: Viral Mirna Can Target Viral Own Genesmentioning

confidence: 99%

MicroRNA-Mediated Host-Pathogen Interactions Between Bombyx mori and Viruses

2021

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MicroRNAs (miRNAs), small non-coding RNAs of about 22 nucleotides, have been reported to regulate gene expression at the posttranscriptional level and are involved in several biological processes such as immunity, development, metabolism, and host-pathogen interactions. Apart from miRNAs encoded by the host, miRNAs produced by pathogens also regulate host genes to facilitate virus replication and evasion of the host defense responses. In recent years, accumulated studies suggest that viral infections alter the host miRNAs expression profile, and both cellular and viral miRNAs may play vital roles in host-pathogen interactions. Bombyx mori, one of the critical lepidopteran model species, is an economically important insect for silk production. The mechanism of interaction between B. mori and its pathogens and their regulation by miRNAs has been extensively studied. Therefore, in this review, we aim to highlight the recent information and understanding of the virus-encoding miRNAs and their functions in modulating viral and host (B. mori) genes. Additionally, the response of B. mori derived miRNAs to viral infection is also discussed. A detailed critical view about miRNAs’ regulatory roles in B. mori-virus interactions will help us understand molecular networks and develop a sustainable antiviral strategy.

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Baculovirus-Encoded MicroRNAs: A Brief Overview and Future Prospects

Cited by 17 publications

References 53 publications

A proctolin‐like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion

A proctolin‐like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion

Neuropeptidome regulation after baculovirus infection. A focus on proctolin and its relevance in locomotion and digestion

MicroRNA-Mediated Host-Pathogen Interactions Between Bombyx mori and Viruses

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