Bacterium-Sculpted Porphyrin–Protein–Iron Sulfide Clusters for Distinction and Inhibition of Staphylococcus aureus

Abstract

Microbe-catalyzed surface modification is a promising method for the production of special targeting nanomaterials. A bacterium-selective material can be obtained by investigating the microbe-catalyzed mineralization of proteins. Herein, a novel method was fabricated for the biosynthesis of FeS-decorated porphyrin−protein clusters (P-CA@BE) via E. coli (Escherichia coli)catalyzed bio-Fe(III) reduction and bio-sulfidation of porphyrin (P), caffeic acid (CA), and protein [bovine serum albumin (BSA)] assemblies. The assembly (P-CA@BSA) was identified by spectroscopic methods. Next, the P-CA@BSA assembly was transferred into FeS-decorated porphyrin−protein clusters (P-CA@BE) catalyzed by E. coli. There are partial β-folding proteins in P-CA@BE, which selectively recognize S. aureus (Staphylococcus aureus) and show different antibacterial properties against E. coli and S. aureus. Results demonstrate that the E. coli-catalyzed mineralization of the porphyrin−protein assembly is an effective method for the biosynthesis of S. aureus-sensitive metal−protein clusters.

Europium- and Black Phosphorus-Functionalized Porphyrin as an l-Arginine Sensor and l-Arginine-Activated PDT/PTT Agent for Bacterial Treatment

“…Many research reveal that porphyrin tetraanion or tetracation derivatives can recognize compounds with −NH + or −COO – group by luminescence changes. − Inspired by the aforementioned results, we hypothesized the luminescence quench of our BP@Eu-TCPP arised from the strong TCPP conjugation induced by europium complexation and the luminescence can be recovered by interfering with the strong interaction and constructing a weak TCPP conjugation. Therefore, amino acid-activated fluorescence response toward BP@Eu-TCPP was examined.…”

Polylactide—Meso-Substituted Arylporphyrin Composites: Structure, Properties and Antibacterial Activity

“…It can be assumed that the antibacterial properties of porphyrins in the film composites are associated primarily with their effect on the cell walls of microorganisms, by changing their structural properties [ 44 ]. Apparently, the porphyrin molecules in the film composite are capable of disturbing the hydrophilic–lipophilic balance of the cell wall of the contacting microorganism.…”

Photoresponsive Nanoarchitectonics Based on Copper-Porphyrins for Near-Infrared-Enhanced Bacterial Treatment

“…Regarding these issues, the development of photoresponsive compounds for bacterial treatment has garnered significant attention from researchers from different disciplines. , Photodriven therapy, majorly relying on reactive oxygen generated from the reaction of O 2 and photoresponsive compounds, was used as a physio-chemical method for the treatment of drug-resistant bacteria . For this reason, porphyrin derivatives, which were used for photodynamic therapy (PDT) with high singlet oxygen ( 1 O 2 ) generation capacity, have been employed as a powerful near-infrared (NIR)-responsive lipophilic photosensitizer for DNA oxidation and bacterial inhibition. , However, the hypoxic environment and nontargeted damage to normal cells have limited their further application. The functional porphyrin showed both PDT and photothermal (PTT) effects for bacterial treatment .…”

“… 4 For this reason, porphyrin derivatives, which were used for photodynamic therapy (PDT) with high singlet oxygen ( 1 O 2 ) generation capacity, 5 have been employed as a powerful near-infrared (NIR)-responsive lipophilic photosensitizer for DNA oxidation and bacterial inhibition. 6 , 7 However, the hypoxic environment and nontargeted damage to normal cells have limited their further application. The functional porphyrin showed both PDT and photothermal (PTT) effects for bacterial treatment.…”