Bacteriophage Treatment Reduces Salmonella Colonization of Infected Chickens

Borie, César; Albala, I.; Sánchez, Patricio; Sánchez, Marcelo; Ramírez, Sebastián; Navarro, C.; Morales, Marcelo; Retamales, J.; Robeson, James

doi:10.1637/1933-5334(2008)3[e21:btrsco]2.0.co;2

Cited by 16 publications

(20 citation statements)

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“…For the application of phages in foods, a Salmonella phage, ⌽P7, was tested as a food additive for the control of food-borne Salmonella and produced a significant reduction in Salmonella on a meat surface in a day (7). Furthermore, oral feeding of a Salmonella phage cocktail to broiler chickens as a therapeutic agent showed a rapid reduction in Salmonella colonization in the gut, suggesting that phage therapy may be a good alternative to antibiotic treatment (8).…”

mentioning

confidence: 99%

Characterization and Comparative Genomic Analysis of a Novel Bacteriophage, SFP10, Simultaneously Inhibiting both Salmonella enterica and Escherichia coli O157:H7

Park

Lee

Shin

et al. 2012

Appl Environ Microbiol

154

129

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Salmonella enterica and Escherichia coli O157:H7 are major food-borne pathogens causing serious illness. Phage SFP10, which revealed effective infection of both S. enterica and E. coli O157:H7, was isolated and characterized. SFP10 contains a 158-kb double-stranded DNA genome belonging to the Vi01 phage-like family Myoviridae. In vitro adsorption assays showed that the adsorption constant rates to both Salmonella enterica serovar Typhimurium and E. coli O157:H7 were 2.50 ؋ 10 ؊8 ml/min and 1.91 ؋ 10 ؊8 ml/min, respectively. One-step growth analysis revealed that SFP10 has a shorter latent period (25 min) and a larger burst size (>200 PFU) than ordinary Myoviridae phages, suggesting effective host infection and lytic activity. However, differential development of resistance to SFP10 in S. Typhimurium and E. coli O157:H7 was observed; bacteriophage-insensitive mutant (BIM) frequencies of 1.19 ؋ 10 ؊2 CFU/ml for S. Typhimurium and 4.58 ؋ 10 ؊5 CFU/ml for E. coli O157:H7 were found, indicating that SFP10 should be active and stable for control of E. coli O157:H7 with minimal emergence of SFP10-resistant pathogens but may not be for S. Typhimurium. Specific mutation of rfaL in S. Typhimurium and E. coli O157:H7 revealed the O antigen as an SFP10 receptor for both bacteria. Genome sequence analysis of SFP10 and its comparative analysis with homologous Salmonella Vi01 and Shigella phiSboM-AG3 phages revealed that their tail fiber and tail spike genes share low sequence identity, implying that the genes are major host specificity determinants. This is the first report identifying specific infection and inhibition of Salmonella Typhimurium and E. coli O157:H7 by a single bacteriophage.

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mentioning

confidence: 99%

Characterization and Comparative Genomic Analysis of a Novel Bacteriophage, SFP10, Simultaneously Inhibiting both Salmonella enterica and Escherichia coli O157:H7

Park

Lee

Shin

et al. 2012

Appl Environ Microbiol

154

129

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“…A significant reduction in Salmonella Enteritidis was obtained at that time for both routes of administration with a reduction of more than 1 log 10 CFU/ml in challenged bacterial numbers. This study demonstrates not only that phages are able to reduce Salmonella bacterial loads in broiler chickens but also that aerosol spray and drinking water are conceivable routes of administration in the application of a phage product which will surely facilitate application and establishment of phage biocontrol in an industrial environment (Borie et al, 2008). In a later study these authors, using seven days old chicks were able to replicate the aerosol results.…”

Section: Salmonellamentioning

confidence: 59%

“…(Borie et al, 2008;Borie et al, 2009) chicken Reduction of up to marginal levels in the ileum and six fold in the caeca in the Salmonella counts (Toro et al, 2005) chicken Reduction of up to 6 log 10 in simulated crop environment / 55% in recovery from caecal tonsils of chicks (Andreatti Filho et al, 2007) pig Reduction of up to 3 log 10 reduction of Salmonella colonization (Wall et al, 2010) …”

