Bacteriophage vB_EcoM_FV3: a new member of “rV5-like viruses”

Truncaitė, Lidija; Šimoliūnas, Eugenijus; Zajančkauskaitė, Aurelija; Kalinienė, Laura; Mankevičiūte, Roma; Staniulis, J.; Klausa, Vytautas; Meškys, Rolandas

doi:10.1007/s00705-012-1449-x

Cited by 15 publications

(16 citation statements)

References 24 publications

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“…Their closest homologue based on BLAST searches is phage vB_EcoM_FFH2, but there are striking differences in the tail fiber genes, and genes encoding DNA modifying enzymes present in vB_EcoM_FFH2, are absent from C203/P206. These genes, as shown by BLAST searches, are rather similar to those found in the Salmonella phages slur12 and slur16 (Smith et al, 2015), the phage EcoM_FV3 (Truncaite et al, 2012) and Murica (Wilder et al, 2015). It has to be noted that the Mauve alignment of rV5-like genomes indicated that the order of genes is generally uniform in rV5 phages, with sequence shifts responsible for different organizations (Figure 2).…”

Section: Discussionsupporting

confidence: 53%

“…In the recent years, rV5-like phages have been established as a 'singleton' genus within the Myoviridae family, showing similar genomic organization and phenotypic characteristics to each other, but exhibiting significant differences to other coliphages (Smith et al, 2015;Truncaite et al, 2012). Based on their morphology, all of them are members of the Myoviridae family, and phage C203 and P206 are no exception from this notion.…”

Section: Discussionmentioning

confidence: 99%

“…rV5-like phages (V5virus genus) are a recently established genus of tailed bacteriophages belonging to the family Myoviridae with a large genome of over 100 kb long (Kropinski et al, 2013;Santos et al, 2011) and exhibiting a relatively wide host spectrum (Kropinski et al, 2013). Several members of the group are notable for their capacity to lyse E. coli O157:H7 strains (Kropinski et al, 2013;Truncaite et al, 2012). In the study which suggested the establishment of the rV5 genus, the authors highlighted the lack of restriction sites, the usage of inner core lipopolysaccharide receptors and the absence of lysogenyassociated genes that contribute to the phages' broad host range and make phage PVP-SE1 good candidate for biocontrol against Salmonella (Santos et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of new broad host-range rV5-like coliphages C203 and P206 directed against enterobacteria

Sváb

Falgenhauer

Rohde

et al. 2018

Infection, Genetics and Evolution

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We isolated and characterized two novel rV5-like lytic bacteriophages from independently collected food samples. Nucleotide sequence analysis revealed that these phages have linear double-stranded DNA genomes comprising 138,073 bp with 213 CDS and 5 tRNA genes. The two genomes contain completely identical nucleotide sequence, albeit there is a 10,718 bp-long shift in the sequence. The GC content of the phage genomes was 43.7% and they showed high general homology to rV5-like phages. The new phages were termed C203 and P206. The genome of both phages contains a unique ORF that encodes for a putative phage homing endonuclease. The phage produced clear plaques with a burst size of approx. 1000 viral particles and a latent period of 60 min. Morphological investigation indicated that the new phages are members of the family Myoviridae with an approximate head length of 85 nm, tail length of 75 nm, and a head width of 96 nm. C203 and P206 exhibit a broad and uniform host range, which included enterohemorrhagic Escherichia coli strains of serogroup O157, multi drug resistant (MDR) E. coli strains of various sero- and pathotypes, and both Shigella sonnei and S. dysenteriae strains. C203 and P206 both effectively reduced the number of living EHEC O157:H7 Sakai in experimentally inoculated minced meat. The same broad host range, the lack of any virulence related genes, the stability and its short latent period suggest that these newly found phages could be suitable candidates as a bio-control agents against food-borne pathogenic Enterobacteria.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of new broad host-range rV5-like coliphages C203 and P206 directed against enterobacteria

Sváb

Falgenhauer

Rohde

et al. 2018

Infection, Genetics and Evolution

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“…Based on these characteristics, phage isolates were classified under the order Caudovirales and Myoviridae family (27). Moreover, these characteristics were similar to those of T1-7-like E. coli phages (4,36). Given the fact that the Myoviridae family contains doublestranded DNA phages (4), all eight phages were presumptively classified under linear double-stranded DNA phages.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Lytic Bacteriophages Infecting Multidrug-Resistant Shiga Toxigenic Atypical Escherichia coli O177 Strains Isolated From Cattle Feces

