Bacteriophages in the control of pathogenic vibrios

Plaza, Nicolás; Castillo, Daniel; Pérez-Reytor, Diliana; Higuera, Gastón; García, Katherine; Bastías, Roberto

doi:10.1016/j.ejbt.2017.10.012

Cited by 49 publications

(36 citation statements)

References 138 publications

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“…Despite these limitations, we consider this study as a starting point for further exploration of the ecology and evolution of Vibrio pathogens in aquatic systems. Lysogenic conversion in vibrios could represent a direct concern for human health, food safety and aquaculture industry 34 , 35 , and insight into the influence of phages as carriers of these virulence factors and as vehicles for their dispersal is essential for understanding the role of prophages as drivers of virulence in marine Vibrio communities.…”

Section: Discussionmentioning

confidence: 99%

Widespread distribution of prophage-encoded virulence factors in marine Vibrio communities

Castillo

Kauffman

Hussain

et al. 2018

Sci Rep

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Prophages are known to encode important virulence factors in the human pathogen Vibrio cholerae. However, little is known about the occurrence and composition of prophage-encoded traits in environmental vibrios. A database of 5,674 prophage-like elements constructed from 1,874 Vibrio genome sequences, covering sixty-four species, revealed that prophage-like elements encoding possible properties such as virulence and antibiotic resistance are widely distributed among environmental vibrios, including strains classified as non-pathogenic. Moreover, we found that 45% of Vibrio species harbored a complete prophage-like element belonging to the Inoviridae family, which encode the zonula occludens toxin (Zot) previously described in the V. cholerae. Interestingly, these zot-encoding prophages were found in a variety of Vibrio strains covering both clinical and marine isolates, including strains from deep sea hydrothermal vents and deep subseafloor sediments. In addition, the observation that a spacer from the CRISPR locus in the marine fish pathogen V. anguillarum strain PF7 had 95% sequence identity with a zot gene from the Inoviridae prophage found in V. anguillarum strain PF4, suggests acquired resistance to inoviruses in this species. Altogether, our results contribute to the understanding of the role of prophages as drivers of evolution and virulence in the marine Vibrio bacteria.

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Section: Discussionmentioning

confidence: 99%

Widespread distribution of prophage-encoded virulence factors in marine Vibrio communities

Castillo

Kauffman

Hussain

et al. 2018

Sci Rep

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“…Phage therapy is a re-emerging field, and the bacteriophages represent potential biocontrol agents for the control of virulent and drug-resistant bacteria [19,. However the use of phage therapy in shrimp is still in its early years, and these are highlighted especially the need for using more than one kind of bacteriophage in aquaculture to evade development of bacterial resistance [47][48][49][58][59][60]. Phage therapy has been effectively used to protect against Vibrio diseases in a shrimp and prawn hatchery.…”

Section: Phage Therapy For Vibriosis In Shrimpmentioning

confidence: 99%

Phage Therapy for Control of Bacterial Diseases

Ramasamy¹

2020

Crustacea

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Phage therapy is one of the most important control strategies envisaged for the management of bacterial diseases in the aquatic environment. There are no other effective alternative approaches for the natural control of bacterial diseases, while phage therapy remains the best method which has not yet been exploited. The occurrence, infectivity, lytic activities, therapeutic potentials, and efficacy of the bacteriophages of Bacillus spp./Vibrio spp. for control of pathogenic bacteria diseases such as Vibrio vulnificus, V. damsela, and V. furnissii in the cultures of crustaceans are presented. An ideal method for long-term storage and recovery of the lytic bacteriophages, agar bioassay method and one-step growth experiments, in vivo and in vitro experiments, and validation of the usefulness of phage therapy are described. The review highlights the occurrences of plagues of lytic phages of Vibrio sp. and Bacillus spp. and their control effects of vibriosis both in vivo and in vitro in the crustaceans, thus establishing the application and efficacy of the phages of Vibrio/Bacillus against the pathogenic Vibrio spp. Development of specific phage therapy or a cocktail of phages to a wide variety of systems is considered to represent an interesting emerging alternative to antibiotic therapy and vaccination.

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“…Several Vibrio species are pathogenic, and constitute a serious threat for human health. Over 80 species have been described, and at least 12 of them are known human pathogens [ 27 , 28 ]. V. cholerae , the causative agent of epidemic cholera, was introduced in Europe via sea trade routes from Asia, and was a devastating disease during 1817–1923 [ 29 ].…”

Section: Vibrios In Marine Ecosystemsmentioning

confidence: 99%

“…Although it still remains a serious threat for aquaculture [ 45 , 46 ], a plethora of other Vibrio species have been recorded in the literature as causative agents of vibriosis in aquaculture. V. harveyi , V. parahaemolyticus , V. alginolyticus , V. vulnificus , and V. splendidus [ 28 , 47 , 48 , 49 , 50 , 51 , 52 ] are the most important, while the list is expanding with the discovery of new pathogenic species, such as V. owensii [ 53 ].…”

Section: Vibrios In Aquaculturementioning

confidence: 99%

Bacteriophage Interactions with Marine Pathogenic Vibrios: Implications for Phage Therapy

et al. 2018

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A global distribution in marine, brackish, and freshwater ecosystems, in combination with high abundances and biomass, make vibrios key players in aquatic environments, as well as important pathogens for humans and marine animals. Incidents of Vibrio-associated diseases (vibriosis) in marine aquaculture are being increasingly reported on a global scale, due to the fast growth of the industry over the past few decades years. The administration of antibiotics has been the most commonly applied therapy used to control vibriosis outbreaks, giving rise to concerns about development and spreading of antibiotic-resistant bacteria in the environment. Hence, the idea of using lytic bacteriophages as therapeutic agents against bacterial diseases has been revived during the last years. Bacteriophage therapy constitutes a promising alternative not only for treatment, but also for prevention of vibriosis in aquaculture. However, several scientific and technological challenges still need further investigation before reliable, reproducible treatments with commercial potential are available for the aquaculture industry. The potential and the challenges of phage-based alternatives to antibiotic treatment of vibriosis are addressed in this review.

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Bacteriophages in the control of pathogenic vibrios

Cited by 49 publications

References 138 publications

Widespread distribution of prophage-encoded virulence factors in marine Vibrio communities

Widespread distribution of prophage-encoded virulence factors in marine Vibrio communities

Phage Therapy for Control of Bacterial Diseases

Bacteriophage Interactions with Marine Pathogenic Vibrios: Implications for Phage Therapy

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