Section: Target Pathogen Livestock Results Referencesmentioning

confidence: 99%

“…Interestingly, when these resistant bacteria were used to colonize a new group of chickens, they reverted to their phage-sensitive phenotype (Atterbury et al, 2007). Borie et al (2008) pretreated ten day old broiler chicks, by coarse spray or drinking water containing a cocktail of three phages 24 hours before administering roughly 10 6 CFUs of Salmonella Enteritidis (calculated multiplicity of infection (MOI) of 10 3 ). After ten days of infection phages were recovered from the intestinal and other organs.…”

Section: Salmonellamentioning

confidence: 99%

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Phages as Therapeutic Tools to Control Major Foodborne Pathogens: Campylobacter and Salmonella

Carvalho¹,

Santos²,

Kropinski³

et al. 2012

Bacteriophages

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“…Five days post phage treatment, the concentration of S. Enteritidis PT4 per gram of cecal content in the phage-treated group was reduced by 3.5 log units and samples collected up to 25 days after treatment revealed that the treated birds still had lower Colony Forming Units (CFU) of S. Enteritidis PT4 per gram of cecal content compared with untreated broilers. Borie et al (2008) administered a mixture of three phages to chickens 24 h post-infection with Salmonella enteritidis by aerosol spray or oral administration (in drinking water). The results indicated that the phage therapy was effective in reducing S. enteritidis in chickens over a period of 20 days.…”

Section: Control Of Food-borne Pathogens In Poultrymentioning

confidence: 99%

Lytic Bacteriophages as Biocontrol Agents of Foodborne Pathogens

Bhardwaj¹,

Bhardwaj²,

Deep³

et al. 2015

Asian J. of Animal and Veterinary Advances

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The present review focuses on applications of bacteriophages (also called 'Phages') in biocontrol of foodborne pathogens. Food borne diseases caused by bacterial pathogens are a threat to the human health and national economy. Further, there is an increase in multidrug resistance among bacterial pathogens and the conventional methods of food safety, generally involve use of chemicals having certain toxicity issues. The bacteriophages in contrast are natural enemies of bacteria and have regained their popularity as a natural biocontrol agent of bacterial pathogens. The bacteriophages are viruses that kill bacteria and are the most ubiquitous (total number estimated to be 10 30 -10 32 ) known organisms on earth. These are part of the normal microflora of all fresh and unprocessed foods and play a key role in maintaining microbial balance in every ecosystem where bacteria exist. Recently, there is a gaining interest among researchers regarding practical applications of bacteriophages to improve food safety. Many bacteriophage based preparations have been approved by regulatory authorities for their usage in food safety as food additives and disinfectants. Mainly, the application of phages for biocontrol of food pathogens are classified into three categories: (1) Pre-harvest control of foodborne pathogens in food producing livestock and poultry, (2) Decontamination of inanimate surfaces in food-processing facilities and other food establishments and (3) Post-harvest control of foodborne pathogens by direct applications of phages onto the harvested/processed foods. Commercially available phage products being marketed by several companies for reducing the presence of foodborne pathogenic bacteria in food and food production environment have been described and reviewed here.

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Bacteriophage Treatment Reduces Salmonella Colonization of Infected Chickens

Cited by 16 publications

References 0 publications

Characterization and Comparative Genomic Analysis of a Novel Bacteriophage, SFP10, Simultaneously Inhibiting both Salmonella enterica and Escherichia coli O157:H7

Characterization and Comparative Genomic Analysis of a Novel Bacteriophage, SFP10, Simultaneously Inhibiting both Salmonella enterica and Escherichia coli O157:H7

Phages as Therapeutic Tools to Control Major Foodborne Pathogens: Campylobacter and Salmonella

Lytic Bacteriophages as Biocontrol Agents of Foodborne Pathogens

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