Baculovirus: an Insect-derived Vector for Diverse Gene Transfer Applications

Airenne, Kari J.; Hu, Yu‐Chen; Kost, Thomas A.; Smith, Richard H.; Kotin, Robert M.; Ono, Chikako; Matsuura, Yoshiharu; Wang, Shu; Ylä‐Herttuala, Seppo

doi:10.1038/mt.2012.286

Cited by 160 publications

(131 citation statements)

References 146 publications

(229 reference statements)

Supporting

Mentioning

125

Contrasting

Unclassified

Order By: Relevance

“…BV is an enveloped dsDNA virus that is pathogenic for insects but cannot replicate in mammals, although it can transduce them (35). We have previously established the strong adjuvant properties of BV, which promotes the in vivo maturation of DCs and the production of inflammatory mediators through mechanisms primarily mediated by IFNs-I, leading to potent humoral and CTL responses against a coadministered Ag (29).…”

Section: Bv Triggers Innate and Adaptive Immune Responses Through Tlrmentioning

confidence: 99%

Conventional but Not Plasmacytoid Dendritic Cells Foster the Systemic Virus–Induced Type I IFN Response Needed for Efficient CD8 T Cell Priming

Hervás-Stubbs

Riezu-Boj

Mancheño

et al. 2014

The Journal of Immunology

View full text Add to dashboard Cite

Plasmacytoid dendritic cells (pDCs) are considered to be the principal type-I IFN (IFN-I) source in response to viruses, whereas the contribution of conventional DCs (cDCs) has been underestimated because, on a per-cell basis, they are not considered professional IFN-I–producing cells. We have investigated their respective roles in the IFN-I response required for CTL activation. Using a nonreplicative virus, baculovirus, we show that despite the high IFN-I–producing abilities of pDCs, in vivo cDCs but not pDCs are the pivotal IFN-I producers upon viral injection, as demonstrated by selective pDC or cDC depletion. The pathway involved in the virus-triggered IFN-I response is dependent on TLR9/MyD88 in pDCs and on stimulator of IFN genes (STING) in cDCs. Importantly, STING is the key molecule for the systemic baculovirus-induced IFN-I response required for CTL priming. The supremacy of cDCs over pDCs in fostering the IFN-I response required for CTL activation was also verified in the lymphocytic choriomeningitis virus model, in which IFN-β promoter stimulator 1 plays the role of STING. However, when the TLR-independent virus-triggered IFN-I production is impaired, the pDC-induced IFNs-I have a primary impact on CTL activation, as shown by the detrimental effect of pDC depletion and IFN-I signaling blockade on the residual lymphocytic choriomeningitis virus–triggered CTL response detected in IFN-β promoter stimulator 1−/− mice. Our findings reveal that cDCs play a major role in the TLR-independent virus-triggered IFN-I production required for CTL priming, whereas pDC-induced IFNs-I are dispensable but become relevant when the TLR-independent IFN-I response is impaired.

show abstract

Section: Bv Triggers Innate and Adaptive Immune Responses Through Tlrmentioning

confidence: 99%

Conventional but Not Plasmacytoid Dendritic Cells Foster the Systemic Virus–Induced Type I IFN Response Needed for Efficient CD8 T Cell Priming

Hervás-Stubbs

Riezu-Boj

Mancheño

et al. 2014

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Baculoviruses not only infect insect cells, but can also transduce mammalian cells efficiently [15,[82][83][84]. By choosing mammalian-active promoters instead of polh, p10 or other baculoviral promoters, proteins of interest can be produced from a baculovirus that has entered a mammalian host cell.…”

Section: 2/ Multibac In Pharma and Biotechmentioning

confidence: 99%

“…Baculovirus is thus emerging as a highly promising gene delivery tool into mammalian cells, for a multitude of applications [14,15]. In this context, multigene MultiBac constructions were successfully used to produce recombinant adeno-associated viruses (AAVs) for gene therapy [12,13,40].…”

Section: 2/ Multibac In Pharma and Biotechmentioning

confidence: 99%

See 1 more Smart Citation

The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics

Sarı

Gupta

Raj

et al. 2016

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatment of human diseases. Recombinant production by the baculovirus / insect cell expression system is particularly useful for expressing proteins of eukaryotic origin and their complexes. MultiBac, an advanced baculovirus / insect cell system has been widely adopted in the last decade to produce multiprotein complexes with many subunits that were hitherto inaccessible, for academic and industrial research and development. The MultiBac system, its development and numerous applications are presented. Future opportunities for utilizing MultiBac to catalyze discovery are outlined.

show abstract

“…The other cell lines are used to host the baculovirus expression vector system (BEVS), which remains the gold standard in insect-based expression systems for recombinant proteins [8,9]. However, the BEVS is used not only for the production of recombinant proteins but also as a gene shuttle for stem cells [10] and for vaccine manufacturing [9].…”

Section: Introductionmentioning

confidence: 99%

The components of shear stress affecting insect cells used with the baculovirus expression vector system

Weidner

Druzinec

Mühlmann

et al. 2017

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

Insect-based expression platforms such as the baculovirus expression vector system (BEVS) are widely used for the laboratory-and industrial-scale production of recombinant proteins. Thereby, major drawbacks to gain high-quality proteins are the lytic infection cycle and the shear sensitivity of infected insect cells due to turbulence and aeration. Smaller bubbles were formerly assumed to be more harmful than larger ones, but we found that cell damage is also dependent on the concentration of protective agents such as Pluronic ® . At the appropriate concentration, Pluronic forms a layer around air bubbles and hinders the attachment of cells, thus limiting the damage. In this context, we used microaeration to vary bubble sizes and confirmed that size is not the most important factor, but the total gas surface area in the reactor is. If the surface area exceeds a certain threshold, the concentration of Pluronic is no longer sufficient for cell protection. To investigate the significance of shear forces, a second study was carried out in which infected insect cells were cultivated in a hollow fiber module to protect them from shear forces. Both model studies revealed important aspects of the design and scale-up of BEVS processes for the production of recombinant proteins.

show abstract

Baculovirus: an Insect-derived Vector for Diverse Gene Transfer Applications

Cited by 160 publications

References 146 publications

Conventional but Not Plasmacytoid Dendritic Cells Foster the Systemic Virus–Induced Type I IFN Response Needed for Efficient CD8 T Cell Priming

Conventional but Not Plasmacytoid Dendritic Cells Foster the Systemic Virus–Induced Type I IFN Response Needed for Efficient CD8 T Cell Priming

The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics

The components of shear stress affecting insect cells used with the baculovirus expression vector system

Contact Info

Product

Resources

About