Baculovirus-Derived Vectors for Immunization and Therapeutic Applications

Fabre, María Laura; Arrías, Paula Nazarena; Masson, Tomás; Pidre, Matías L.; Romanowski, Vı́ctor

doi:10.1016/b978-0-12-814966-9.00011-1

Cited by 10 publications

(11 citation statements)

References 160 publications

(101 reference statements)

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“…BEVS offers a eukaryotic environment providing adequate posttranslational modification, but the insects are not capable of producing human-type N-glycoproteins (Fabre et al 2020). Since 1983, when the first protein was expressed by means of this system, different strategies have been implemented in order to improve the technology, upon considering both the host and the viral vector (Martínez-Solís et al 2019).…”

Section: Baculoviruses As Tools For the Control And Diagnosis Of Emerging Human Infectious Diseasesmentioning

confidence: 99%

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

Targovnik

Simonin

Callum

et al. 2021

Appl Microbiol Biotechnol

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Baculoviruses are insect pathogens widely used as biotechnological tools in different fields of life sciences and technologies. The particular biology of these entities (biosafety viruses 1; large circular double-stranded DNA genomes, infective per se; generally of narrow host range on insect larvae; many of the latter being pests in agriculture) and the availability of molecular-biology procedures (e.g., genetic engineering to edit their genomes) and cellular resources (availability of cell lines that grow under in vitro culture conditions) have enabled the application of baculoviruses as active ingredients in pest control, as systems for the expression of recombinant proteins (Baculovirus Expression Vector Systems-BEVS) and as viral vectors for gene delivery in mammals or to display antigenic proteins (Baculoviruses applied on mammals-BacMam). Accordingly, BEVS and BacMam technologies have been introduced in academia because of their availability as commercial systems and ease of use and have also reached the human pharmaceutical industry, as incomparable tools in the development of biological products such as diagnostic kits, vaccines, protein therapies, and-though still in the conceptual stage involving animal models-gene therapies. Among all the baculovirus species, the Autographa californica multiple nucleopolyhedrovirus has been the most highly exploited in the above utilities for the human-biotechnology field. This review highlights the main achievements (in their different stages of development) of the use of BEVS and BacMam technologies for the generation of products for infectious and noninfectious human diseases. Key points• Baculoviruses can assist as biotechnological tools in human health problems.• Vaccines and diagnosis reagents produced in the baculovirus platform are described.• The use of recombinant baculovirus for gene therapy-based treatment is reviewed.

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Section: Baculoviruses As Tools For the Control And Diagnosis Of Emerging Human Infectious Diseasesmentioning

confidence: 99%

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

Targovnik

Simonin

Callum

et al. 2021

Appl Microbiol Biotechnol

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“…The incorporation of exogenous proteins expressed by the cell line brings a new perspective to the use of OBs as carriers for biotechnological uses, including applications other than baculovirus-based biopesticides, such as OB-associated antigens and the use of polyhedra as slow delivery of bioactive polypeptides Fabre et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

A simplified strategy to package foreign proteins into baculovirus occlusion bodies without engineering the viral genome

Fabre

Masson

Haase

et al. 2020

Journal of Biotechnology

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“…Some mammalian promoters used to initiate gene transcription include Rous-sarcoma virus long terminal repeats (RSV-LTR), cytomegalovirus (CMV), simian virus 40 (SV40), chicken beta-actin (CAG), hepatitis B virus (HBV), human a-fetoprotein/ubiquitin C promoter, and drosophila heat shock protein 70 (hsp70) promoter. 27 Viral and mammalian promoters can be used in conjugation with genomic enhancers to promote transgene transcription. Specifically, the insertion of an additional homologous region 1 (hr1) into baculoviruses has been used to activate mammalian promoters and results in improved stability, overexpression of the transgene, and prolonged transgene expression.…”

Section: Baculoviruses As Gene Delivery Systemsmentioning

confidence: 99%

Baculoviruses in Gene Therapy and Personalized Medicine

Schaly¹,

Ghebretatios²,

Prakash³

2021

BTT

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This review will outline the role of baculoviruses in gene therapy and future potential in personalized medicine. Baculoviruses are a safe, non-toxic, non-integrative vector with a large cloning capacity. Baculoviruses are also a highly adaptable, low-cost vector with a broad tissue and host tropism due to their ability to infect both quiescent and proliferating cells. Moreover, they only replicate in insect cells, not mammalian cells, improving their biosafety. The beneficial properties of baculoviruses make it an attractive option for gene delivery. The use of baculoviruses in gene therapy has advanced significantly, contributing to vaccine production, anti-cancer therapies and regenerative medicine. Currently, baculoviruses are primarily used for recombinant protein production and vaccines. This review will also discuss methods to optimize baculoviruses protein production and mammalian cell entry, limitations and potential for gene therapy and personalized medicine. Limitations such as transient gene expression, complement activation and virus fragility are discussed in details as they can be overcome through further genetic modifications and other methods. This review concludes that baculoviruses are an excllent candidate for gene therapy, personalized medicine and other biotherapeutic applications.

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Baculovirus-Derived Vectors for Immunization and Therapeutic Applications

Cited by 10 publications

References 160 publications

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

A simplified strategy to package foreign proteins into baculovirus occlusion bodies without engineering the viral genome

Baculoviruses in Gene Therapy and Personalized Medicine

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