Bacteriophages inhibit biofilms formed by multi-drug resistant bacteria isolated from septic wounds

Rani, Pallavali Roja; Lakshmi, Degati Vijaya; Praasad, Vijaya Durbaka Vijaya Raghava

doi:10.1101/863076

Cited by 3 publications

(4 citation statements)

References 88 publications

(115 reference statements)

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“… Sutherland et al (2004) reported that phage therapy assumed as an attractive option to prevent and control the formation of biofilm related infections Verma et al (2010) concluded that the biofilms eradication requires the combination of both antibiotics and bacteriophages, while ( Hughes et al, 1998 ) hypothesized that combining bacteriophages with DNase enzymes efficiently destroys the biofilm matrix. However, Pallavali et al (2019) demonstrated that the biofilm may be removed purely by the bacteriophage itself and deemed it a significant development in the hunt for additional agents to replace antibiotics were consistent with the findings of the current investigation. Biofilm makes it difficult for traditional antibiotics to penetrate the cells making them less responsive to antibiotics ( Mittal et al, 2015 ).…”

Section: Discussionsupporting

confidence: 85%

“…These findings were consistent with ( Salim et al, 2013 ). Pallavali et al (2019) suggested that decrease in weight gain due to oxidative stress caused by colibacillosis. Of interest we found that a higher bacteriophage dose and intra-tracheal inoculation lead to significantly decrease shedding of E. coli in lung tissue comparing with infected non treated group ( Tawakol et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Bacteriophage therapy as an alternative biocontrol against emerging multidrug resistant E. coli in broilers

Eid¹,

Tolba

Hamed

et al. 2022

Saudi Journal of Biological Sciences

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Bacteriophage therapy as an alternative biocontrol against emerging multidrug resistant E. coli in broilers

Eid¹,

Tolba

Hamed

et al. 2022

Saudi Journal of Biological Sciences

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“…The phages isolated against K. pneumoniae were also tested for their ability to inhibit and eradicate biofilm production in vitro as previously described. 27 Briefly, freshly grown bacterial culture at OD 600 = 0.5 diluted with L.B broth at factor 10:100 was added to the first column of 96 well micro-titer plates followed by 100 µL of phage suspension at MOI of 0.01 to each well of the first column. 28 The plates were incubated for 8 hours at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Distribution of mcr-1 Harboring Hypervirulent Klebsiella pneumoniae in Clinical Specimens and Lytic Activity of Bacteriophage KpnM Against Isolates

Aslam¹,

Siddique²,

Siddique³

et al. 2022

IDR

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Background The World Health Organization (WHO) has declared the multi-drug resistant (MDR) Klebsiella pneumoniae as one of the critical bacterial pathogens. The dearth of new antibiotics and inadequate therapeutic options necessitate finding alternative options. Bacteriophages are known as enemies of bacteria and are well-recognized to fight MDR pathogens. Methods A total of 150 samples were collected from different clinical specimens through a convenient sampling technique. Isolation, identification, and antibiotic susceptibility testing (AST) of K. pneumoniae were done by standard and validated microbiological procedures. Molecular identification of virulence factors and antibiotic resistance genes (ARGs) was carried out through polymerase chain reaction (PCR) by using specific primers. For bacteriophage isolation, hospital sewage samples were processed for phage enrichment, purification, and further characterization ie, transmission electron microscopy (TEM) and stability testing, etc. followed by evaluation of the lytic potential of the phage. Results Overall, a total of 41% of isolates of K. pneumoniae were observed as hypervirulent K. pneumoniae (hvKp). Among hvKp, a total of 12 (42%) were detected as MDR hvKp. A total of 37% of all MDR isolates were found resistant to colistin, and 66% of the colistin resistance isolates were recorded as mcr-1 positive. Isolated phage KpnM had shown lytic activity against 53 (79%) K. pneumoniae isolates. Remarkably, all 8 mcr-1 harboring MDR hvKp and non-hvKp isolates were susceptible to KpnM phage. Conclusion Significant distribution of mcr-1 harboring hypervirulent Klebsiella pneumoniae was observed in clinical specimens, which is worrisome for the health system of the country. Characterized phage KpnM exhibited encouraging results and showed the lytic activity against the mcr-1 harboring hvKp isolates, which may be used as a prospective alternative control strategy to fight this ominous bacterium.

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“…In addition are combination physiochemical and biological approaches using stains that indicate bacterial viability such as resazurin [ 117 , 118 ] and soluble tetrazolium dyes [ 110 , 119 , 120 , 121 ]. Especially crystal violet staining is used in determining the phage impact on bacterial biofilms, particularly in 96-well microtiter plate formats [ 108 , 122 , 123 , 124 ]. This allows total biofilm biomass quantification [ 125 , 126 ].…”

Section: Improving Phage-biofilm In Vitro Experimentationmentioning

confidence: 99%

Improving Phage-Biofilm In Vitro Experimentation

Abedon

Danis-Wlodarczyk

Wozniak

et al. 2021

Viruses

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Bacteriophages or phages, the viruses of bacteria, are abundant components of most ecosystems, including those where bacteria predominantly occupy biofilm niches. Understanding the phage impact on bacterial biofilms therefore can be crucial toward understanding both phage and bacterial ecology. Here, we take a critical look at the study of bacteriophage interactions with bacterial biofilms as carried out in vitro, since these studies serve as bases of our ecological and therapeutic understanding of phage impacts on biofilms. We suggest that phage-biofilm in vitro experiments often may be improved in terms of both design and interpretation. Specific issues discussed include (a) not distinguishing control of new biofilm growth from removal of existing biofilm, (b) inadequate descriptions of phage titers, (c) artificially small overlying fluid volumes, (d) limited explorations of treatment dosing and duration, (e) only end-point rather than kinetic analyses, (f) importance of distinguishing phage enzymatic from phage bacteriolytic anti-biofilm activities, (g) limitations of biofilm biomass determinations, (h) free-phage interference with viable-count determinations, and (i) importance of experimental conditions. Toward bettering understanding of the ecology of bacteriophage-biofilm interactions, and of phage-mediated biofilm disruption, we discuss here these various issues as well as provide tips toward improving experiments and their reporting.

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Bacteriophages inhibit biofilms formed by multi-drug resistant bacteria isolated from septic wounds

Abstract: 7 8

Cited by 3 publications

References 88 publications

Bacteriophage therapy as an alternative biocontrol against emerging multidrug resistant E. coli in broilers

Bacteriophage therapy as an alternative biocontrol against emerging multidrug resistant E. coli in broilers

Distribution of mcr-1 Harboring Hypervirulent Klebsiella pneumoniae in Clinical Specimens and Lytic Activity of Bacteriophage KpnM Against Isolates

Improving Phage-Biofilm In Vitro Experimentation

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