Bacteriostatic effect of equine pure plateletrich plasma and other blood products against methicillin-sensitive Staphylococcus aureus

López, Catalina; Carmona, JU; Giraldo, Carlos Eduardo; Álvarez, ME

doi:10.3415/vcot-14-04-0054

Cited by 21 publications

(24 citation statements)

References 41 publications

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“…While some beneficial effects such as anti-infectious properties [69], growth factor content [70] and promotion of chemotaxis, proliferation and differentiation of cells [71] have been attributed to leucocytes they also contribute to a greater acute inflammatory response [29, 72] and to the expression of catabolic factors, e.g. MMPs and IL-1, that may influence tendon healing negatively [27, 73, 74], so that a low leucocyte content seems to be preferable to enhance anabolic effects during tendon healing.…”

Section: Discussionmentioning

confidence: 99%

Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies – a randomized prospective controlled clinical trial

et al. 2016

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BackgroundRegenerative and anti-inflammatory effects on tendinopathies have been attributed to blood-derived biologicals. To date the evidence for the efficacy of autologous platelet-rich plasma (PRP) treatment of naturally occurring equine tendinopathies is limited. The purpose of this placebo-controlled clinical trial was to describe the effect of a single treatment of equine superficial digital flexor tendon (SDFT) disease with PRP on clinical and ultrasonographic parameters. Twenty horses with naturally occurring tendinopathies of forelimb SDFTs were randomly assigned to the PRP-treated group (n = 10) or control group (n = 10) after clinical and ultrasonographic examination. The SDFTs received an intralesional treatment with autologous PRP or were injected with saline, respectively (day 0). All horses participated in a standardized exercise programme and were re-examined clinically, with B-mode ultrasonography (5 times at regular intervals) and ultrasound tissue characterization (week 12 and 24 after treatment) until week 24. Long-term performance was estimated via telephone inquiry.ResultsCompared to day 0, lameness decreased significantly by week 8 after treatment with PRP and by week 12 in the control group. Ultrasonographically there was no difference in the summarized cross sectional area between the groups at any time point. Ultrasound tissue characterization showed that echo types representing disorganized matrix decreased significantly throughout the observation period in the PRP-treated group. Echo type II, representing discontinuous fascicles, not yet aligned into lines of stress was significantly higher 24 weeks after PRP treatment. Eighty percent of the PRP treated horses reached their previous or a higher level of performance after 12 months compared to 50 % in the CG. After 24 months these proportions were 60 % and 50 %, respectively.ConclusionsA single intralesional treatment with PRP up to 8 weeks after onset of clinical signs of tendinopathy contributes to an earlier reduction of lameness compared to saline treatment and to an advanced organization of repair tissue as the fibrillar matrix is getting organized into fascicles while remodelling continues. Long term, PRP treatment has the potential to increase the number of horses reaching their previous level of performance. Earlier treatment of tendinopathy with PRP should be considered to enhance these effects.

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Section: Discussionmentioning

confidence: 99%

Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies – a randomized prospective controlled clinical trial

et al. 2016

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“…59 Both TGF-b1 and PDGF-BB contained within PRP have been reported to exert bacteriostatic effects against Staphylococcus aureus. 60 Although several of these molecules are recognized for their chemotactic (CCL3, CCL5, and CXCL1) and immune regulatory activity (TGF-b1), evidence suggests they can also act as antimicrobials upon cleavage. 61,62 Staphylococcus aureus is a common pathogen in infected wounds.…”

Section: Prp Antibacterial Propertiesmentioning

confidence: 99%

Platelet-Rich Plasma for the Treatment of Tissue Infection: Preparation and Clinical Evaluation

