Bacteriophage Typing of Staphylococcus Aureus

Locatcher-Khorazo, D.; Gutierrez, Elizabeth

doi:10.1001/archopht.1960.00950020776005

Cited by 27 publications

(2 citation statements)

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“…Hence, epidemiological studies of these bacteria are significantly important to determine their source and to control their spread in the community and hospital settings. Different methods such as bacteriophage typing, antibiotyping, genotyping, spa typing-based PCR, and DNA sequencing have been used to investigate the hospital and community-onset S. aureus infections (Locatcher-Khorazo and Gutierrez 1960;Frénay et al 1996;van Leeuwen et al 1999;Yadav et al 2018). Spa typing is based on the polymorphism of the gene encoding protein A (spa).…”

Section: Introductionmentioning

confidence: 99%

Spa typing of Staphylococcus aureus Isolated from Clinical Specimens from Outpatients in Iraq

Abdulkareem

Alzaalan

Yaqoob

2021

Polish Journal of Microbiology

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Methicillin-resistant Staphylococcus aureus (MRSA) is notorious as a hospital superbug and a problematic pathogen among communities. The incidence of MRSA has substantially increased over time in Iraq. The aim of this study was to determine the prevalence and spa types of MRSA isolates from outpatients or patients upon admission into hospitals. Various biochemical tests identified S. aureus isolates, and then this identification was confirmed by PCR using species-specific 16S rRNA primer pairs. Antibiotic susceptibility was determined against methicillin, oxacillin, and vancomycin using the disk diffusion method. Vancomycin MIC was detected by VITEK 2 compact system. All the identified isolates were screened for the presence of mecA and lukS-PV-lukF-PV genes; 36 of them were subjected to spa typing-based PCR. Out of 290 clinical samples, 65 (22.4%) were S. aureus, of which 62 (95.4%) strains were resistant to oxacillin and methicillin. Except for two isolates, all MRSA isolates were mecA positive. One of the three MSSA isolates was mecA positive. Five strains were resistant to vancomycin. Fourteen (21.5%) isolates were positive for the presence of lukS-PV-lukF-PV genes. Spa typing of 36 S. aureus isolates revealed eleven different spa types, t304 (30.3%), t307 (19.4%), t346 (8.3%), t044 (8.3%), t15595 (8.3%), t386 (5.5%), t5475 (5.5%), t17928 (2.8%), t14870 (2.8%), t021 (2.8%), and t024 (2.8%). These findings could be useful for assessing the genetic relatedness of strains in the region for epidemiological and monitoring purposes, which would be essential to limiting the spread of MRSA.

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Section: Introductionmentioning

confidence: 99%

Spa typing of Staphylococcus aureus Isolated from Clinical Specimens from Outpatients in Iraq

Abdulkareem

Alzaalan

Yaqoob

2021

Polish Journal of Microbiology

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“…The anterior chamber is frequently contaminated by bacteria after cataract surgery (Dickey et al 1991, Ariyasu et al 1993, Manners et al 1995, Samad et al 1995, but the fact that endophthalmitis is very rare indicates that a small inoculum of these bacteria can be eradicated by inborn bactericidal properties (McManaway et al 1991). Major risk factors for BE are preoperative external infections (Locatcher-Khorazo & Gutierrez 1960, use of IOL's (Manikoff et al 1991, Vafidis et al 1984, peroperative complications with vitreous loss (Beyer et al 1984, Javitt et al 1991, Speaker & Menikoff 1991, Manikoff et al 1991, and in-patient surgery (Javitt et al 1994).…”

Section: Discussionmentioning

confidence: 99%