Bacteriostatic antibiotic testing in the Allium test

Kontsevaya, Irina; Zhadko, Svetlana; Alekseenko, O.

doi:10.22281/2686-9713-2020-1-81-88

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…Among higher plants, Allium cepa L. (phylum Angiospermae, class Liliopsida, subclass Lilidae, order Liliales, family Alliaceae, genus Allium L.) has been of interest lately for researchers as a test object for evaluating the genetic effect of chemical compounds in water media (natural and sewage waters, etc.) [12][13].…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic assessment of the quality of media treated with Hermetia Illucens zoocompost components

Porozhnyuk,

Lupandina,

Nepomenko

2024

BIO Web Conf.

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The influence of black soldier fly maggots vermiculture zoocompost water extract components on the proliferative activity of root apexes’ meristem and the root system growth of Allium cepa L was studied. Allium-test was used as a biological analysis, approved by International Program on Chemical Safety (IPCS) for monitoring environmental contaminants, substances, and chemicals. The cytogenetic effects, resulting from the action of zoocompost’s water-soluble components and manifesting themselves in abnormalities of mitosis stages, were identified. The «dose-effect» relationship between the water extract components’ content and the mitotic activity of Allium cepa L meristem was determined. The influence of zoocompost water extract components on the elongation zone of root apexes, which affects root system growth, is demonstrated. It is revealed that the biogenic elements of zoocompost water extract components produce a stimulating effect at certain concentrations. The evaluated doses inhibit the proliferative activity of meristem and the growth of the root system. The inhibiting effect is probably due to the increased salts content in the water extract, which causes plasmolysis of cells’ cytoplasm, and alkalosis, caused by the presence of ammonia. It is recommended to take into account the dosage of biologically active substances in zoocompost during the formulation development of fertilizing mixes for plant cultivation.

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Section: Introductionmentioning

confidence: 99%

Cytogenetic assessment of the quality of media treated with Hermetia Illucens zoocompost components

Porozhnyuk,

Lupandina,

Nepomenko

2024

BIO Web Conf.

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The Tetracyclines Effect on Mitosis’s Pathology in the Root Meristem Allium cepa L. Cells

Kantsavaya

Minina

2022

BSP

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The influence of tetracyclines (tetracycline and doxycycline) on genetic apparatus damage in the cells of root meristem Allium сера L. in the first metaphase after processing and after finishing the recovery period is investigated. Research methods: Allium test, cytogenetic analysis, statistical analysis. The experiment was conducted on a batch of the Stuttgarten variety which is genetically unstable as the result of micronucleus test. It’s indicated that the reaction of the tested material to the effect of tetracyclines depends on the concentration and duration of antibiotics. Aneugenic type aberrations such as mitosis, chromatic agglutination up to the formation of clots were prevailing in the cycling cells. An increase in the proportion of PM (chromatic agglutination, various bridges), the result of which is nucleus pycnosis and cell death was indicated for this genotype after the recovery period. It’s observed that after the recovery period there is a significant increase of cells with mitosis under the influence of tetracycline in the concentration of 20.0 mg/l, on exposure of 23 and 48 hours. The negative influence of tetracycline in the concentration of 100.0 mg/l was investigated, PM value reached 64.2%; the decrease of cells with PM wasn’t observed after the recovery period. In both cases cells with mitosis and chromatic agglutination were dominating. Doxycycline effect in the concentration of 20.0 and 100.0 mg/l caused the doubling of PM in comparison with control. After the recovery period a number of cells regardless of experienced concentration grew due to the increased number of cells with mitosis and chromatic agglutination. It’s necessary to mention different effects of meristematic cells of onion roots under the action of structural isomer which are tetracycline and doxycycline. It’s worth mentioning that the increase in concentration of bacteriostatic antibiotic (tetracycline and doxycycline) is necessary for maximum inactivation on the one hand. On the other hand, negative processes increase in the cells of eukaryotic organisms during the passage of the cell cycle, particularly, mitosis.

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The Effect of Tetracyclines on Mitosis’s Pathology in the Cells of Root Meristem Allium cepa L.

Kantsavaya,

Minina

2022

BSP

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The effect of tetracyclines (tetracycline and doxycycline) on genetic apparatus damage in the cells of root meristem Allium сера L. in the first metaphase after processing and after finishing the recovery period is investigated. Research methods: Allium test, cytogenetic analysis, statistical analysis. The experiment was conducted on a batch of the Stuttgarten variety which is genetically unstable as the result of micronucleus test. It’s indicated that the reaction of the tested material to the effect of tetracyclines depends on the concentration and duration of antibiotics. Aneugenic type aberrations such as mitosis, chromatic agglutination up to the formation of clots were prevailing in the cycling cells. An increase in the proportion of PM (chromatic agglutination, various bridges), the result of which is nucleus pycnosis and cell death was indicated for this genotype after the recovery period. It’s observed that after the recovery period there is a significant increase of cells with mitosis under the influence of tetracycline in the concentration of 20.0 mg/l, on exposure of 23 and 48 hours. The negative influence of tetracycline in the concentration of 100.0 mg/l was investigated, PM value reached 64.2%; the decrease of cells with PM wasn’t observed after the recovery period. In both cases cells with mitosis and chromatic agglutination were dominating. Doxycycline effect in the concentration of 20.0 and 100.0 mg/l caused the doubling of PM in comparison with control. After the recovery period a number of cells regardless of experienced concentration grew due to the increased number of cells with mitosis and chromatic agglutination. It’s necessary to mention different effects of meristematic cells of onion roots under the action of structural isomer which are tetracycline and doxycycline. It’s worth mentioning that the increase in concentration of bacteriostatic antibiotic (tetracycline and doxycycline) is necessary for maximum inactivation on the one hand. On the other hand, negative processes increase in the cells of eukaryotic organisms during the passage of the cell cycle, particularly, mitosis.

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Bacteriostatic antibiotic testing in the Allium test

Cited by 3 publications

References 4 publications

Cytogenetic assessment of the quality of media treated with Hermetia Illucens zoocompost components

Cytogenetic assessment of the quality of media treated with Hermetia Illucens zoocompost components

The Tetracyclines Effect on Mitosis’s Pathology in the Root Meristem Allium cepa L. Cells

The Effect of Tetracyclines on Mitosis’s Pathology in the Cells of Root Meristem Allium cepa L.

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