Bacteriophage transport through a fining-upwards sedimentary sequence: laboratory experiments and simulation

Flynn, Raymond; Cornaton, Fabien; Hunkeler, Daniel; Rossi, Pierre

doi:10.1016/j.jconhyd.2004.03.001

Cited by 5 publications

(12 citation statements)

References 27 publications

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“…One of the advantages of laboratory-scale studies is that the experimental conditions may be highly controlled, and thus the fundamental processes influencing microorganism transport can be identified (Flynn et al 2004). In particular, column experiments offer the advantage that permission from local authorities is not required, the transport and removal processes can be studied under well-defined physico-chemical conditions, and it has been reported that there are no major advantages of doing batch experiments for viruses as compared to column experiments (Sadeghi et al 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Enteric viruses are shed in extremely high quantities in feces and have very low infectious doses, in the order of tens to hundreds of virions (Borchardt et al 2003;Wong et al 2014). They are frequently isolated from contaminated water and have been linked to numerous waterborne outbreaks (Flynn et al 2004;Zhuang and Jin 2008;Fong et al 2010). Pathogenic viruses have been found in aquifers, groundwater wells, and drinking water (Zhuang and Jin 2003a, b;Weaver et al 2013;Sadeghi et al 2013;Frohnert et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Due to the potential threat to public health, numerous studies have focused on microorganism transport and fate in fractured and porous media (Flynn et al 2004;Masciopinto et al 2008;Cao et al 2010;Chrysikopoulos et al 2010). Theoretical and experimental studies have examined the effect of pore water solution chemistry, fluid velocity, matrix structure, moisture content, temperature, grain size, and presence of surface coatings on viral transport and retention in porous media Chrysikopoulos 2011, 2013;Chrysikopoulos 2006, 2009;Bolster et al 2001;Sim and Chrysikopoulos 2000;Jin et al 1997;Sadeghi et al 2011;Walshe et al 2010;Katzourakis and Chrysikopoulos 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Most viral transport studies focus on bacteriophages, with MS2 being the most common (Powelson et al 1991;Dowd et al 1998;Schijven et al 2002;Weaver et al 2013;Chu et al 2003;Zhuang and Jin 2003a, b;Han et al 2006;Zhuang and Jin 2008;Syngouna and Chrysikopoulos 2011;Wong et al 2014). Other frequently used bacteriophages are: phiX174 (Schijven et al 2002;Kenst et al 2008;Zhuang and Jin 2008;Han et al 2006;Chu et al 2003;Zhuang and Jin 2003a, b;Syngouna and Chrysikopoulos 2011), PRD1 (Schijven et al 2002;Abudalo et al 2005;Foppen et al 2006;Sadeghi et al 2013;Davies et al 2006), and T7 (Flynn et al 2004). Bacteriophages were widely employed as surrogates for human viruses, because they share many properties and features such as size, structure, morphology, and composition.…”

Section: Introductionmentioning

confidence: 99%

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Transport of Human Adenoviruses in Water Saturated Laboratory Columns

et al. 2015

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Groundwater may be contaminated with infective human enteric viruses from various wastewater discharges, sanitary landfills, septic tanks, agricultural practices, and artificial groundwater recharge. Coliphages have been widely used as surrogates of enteric viruses, because they share many fundamental properties and features. Although a large number of studies focusing on various factors (i.e. pore water solution chemistry, fluid velocity, moisture content, temperature, and grain size) that affect biocolloid (bacteria, viruses) transport have been published over the past two decades, little attention has been given toward human adenoviruses (hAdVs). The main objective of this study was to evaluate the effect of pore water velocity on hAdV transport in water saturated laboratory-scale columns packed with glass beads. The effects of pore water velocity on virus transport and retention in porous media was examined at three pore water velocities (0.39, 0.75, and 1.22 cm/min). The results indicated that all estimated average mass recovery values for hAdV were lower than those of coliphages, which were previously reported in the literature by others for experiments conducted under similar experimental conditions. However, no obvious relationship between hAdV mass recovery and water velocity could be established from the experimental results. The collision efficiencies were quantified using the classical colloid filtration theory. Average collision efficiency, α, values decreased with decreasing flow rate, Q, and pore water velocity, U, but no significant effect of U on α was observed. Furthermore, the surface properties of viruses and glass beads were used to construct classical DLVO potential energy profiles. The results revealed that the experimental conditions of this study were unfavorable to deposition and that no aggregation between virus particles is expected to occur. A thorough understanding of the key processes governing virus transport is pivotal for public health protection.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transport of Human Adenoviruses in Water Saturated Laboratory Columns

