Bacteriophage Typing of Proteus mirabilis, Proteus vulgaris, and Proteus morganii

Schmidt, William C.; Jeffries, C. D.

doi:10.1128/aem.27.1.47-53.1974

Cited by 19 publications

(3 citation statements)

References 11 publications

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“…The biotyping scheme of Rauss and Voros (1959) permitted division into only 12 biotypes but, regrettably, most strains (83%) belonged to one biotype. The phage typing scheme of Schmidt and Jeffries (1974) was no more discriminatory. Much better strain discrimination is given either by 0 and H serotyping (Rauss and Voros, 1959) or by bacteriocin typing (Senior, !1987).…”

Section: Analysis Of Strains By Morganocin Sensitivitymentioning

confidence: 90%

Discovery of new morganocin types of Morganella morganii in strains of diverse serotype and the apparent independence of bacteriocin type from serotype of strains

Senior

Vöuröus²

1989

Journal of Medical Microbiology

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A collection of 45 serologically distinct strains of Morganella morganii from several European countries was typed by morganocin production (p) and sensitivity (s). This permitted their differentiation into 33 morganocin p/s types; 15 new types of morganocin and one new morganocin sensitivity type were found and are defined. Morganocin production and sensitivity characteristics appeared to be unrelated to the O and H antigens of the strain. The finest strain recognition in M. morganii might be achieved by a combination of bacteriocin and serotyping methods.

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Section: Analysis Of Strains By Morganocin Sensitivitymentioning

confidence: 90%

Discovery of new morganocin types of Morganella morganii in strains of diverse serotype and the apparent independence of bacteriocin type from serotype of strains

Senior

Vöuröus²

1989

Journal of Medical Microbiology

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“…Moreover, phage-based biocontrol strategies to control Escherichia coli, Bacillus cereus, and Campylobacter jejuni were also developed (Atterbury et al, 2003;Viazis et al, 2011;Bandara et al, 2012). To date, no studies have focused on the use of phages to control M. morganii, although only a few studies related to M. morganii phages have been reported (Schmidt & Jeffries, 1974;Zhu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novelMorganella morganiibacteriophage FSP1 isolated from river water

Yamaki

Omachi

Kawai

et al. 2014

FEMS Microbiol Lett

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Morganella morganii has been identified as a causative agent of opportunistic infections and histamine poisoning. Bacteriophage is a virus and has recently been considered an alternative agent to antibiotics for the control of bacteria that have developed antibiotic resistance. In this study, a novel M. morganii bacteriophage isolated from river water was characterized. The isolated phage, termed FSP1, was purified by polyethylene glycol precipitation followed by cesium chloride density-gradient centrifugation. FSP1 has infectivity against only M. morganii and was identified as a Myoviridae bacteriophage through morphological analysis with transmission electron microscopy. According to the one-step growth curve, the FSP1 latent period, eclipse period, and burst size were 30, 20 min, and 42 PFU infected cell(-1) , respectively. The genome size of FSP1 was estimated to be c. 45.6-49.4 kb by restriction endonuclease analyses. Moreover, challenge testing against M. morganii in vitro revealed that FSP1 had high lytic activity and that the viable cell count of M. morganii was reduced by 6.12 log CFU mL(-1) after inoculation with FSP1 at a multiplicity of infection (MOI) = 10. These results suggested that FSP1 could be used as a biocontrol agent against M. morganii for treatment of infectious disease treatment or food decontamination.

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“…However, numerous typing methods have been proposed to differentiate P. mirabilis. These methods include serological typing (44), the Dienes reaction (20), antimicrobial susceptibility testing (2,13,14), biotyping (2, 13,14,37), resistotyping (35), bacteriophage typing (32,47), and bacteriocin typing (3,18,48). Although bacteriocin typing is an effective method for differentiating strains of Pseudomonas (8,11,26,29), Serratia (25,56,57), and Klebsiella (12,31), the use of bacteriocin typing as an epidemiological tool for P. mirabilis has not yet been adequately investigated in this country.…”

mentioning

confidence: 99%

Typing of Proteus mirabilis by Bacteriocin Production and Sensitivity as a Possible Epidemiological Marker

Kusek

Herman

1980

J Clin Microbiol

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Two bacteriocin typing methods, based on bacteriocin production and bacteriocin sensitivity, were developed to aid in the separation of strains of Proteus mirabilis. One hundred sixty-two isolates of P. mirabilis and 10 Cradock-Watson bacteriocin producers were grown in proteose peptone no. 3 broth and induced with mitomycin C under culture conditions found optimum for bacteriocin production. Crude bacteriocin lysates were spotted on 186 indicator strains, and after incubation for 18 h at 35°C, positive zones of inhibition were recorded. A cluster analysis computer program was used to select 16 bacteriocin-producing and 16 indicator strains for inclusion in two bacteriocin typing sets. One hundred clinical isolates of P. mirabilis were differentiated by bacteriocin sensitivity into 41 distinct patterns, with 72% of the strains typable, whereas typing by bacteriocin production demonstrated 29 separate lysis patterns among the 80% typable strains. Combining the results of each typing method resulted in 72 individual bacteriocin production-sensitivity patterns and 91% of the isolates typed. Typing 14 epidemic strains by bacteriocin production revealed that 13 of 14 strains were identical, whereas only 2 of 14 strains were typable by bacteriocin sensitivity. Electron microscopy of partially purified bacteriocin revealed tail components of contractile bacteriophages. Standard bacteriocin lysates were destroyed by boiling for 1 h. In addition, ail bacteriocin lysates tested were resistant to trypsin. The use of our bacteriocin production typing method against the presently selected 16 standard indicator strains is recommended for the investigation of any cases of suspected P. mirabilis cross-infections within hospitals.

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Bacteriophage Typing of Proteus mirabilis, Proteus vulgaris, and Proteus morganii

Cited by 19 publications

References 11 publications

Discovery of new morganocin types of Morganella morganii in strains of diverse serotype and the apparent independence of bacteriocin type from serotype of strains

Discovery of new morganocin types of Morganella morganii in strains of diverse serotype and the apparent independence of bacteriocin type from serotype of strains

Characterization of a novelMorganella morganiibacteriophage FSP1 isolated from river water

Typing of Proteus mirabilis by Bacteriocin Production and Sensitivity as a Possible Epidemiological Marker

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