Bacteriophages and the control of<i>Erwinia carotovora</i>subsp.<i>carotovora</i>

Ravensdale, Michael; Blom, Theo J.; Gracia-Garza, J.A.; Svircev, A. M.; Smith, R. Scott

doi:10.1080/07060660709507448

Cited by 59 publications

(42 citation statements)

References 34 publications

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“…The potential of phages for the biocontrol of E. amylovora and other plant pathogens (e.g., Pectobacterium carotovora, Xanthomonas campestris pv. vesicatoria, and Agrobacterium tumefaciens) has been demonstrated (4,6,20,21,45,49,53). Balogh et al (5) offer a thorough review of the history, success, and unique considerations for phage therapy in plant agriculture.…”

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confidence: 99%

Novel Virulent and Broad-Host-Range Erwinia amylovora Bacteriophages Reveal a High Degree of Mosaicism and a Relationship to Enterobacteriaceae Phages

Born

Fieseler

Marazzi

et al. 2011

Appl Environ Microbiol

116

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A diverse set of 24 novel phages infecting the fire blight pathogen Erwinia amylovora was isolated from fruit production environments in Switzerland. Based on initial screening, four phages (L1, M7, S6, and Y2) with broad host ranges were selected for detailed characterization and genome sequencing. Phage L1 is a member of the Podoviridae, with a 39.3-kbp genome featuring invariable genome ends with direct terminal repeats. Phage S6, another podovirus, was also found to possess direct terminal repeats but has a larger genome (74.7 kbp), and the virus particle exhibits a complex tail fiber structure. Phages M7 and Y2 both belong to the Myoviridae family and feature long, contractile tails and genomes of 84.7 kbp (M7) and 56.6 kbp (Y2), respectively, with direct terminal repeats. The architecture of all four phage genomes is typical for tailed phages, i.e., organized into function-specific gene clusters. All four phages completely lack genes or functions associated with lysogeny control, which correlates well with their broad host ranges and indicates strictly lytic (virulent) lifestyles without the possibility for host lysogenization. Comparative genomics revealed that M7 is similar to E. amylovora virus ⌽Ea21-4, whereas L1, S6, and Y2 are unrelated to any other E. amylovora phage. Instead, they feature similarities to enterobacterial viruses T7, N4, and ⌽EcoM-GJ1. In a series of laboratory experiments, we provide proof of concept that specific two-phage cocktails offer the potential for biocontrol of the pathogen.

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confidence: 99%

Novel Virulent and Broad-Host-Range Erwinia amylovora Bacteriophages Reveal a High Degree of Mosaicism and a Relationship to Enterobacteriaceae Phages

Born

Fieseler

Marazzi

et al. 2011

Appl Environ Microbiol

116

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“…Several phages infecting Erwinia carotovora subsp. carotovora (14,18) or other Erwinia spp. have been isolated (4,11,12), and a few of these species have been sequenced.…”

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confidence: 99%

“…carotovora) produces numerous plant cell wall-degrading enzymes to cause soft rot diseases in diverse plants (5,6,13,19) and, consequently, causes severe economic losses. Bacteriophages have been considered novel natural biocontrol agents for bacterial plant diseases (8,14). Several phages infecting Erwinia carotovora subsp.…”

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confidence: 99%

Complete Genome Sequence of Pectobacterium carotovorum subsp. carotovorum Bacteriophage My1

Lee

Shin

et al. 2012

J Virol

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c Pectobacterium carotovorum subsp. carotovorum, a member of the Enterobacteriaceae family, is an important plant-pathogenic bacterium causing significant economic losses worldwide. P. carotovorum subsp. carotovorum bacteriophage My1 was isolated from a soil sample. Its genome was completely sequenced and analyzed for the development of an effective biological control agent. Sequence and morphological analyses revealed that phage My1 is a T5-like bacteriophage and belongs to the family Siphoviridae. To date, there is no report of a Pectobacterium-targeting siphovirus genome sequence. Here, we announce the complete genome sequence of phage My1 and report the results of our analysis.

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“…Although phage application after infection had no effect. In another study by Ravensdale et al (2007), Pectobacterium carotovorum load on Calla lily was reduced by phages. The phages however, were inactivated by fertilizer solutions.…”

Section: Phage Therapymentioning

confidence: 99%

Bacteriophage biocontrol of foodborne pathogens

2015

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Bacteriophages are viruses that only infect bacterial cells. Phages are categorized based on the type of their life cycle, the lytic cycle cause lysis of the bacterium with the release of multiple phage particles where as in lysogenic phase the phage DNA is incorporated into the bacterial genome. Lysogeny does not result in lysis of the host. Lytic phages have several potential applications in the food industry as biocontrol agents, biopreservatives and as tools for detecting pathogens. They have also been proposed as alternatives to antibiotics in animal health. Two unique features of phage relevant for food safety are that they are harmless to mammalian cells and high host specificity, keeping the natural microbiota undisturbed. However, the recent approval of bacteriophages as food additives has opened the discussion about 'edible viruses'. This article reviews in detail the application of phages for the control of foodborne pathogens in a process known as Bbiocontrol^.

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Bacteriophages and the control ofErwinia carotovorasubsp.carotovora

Cited by 59 publications

References 34 publications

Novel Virulent and Broad-Host-Range Erwinia amylovora Bacteriophages Reveal a High Degree of Mosaicism and a Relationship to Enterobacteriaceae Phages

Novel Virulent and Broad-Host-Range Erwinia amylovora Bacteriophages Reveal a High Degree of Mosaicism and a Relationship to Enterobacteriaceae Phages

Complete Genome Sequence of Pectobacterium carotovorum subsp. carotovorum Bacteriophage My1

Bacteriophage biocontrol of foodborne pathogens

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