Montso

Mlambo

Ateba

2019

Front. Public Health

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The increasing incidence of antibiotic resistance and emergence of virulent bacterial pathogens, coupled with a lack of new effective antibiotics, has reignited interest in the use of lytic bacteriophage therapy. The aim of this study was to characterize lytic Escherichia coli O177-specific bacteriophages isolated from cattle feces to determine their potential application as biocontrol agents. A total of 31 lytic E. coli O177-specific bacteriophages were isolated. A large proportion (71%) of these phage isolates produced large plaques while 29% produced small plaques on 0.3% soft agar. Based on different plaque morphologies and clarity and size of plaques, eight phages were selected for further analyses. Spot test and efficiency of plating (EOP) analyses were performed to determine the host range for selected phages. Phage morphotype and growth were analyzed using transmission electron microscopy and the one-step growth curve method. Phages were also assessed for thermal and pH stability. The spot test revealed that all selected phages were capable of infecting different environmental E. coli strains. However, none of the phages infected American Type Culture Collection (ATCC) and environmental Salmonella strains. Furthermore, EOP analysis (range: 0.1-1.0) showed that phages were capable of infecting a wide range of E. coli isolates. Selected phage isolates had a similar morphotype (an icosahedral head and a contractile tail) and were classified under the order Caudovirales, Myoviridae family. The icosahedral heads ranged from 81.2 to 110.77 nm, while the contractile tails ranged from 115.55 to 132.57 nm in size. The phages were found to be still active after 60 min of incubation at 37 and 40 • C. Incremental levels of pH induced a quadratic response on stability of all phages. The pH optima for all eight phages ranged between 7.6 and 8.0, while at pH 3.0 all phages were inactive. Phage latent period ranged between 15 and 25 min while burst size ranged from 91 to 522 virion particles [plaque-forming unit (PFU)] per infected cell. These results demonstrate that lytic E. coli O177-specific bacteriophages isolated from cattle feces are highly stable and have the capacity to infect different E. coli strains, traits that make them potential biocontrol agents.

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“…70%) of its genes were ORFans [67,68]. Since then five other phages have been reported to be rV5-like: coliphages vB_EcoM-FV3 [69], phAPEC8 [70] and phi92 [59], Cronobacter sakazakii phage vB_CsaM_GAP31 [71] and Salmonella phage PVP-SE1 [39]. To this list we can also add Salmonella phage SSE-121 (JX181824); and, coliphage Delta Y that Andrey Letarov and Alla Golomidova (Winogradsky Institute of Microbiology, RAS, Moscow, Russia), isolated from horse manure, and partially sequenced.…”

Section: Discussionmentioning

confidence: 99%

The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5

Kropinski

Waddell

Meng

et al. 2013

Virol J

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BackgroundBacteriophages (phages) have been used extensively as analytical tools to type bacterial cultures and recently for control of zoonotic foodborne pathogens in foods and in animal reservoirs.MethodsWe examined the host range, morphology, genome and proteome of the lytic E. coli O157 phage rV5, derived from phage V5, which is a member of an Escherichia coli O157:H7 phage typing set.ResultsPhage rV5 is a member of the Myoviridae family possessing an icosahedral head of 91 nm between opposite apices. The extended tail measures 121 x 17 nm and has a sheath of 44 x 20 nm and a 7 nm-wide core in the contracted state. It possesses a 137,947 bp genome (43.6 mol%GC) which encodes 233 ORFs and six tRNAs. Until recently this virus appeared to be phylogenetically isolated with almost 70% of its gene products ORFans. rV5 is closely related to coliphages Delta and vB-EcoM-FY3, and more distantly related to Salmonella phages PVP-SE1 and SSE-121, Cronobacter sakazakii phage vB_CsaM_GAP31, and coliphages phAPEC8 and phi92. A complete shotgun proteomic analysis was carried out on rV5, extending what had been gleaned from the genomic analyses. Host range studies revealed that rV5 is active against several other E. coli.

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Bacteriophage vB_EcoM_FV3: a new member of “rV5-like viruses”

Cited by 15 publications

References 24 publications

Identification and characterization of new broad host-range rV5-like coliphages C203 and P206 directed against enterobacteria

Identification and characterization of new broad host-range rV5-like coliphages C203 and P206 directed against enterobacteria

Characterization of Lytic Bacteriophages Infecting Multidrug-Resistant Shiga Toxigenic Atypical Escherichia coli O177 Strains Isolated From Cattle Feces

The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5

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