Bacteroides spp. and traditional fecal indicator bacteria in water quality assessment – An integrated approach for hydric resources management in urban centers

Teixeira, Pedro; Dias, Deodália; Costa, Sílvia; Brown, Barbara E.; Silva, Susana; Valério, Elisabete

doi:10.1016/j.jenvman.2020.110989

Cited by 23 publications

(13 citation statements)

References 52 publications

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Section: Pcr Detection 1-fresh Coastal Sand Samplesmentioning

confidence: 99%

“…At the time of this contamination event, we had available and validated human and cow molecular markers from the previous work of Teixeira et al [54]. Following the DNA extraction (using Approach A) from fresh coastal sands, samples were tested by qPCR for human and cow contamination using the conditions previously described in Teixeira et al [54]. Briefly, for the HF183/Bac287 Real-Time qPCR assay, 10 µL reaction mixtures containing 1× SsoAdvanced Universal Probe Supermix (Biorad, 0.2 mg/mL BSA (bovine serum albumin), 0.5 µM of each primer, 80 nM (FAM)-labelled probe, and 2 µL of DNA template or molecular-grade water (notemplate control-NTC) were prepared.…”

Section: Pcr Detection 1-fresh Coastal Sand Samplesmentioning

confidence: 99%

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Microbial Source Tracking as a Method of Determination of Beach Sand Contamination

Valério

Santos

Teixeira

et al. 2022

IJERPH

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Beach sand may act as a reservoir for numerous microorganisms, including enteric pathogens. Several of these pathogens originate in human or animal feces, which may pose a public health risk. In August 2019, high levels of fecal indicator bacteria (FIB) were detected in the sand of the Azorean beach Prainha, Terceira Island, Portugal. Remediation measures were promptly implemented, including sand removal and the spraying of chlorine to restore the sand quality. To determine the source of the fecal contamination, during the first campaign, supratidal sand samples were collected from several sites along the beach, followed by microbial source tracking (MST) analyses of Bacteroides marker genes for five animal species, including humans. Some of the sampling sites revealed the presence of marker genes from dogs, seagulls, and ruminants. Making use of the information on biological sources originating partially from dogs, the municipality enforced restrictive measures for dog-walking at the beach. Subsequent sampling campaigns detected low FIB contamination due to the mitigation and remediation measures that were undertaken. This is the first case study where the MST approach was used to determine the contamination sources in the supratidal sand of a coastal beach. Our results show that MST can be an essential tool to determine sources of fecal contamination in the sand. This study shows the importance of holistic management of beaches that should go beyond water quality monitoring for FIB, putting forth evidence for beach sand monitoring.

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Section: Pcr Detection 1-fresh Coastal Sand Samplesmentioning

confidence: 99%

Section: Pcr Detection 1-fresh Coastal Sand Samplesmentioning

confidence: 99%

Microbial Source Tracking as a Method of Determination of Beach Sand Contamination

Valério

Santos

Teixeira

et al. 2022

IJERPH

Self Cite

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“…At the time of this contamination event, we had available and validated human and cow molecular markers from previous work [54]. Following the first DNA extraction method from sand, samples were tested by qPCR for human and cow contamination using the conditions previously described in [54].…”

Section: Pcr Detection 1 -Fresh Coastal Sand Samplesmentioning

confidence: 99%

Beach Sand Contaminated by Dog Walking? A Molecular Case Study

Valério¹,

Santos²,

Teixeira³

et al. 2022

Preprint

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Beach sand may act as a reservoir for numerous micro-organisms, including enteric pathogens. Several of these pathogens originate in human or animal feces, which may pose a public health risk. In August 2019, high levels of fecal indicator bacteria (FIB) were detected in the sand of the Azorean beach Prainha, Terceira Island, Portugal. Remediation measures were promptly implemented, including sand removal and the spraying of chlorine to restore the beach sand quality. To determine the biological source of the contamination, during the first campaign, supratidal sand samples were collected from several sites along the beach, followed by microbial source tracking (MST) analyses of Bacteroides markers for five animal species, including humans. Some of the sampling sites revealed the presence of marker genes from dogs, seagulls, and ruminants. Making use of the information on biological sources originating partially from dogs, the municipality enforced restrictive measures for dog-walking at the beach. Subsequent sampling campaigns detected low FIB contamination due to the mitigation and remediation measures that were undertaken, thereby no longer requiring MST marker-gene analysis. This is the first case study where the MST approach was used to determine the contamination sources in the supratidal sand of a coastal beach. Our results show that MST can be an essential approach to determine sources of fecal contamination in the sand. This study shows the importance of holistic management of beaches that should go beyond water quality monitoring for FIB, putting forth evidence for the need for sands also to be monitored.

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“…The identification of the presence of contaminating microorganisms (e.g., E. coli) or pathogenic microorganisms (e.g., Giardia spp., Cryptosporidium spp.) allows for limiting the spread of diseases potentially harmful to humans [56,57]. The presence of microbial water contamination is mainly linked to the spillage of civil and/or livestock sewage, incorrect water treatment, and the inability to disperse the microbial load of the receiving water body [58].…”

Section: (I) Chemical and Biological Water Pollutionmentioning

confidence: 99%

The Health of the Water Planet: Challenges and Opportunities in the Mediterranean Area. An Overview

Vinci

Maddaloni

Mancini

et al. 2021

Earth

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According to the United Nations (2020), since the 1980s, the global overall rate of water use has grown by 1% per year, and it is projected that, by 2050, humanity’s water footprint could exceed 30% of current levels. This situation is in stark contrast to the path toward the Sustainable Development Goals, especially Goal 6, “clean water and sanitation”, which also influences Goal 14, “life below water”, and Goal 15, “life on land”. This is because the availability of water directly affects the food security and production capacity of each Country, and therefore its management is a crucial issue worthy of particular attention. Problems related to water security are particularly evident in the Mediterranean area, which is already facing high environmental challenges. It is an area severely affected by global warming; thus, it is one of the most vulnerable environments to climate change globally. It follows that the improper management of water resources could further worsen an already alarming situation. This research aims to study the main water-related challenges that Mediterranean Countries face, highlighting the significant problems that weaken each Country. In this regard, the indicators relating to Goal 6 were considered, to define each Country’s current state. However, for a correct understanding, the main problems these Countries face were researched through a critical review of the literature (Scopus, Google Scholar, Web of Science). In this way, we were able to underline the effects of human activities on the hydrosphere and the repercussions on various ecosystems, following the drivers-pressures-state-impact-response causal framework. The results suggest that there is still a long way for Mediterranean Countries to progress toward Agenda 2030, as they face problems related to chemical (nitrate, microplastics, heavy metals, pesticides, etc.) and biological (E. coli and other microorganisms) pollution, as well as saline aquifers, absent or obsolete infrastructures, and transboundary basins. Hence, this study aims to provide valuable tools for a better evaluation of water management in Mediterranean Countries.

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Bacteroides spp. and traditional fecal indicator bacteria in water quality assessment – An integrated approach for hydric resources management in urban centers

Cited by 23 publications

References 52 publications

Microbial Source Tracking as a Method of Determination of Beach Sand Contamination

Microbial Source Tracking as a Method of Determination of Beach Sand Contamination

Beach Sand Contaminated by Dog Walking? A Molecular Case Study

The Health of the Water Planet: Challenges and Opportunities in the Mediterranean Area. An Overview

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