et al. 2015

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“…A substantial body of research investigating the fundamental processes controlling microbial transport has been carried out at the laboratory scale, often using batch studies and dynamic columns filled with porous media (Mills et al 1994;Bengtsson and Lindqvist 1995;Flynn et al 2004;Franklin and Mills 2005;Foppen et al 2007). Well-established theories exist for explaining and modeling particle transport through homogeneous porous layers (McDowell-Boyer et al 1986;Murphy and Ginn 2000).…”

Section: Introductionmentioning

confidence: 99%

Use of particulate surrogates for assessing microbial mobility in subsurface ecosystems

Sinreich

Flynn²,

Zopfi

2008

Hydrogeol J

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Mass fluxes from the ground surface can play a vital role in influencing groundwater ecosystems. Rates of delivery may influence intact ecosystem composition, while fluxes of substances associated with anthropogenic activity may critically alter the functioning of associated microbial assemblages. Field-based tracing experiments offer a valuable means of understanding mass transport rates and mechanisms, particularly in complex heterogeneous epikarst systems overlying vulnerable fissured aquifers. A short-term tracer experiment monitoring solute and particle tracer concentrations after they passed through a 10-m-thick sequence of limestone, capped by a thin soil, revealed rapid travel times and variable attenuation rates for the substances employed. Results demonstrated that particle tracers have shorter average travel times and can reach the subsurface in higher concentrations and over shorter times than non-reactive solutes. High recovery rates for the bacterial tracer Ralstonia eutropha H16 contrasted strongly with those of similarly sized fluorescent polystyrene microspheres, highlighting the importance of physico-chemical surface characteristics of particle tracers. Complementary laboratory batch experiments examined the role played by organic and inorganic soil/rock surfaces on particle tracer attenuation. Findings suggest that biofilms may significantly promote transport of particulate material below ground, i.e., the delivery of allochthonous microorganisms to karst groundwater.

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Characterizing Aquifer Heterogeneity Using Bacterial and Bacteriophage Tracers

et al. 2015

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Microorganisms constitute an almost exclusive form of life in the earth's subsurface environment (not including caves), particularly at depths exceeding the soil horizon. While of broad interest to ecology and geology, scientific interest in the fate and transport of microorganisms, particularly those introduced through the anthropogenic environment, has focused on understanding the subsurface environment as a pathway for human pathogens and on optimizing the use of microbial organisms for remediation of potable groundwater. This special section, inspired by the 2014 Ninth International Symposium for Subsurface Microbiology, brings together recent efforts to better understand the spatiotemporal occurrence of anthropogenic microbial groundwater contamination and the fate and transport of microbes in the subsurface environment: in soils, deep unsaturated zones, and within aquifer systems. Work includes field reconnaissance, controlled laboratory studies to improve our understanding of specific fate and transport processes, and the development and application of improved mechanistic understanding of microbial fate and transport processes in the subsurface environment. The findings confirm and also challenge the limitations of our current understanding of highly complex microbial fate and transport processes across spatiotemporal scales in the subsurface environment; they also add to the increasing knowledge base to improve our ability to protect drinking water resources and perform in situ environmental remediation.

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Bacteriophage transport through a fining-upwards sedimentary sequence: laboratory experiments and simulation

Cited by 5 publications

References 27 publications

Transport of Human Adenoviruses in Water Saturated Laboratory Columns

Transport of Human Adenoviruses in Water Saturated Laboratory Columns

Use of particulate surrogates for assessing microbial mobility in subsurface ecosystems

Characterizing Aquifer Heterogeneity Using Bacterial and Bacteriophage Tracers

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