Zhang

Guo

Kuss

et al. 2019

Tissue Engineering Part B: Reviews

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The management and treatment of tissue infection, especially chronic infection, represents a significant challenge. Application of autologous platelet-rich plasma (PRP) has emerged as a promising adjunct therapy for facilitating the healing of surgical wounds and tissue injuries. PRP is extracted from whole blood using a sequential centrifugation technique and when activated, can release a vast array of antimicrobial proteins, cytokines, and growth factors. These bioactive molecules are responsible for the ability of PRP to kill pathogens, resolve necrotic tissue, and promote wound healing. PRP is emerging as a useful supplement to prevent postoperative infection and treat chronic wound or bone infections. PRP displays a synergistic effect with antibiotics, which provides unique advantages when treating antibiotic-resistant bacteria. This review will describe the method for PRP preparation and its antibacterial properties, as well as discuss both preclinical in vivo results and evidence from clinical practice of PRP use for the treatment of wound and bone infections.

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“…Some authors have empirically supposed that PCs could promote infections, associating them with microbiological culture media and acting as a substrate for the growth of bacteria ( 27 ). More recently, on the contrary to this theory, some evidence would attribute to PCs an inhibitory effect on bacterial growth, although the results cannot be considered definitive yet ( 27 – 31 ). The antibacterial properties of PCs are supported by some in vitro studies conducted mostly in humans ( 28 , 30 , 32 – 50 ), but also in horses ( 31 , 51 , 52 ) and rabbits ( 53 ).…”

Section: Introductionmentioning

confidence: 90%

“…More recently, on the contrary to this theory, some evidence would attribute to PCs an inhibitory effect on bacterial growth, although the results cannot be considered definitive yet ( 27 – 31 ). The antibacterial properties of PCs are supported by some in vitro studies conducted mostly in humans ( 28 , 30 , 32 – 50 ), but also in horses ( 31 , 51 , 52 ) and rabbits ( 53 ). Systematic reviews of current literature considering in vitro , preclinical, and clinical studies have shown that PCs may have antibacterial properties ( 54 – 56 ).…”

Section: Introductionmentioning

confidence: 90%

Antibacterial Properties of Canine Platelet-Rich Plasma and Other Non-Transfusional Hemo-Components: An in vitro Study

et al. 2021

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This in vitro study was carried out to evaluate the potential antibacterial properties of canine non-transfusional hemo-components. Therapeutic formulations commonly used for regenerative medicine purposes (platelet-rich plasma, platelet gel, platelet lysate, fibrin glue), considering both leukocyte-rich and leukocyte-poor formulations, but also platelet-poor plasma and activating substances (thrombin, calcium gluconate), were tested to detect elements with potential antimicrobial properties. The antibacterial effect was tested on different bacterial strains (Staphylococcus aureus subspecies aureus, Staphylococcus cohnii subspecies cohnii, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae subspecies pneumoniae) isolated from canine wounds and classified as susceptible, multidrug-, extensively, and pandrug-resistant bacteria toward a known panel of human and veterinary antibiotics. The evaluation was carried out by agar gel diffusion method (Kirby–Bauer) and micro-inhibition in broth using microplates and spectrophotometer reading. The study findings confirmed the hypothesized antibacterial properties of canine non-transfusional hemo-components. A more effective bacteriostatic effect was found against Gram-negative bacteria, drug-resistant too. The presence of leukocytes or platelets does not appear to be essential for the antibacterial effect. Further studies should be conducted to evaluate the exact mechanism of action of the antimicrobial activity. However, non-transfusional hemo-components could be a useful natural aid in controlling bacterial infections in dogs.

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Bacteriostatic effect of equine pure plateletrich plasma and other blood products against methicillin-sensitive Staphylococcus aureus

Cited by 21 publications

References 41 publications

Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies – a randomized prospective controlled clinical trial

Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies – a randomized prospective controlled clinical trial

Platelet-Rich Plasma for the Treatment of Tissue Infection: Preparation and Clinical Evaluation

Antibacterial Properties of Canine Platelet-Rich Plasma and Other Non-Transfusional Hemo-Components: An in vitro Study

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