Bacteriophage Typing of Listeria monocytogenes Cultures Isolated From Seafoods

Estela, Luis A.; Sofos, John N.; Flores, Barbara B.

doi:10.4315/0362-028x-55.1.13

Cited by 17 publications

(13 citation statements)

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“…Interestingly, two temperate myoviruses of Listeria have been described, namely B054 30-33 and 01761. 34 Both feature genes responsible for host genome integration and maintenance of a temperate lifestyle (ref. 33 and unpublished data).…”

Section: Listeria Phage Genomics and Implications For Phage Evolutionmentioning

confidence: 99%

Listeriaphages

Klumpp

Loessner

2013

Bacteriophage

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is an important foodborne pathogen and the causative agent of Listeriosis, a potentially fatal infection. Several hundred bacteriophages have been described over the past decades, but only few have actually been characterized in some detail, and genome sequences are available for less than twenty of them. We here present an overview of what is currently known about phage genomics, their role in host evolution and pathogenicity, and their various applications in biotechnology and diagnostics.

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Section: Listeria Phage Genomics and Implications For Phage Evolutionmentioning

confidence: 99%

Listeriaphages

Klumpp

Loessner

2013

Bacteriophage

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“…Approximately 320 bacteriophages specific for Listeria (listeriaphages) have been isolated from lysogenic strains or the environment (Andurier et al, 1977;Estela et al, 1992;Loessner, 1991 ;Loessner & Busse, 1990;Ortel & Ackermann, 1985;Rocourt et al, 1982;Sword & Pickett, 1961). Their identification and description largely relies on phage morphology and host ranges.…”

Section: Introductionmentioning

confidence: 99%

“…Listeriaphages are important tools in epidemiological research on the pathogen Listeria monocytogenes (Estela et al, 1992;Loessner & Busse, 1990;McLauchlin et at., 1986;Rocourt et al, 1985), and may be useful in genetic and biotechnology applications. However, surprisingly little information is available on their molecular characteristics (Rocourt et al, 1986;Zink & Loessner, 1992).…”

Section: Introductionmentioning

confidence: 99%

Structural proteins and DNA characteristics of 14 Listeria typing bacteriophages

Loessner¹,

Krause

Henle

et al. 1994

Journal of General Virology

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The major structural proteins of 13 temperate and one virulent Listeria typing bacteriophages were analysed and compared using isoeleetric focusing in immobilized pH gradients (IPG), ultrathin-layer two-dimensional electrophoresis, amino acid analysis and N-terminal amino acid sequences of selected proteins. Isoelectric points for major capsid and tail proteins of the 12 members of the siphoviridae family included in this study ranged from 4.70 to 5.92, whereas one of the two myoviridae investigated (B054) showed structural proteins in the 6.1 to 6.3 range. In comparison to protein profiles from one-dimensional SDS gels, the IPG technique gave better resolution and improved discrimination of phage proteins. Combination of this technique and SDS gel electrophoresis made it possible to correlate M r and isoelectric points of major structural proteins. Tail polypeptides of all siphoviridae are smaller and, with one exception, more acidic than their corresponding capsid counterparts. We also determined the amino acid composition of capsid and tail proteins. When compared with an average protein, they were found to be fairly rich in acidic and short-chain hydrophobic amino acids, as well as in lysine. In addition, the N-terminal amino acid sequences of major capsid and tail proteins of four representative listeriaphages were compared. The base composition of listeriaphage DNAs was between 37 % and 39 % G + C, reflecting that of their bacterial hosts. Each phage had a distinct restriction endonuclease pattern, and genome sizes ranged from 35 to 116 kb. DNA-DNA hybridization permitted the identification of five DNA homology groups. The two myoviruses studied (A511 and B054) showed no DNA homology to other phages, confirming their unique nature. The 12 siphoviruses were classified into three DNA homology groups with little cross-homology. Furthermore, phage A006 was found to share little DNA homology with the other investigated members of species 2671. Therefore, a new species (A006) is proposed. With respect to phage classification and taxonomy, a good correlation between the various approaches was observed, mostly corresponding to particle morphology.

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“…Since the variety of the latter determines the usefulness in a phage-typing sys tem, phages may be selected according to different protein profiles. Therefore, in addition to the 3 listeriaphages already included in a newly described typing scheme (90861,910716, and 93253) [2], a number of these phages (e.g., 01761, B02I, A640. A620, 02971 A, 90666, and PSA) as representatives of the different protein groups should be further tested regarding their value in a com bined, final phage-typing system for the opportunistic pathogen L. monocytogenes.…”

Section: Listeriaphage Protein Compositionsmentioning

confidence: 99%

“…Fifteen of them were isolated in North America from lyso genic strains of L. monocytogenes as described [2], The remaining 5 phages (A005. B021, A640.…”

Section: Isolation Propagation and Purification O F Phagesmentioning

confidence: 99%

Taxonomical Classification of 20 Newly Isolated <i>Listeria</i> Bacteriophages by Electron Microscopy and Protein Analysis

et al. 1994

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A set of 20 newly isolated temperate bacteriophages for phage typing of the pathogen Listeria monocytogenes has been investigated by means of electron microscopy and electrophoretic analysis of phage structural proteins. All phages had isometric capsids (60-64 nm diameter), and long contractile or non-contractile tails of 170-320 nm length. They could be classified into 2 morphotypes (Al and Bl) and were assigned to 3 listeriaphage species (4211, 2671, and 2389). Individual protein profiles were generated by SDS-PAGE of viral polypeptides, as well as isoelectric focusing of solubilized phage proteins in immobilized pH gradient gels. The major structural proteins ranged in size from approximately 15 to 38 kD, and showed isoelectric points from pi 4.3 to 6.2. Protein compositions permitted the differentiation of individual phages as well as the recognition and grouping of similar viruses.

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Bacteriophage Typing of Listeria monocytogenes Cultures Isolated From Seafoods

Cited by 17 publications

References 0 publications

Listeriaphages

Listeriaphages

Structural proteins and DNA characteristics of 14 Listeria typing bacteriophages

Taxonomical Classification of 20 Newly Isolated <i>Listeria</i> Bacteriophages by Electron Microscopy and Protein Analysis

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