Bacteriophages as Potential Tools for Detection and Control of Salmonella spp. in Food Systems

Wei, Shuai; Chelliah, Ramachandran; Oh, Deog‐Hwan; Uddin, Jalal; Ahn, Juhee

doi:10.3390/microorganisms7110570

Cited by 34 publications

(26 citation statements)

References 155 publications

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“…These results are in line with other researchers, who stated that the presence of phages in the farm environment would suggest the bacterial strain has been present at some point in the recent past [12,38]. In addition, this fact could be used for the indirect detection of pathogens based on their specificity towards bacteria [32,39]. In this line, phages against S. Virchow, S. Hadar and S. Ohio were observed to be more prevalent in broilers than in layers.…”

Section: Discussionsupporting

confidence: 91%

“…Phage patterns obtained against different Salmonella serovars per farm in this study indicated that the environment of animal farms, especially poultry operations, could represent an important source of Salmonella phages against several serovars [30,31]. In this sense, the phages obtained could be effective to combat these antibiotic-resistant strains, with the aim of controlling Salmonella AMR and its spread to the food chain [32].…”

Section: Discussionmentioning

confidence: 68%

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Salmonella Bacteriophage Diversity According to Most Prevalent Salmonella Serovars in Layer and Broiler Poultry Farms from Eastern Spain

Sevilla‐Navarro

Catalá-Gregori

Marín

2020

Animals

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The exploration of novel nonantibiotic interventions in the field, such as the use of bacteriophages, is necessary to avoid the presence of Salmonella. Bacteriophages are a group of viruses widely distributed in nature, strictly associated with the prokaryotic cell. Researchers have demonstrated the success of phage therapy in reducing Salmonella counts in poultry products. However, the impact that phage concentration in the environment may have against certain Salmonella serovars is not well understood. Therefore, the aim of this study was to assess Salmonella phage prevalence in commercial poultry farms in terms of the production type: layers or broilers. The most prevalent Salmonella serovars isolated in poultry production were used for phage isolation. Salmonella specific phages were isolated from 141 layer and broiler farms located in the Valencia region during 2019. Analysis of the samples revealed that 100% presented Salmonella phages, the most prevalent being against serovar S. Enteritidis (93%), followed by S. Virchow (59%), S. Typhimurium (55%), S. Infantis (52%) and S. Ohio (51%). These results indicate that poultry farms could represent an important source of Salmonella phages. Nevertheless, further studies are needed to assess the epidemiology of phages against other serovars present in other countries and their diversity from the point of view of molecular studies.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 68%

Salmonella Bacteriophage Diversity According to Most Prevalent Salmonella Serovars in Layer and Broiler Poultry Farms from Eastern Spain

Sevilla‐Navarro

Catalá-Gregori

Marín

2020

Animals

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“…Current methods to treat acute Salmonella gastroenteritis are dependent upon supportive care and antibiotic regimens. The overzealous application of antibiotics, especially in the farm setting, has led to the selection of antimicrobial-resistant strains [17,18], which have contaminated food and water systems, and pose a worldwide threat. Under these circumstances, the measures employed to control Salmonella must not lead antimicrobial resistance, which has garnered interest in biological controls.…”

Section: Introductionmentioning

confidence: 99%

Application of a Broad Range Lytic Phage LPST94 for Biological Control of Salmonella in Foods

et al. 2020

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Salmonella, one of the most common food-borne pathogens, is a significant public health and economic burden worldwide. Lytic phages are viable alternatives to conventional technologies for pathogen biocontrol in food products. In this study, 40 Salmonella phages were isolated from environmentally sourced water samples. We characterized the lytic range against Salmonella and among all isolates, phage LPST94 showed the broadest lytic spectrum and the highest lytic activity. Electron microscopy and genome sequencing indicated that LPST94 belongs to the Ackermannviridae family. Further studies showed this phage is robust, tolerating a wide range of pH (4–12) and temperature (30–60 °C) over 60 min. The efficacy of phage LPST94 as a biological control agent was evaluated in various food products (milk, apple juice, chicken breast, and lettuce) inoculated with non-typhoidal Salmonella species at different temperatures. Interestingly, the anti-Salmonella efficacy of phage LPST94 was greater at 4 °C than 25 °C, although the efficacy varied between different food models. Adding phage LPST94 to Salmonella inoculated milk decreased the Salmonella count by 3 log10 CFU/mL at 4 °C and 0.84 to 2.56 log10 CFU/mL at 25 °C using an MOI of 1000 and 10000, respectively. In apple juice, chicken breast, and lettuce, the Salmonella count was decreased by 3 log10 CFU/mL at both 4 °C and 25 °C after applying phage LPST94 at an MOI of 1000 and 10,000, within a timescale of 48 h. The findings demonstrated that phage LPST94 is a promising candidate for biological control agents against pathogenic Salmonella and has the potential to be applied across different food matrices.

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“…Salmonellosis is one of the most common foodborne diseases, and its control still remains a challenge worldwide (Wei et al, 2019 ). Probiotics have long been used to control Salmonella enteritidis infection (Chambers and Gong, 2011 ; Forkus et al, 2017 ; Gut et al, 2018 ; Lai and Huang, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

The Role of Neurotransmitters in the Protection of Caenorhabditis Elegans for Salmonella Infection by Lactobacillus

Liu

Jiang

et al. 2020

Front. Cell. Infect. Microbiol.

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Salmonellosis is a common foodborne disease. We previously reported the protection of Caenorhabditis elegans from Salmonella Typhimurium DT104 infection by Lactobacillus zeae LB1. However, the mechanism is not fully understood. C. elegans exhibits behavior plasticity when presented with diverse pathogenic or commensal bacteria. Whether it can exert approach avoidance to S . Typhimurium through altering its neurological activity remains to be determined. In the current study, both the wild type and mutants defective in serotonin or dopamine production of C. elegans were used to investigate olfactory preference of the nematode to L. zeae LB1, DT104, and Escherichia coli OP50 by choice assays, and its resistance to DT104 infection and the protection offered by L. zeae LB1 using a life-span assay. The expression of target genes in C. elegans was also examined by real-time quantitative PCR. Results showed that pre-exposure to L. zeae LB1 did not elicit aversive olfactory behavior of the nematode toward DT104. Both mutants tph-1 and cat-2 succumbed faster than the wild type when infected with DT104. While pre-exposure to L. zeae LB1 significantly increased the survival of both the wild type and mutant tph-1 , it provided no protection to mutant cat-2 . Supplementation of dopamine resulted in both the resistance of mutant cat-2 to S . Typhimurium infection and the protection from L. zeae LB1 to the same mutant. Gene expression data also supported the observations in the life-span assay. These results suggest that both serotonin and dopamine play a positive role in the host defense of C. elegans to S . Typhimurium infection and that the L. zeae LB1 protection is not dependent on modifying olfactory preference of the nematode but mediated by dopamine that may have involved the regulation of p38-mitogen-activated protein kinase and insulin/insulin-like growth factor signaling pathways.

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Bacteriophages as Potential Tools for Detection and Control of Salmonella spp. in Food Systems

Cited by 34 publications

References 155 publications

Salmonella Bacteriophage Diversity According to Most Prevalent Salmonella Serovars in Layer and Broiler Poultry Farms from Eastern Spain

Salmonella Bacteriophage Diversity According to Most Prevalent Salmonella Serovars in Layer and Broiler Poultry Farms from Eastern Spain

Application of a Broad Range Lytic Phage LPST94 for Biological Control of Salmonella in Foods

The Role of Neurotransmitters in the Protection of Caenorhabditis Elegans for Salmonella Infection by Lactobacillus

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