Bacteriophage Therapy

Sulakvelidze, Alexander; Alavidze, Zemphira; Morris, J. Glenn

doi:10.1128/aac.45.3.649-659.2001

Cited by 1,326 publications

(1,100 citation statements)

References 28 publications

Supporting

Mentioning

1,053

Contrasting

Unclassified

Order By: Relevance

“…Thus, avoiding requirement for these adjuvants would be a major advantage in clinical applications of DC targeting. In this context, bacteriophage administration has generally proven to be safe [36][37][38], and the results reported here indicate that fd bacteriophages may represent a promising tool also for the formulation of new vaccines.…”

Section: Discussionmentioning

confidence: 73%

Vaccination with filamentous bacteriophages targeting DEC‐205 induces DC maturation and potent anti‐tumor T‐cell responses in the absence of adjuvants

et al. 2011

View full text Add to dashboard Cite

The efficacy of a new vaccine-delivery vector, based on the filamentous bacteriophage fd displaying a single-chain antibody fragment known to bind the mouse DC surface molecule DEC-205, is reported. We demonstrate both in vitro and in vivo an enhanced receptormediated uptake of phage particles expressing the anti-DEC-205 fragment by DCs. We also report that DCs targeted by fd virions in the absence of other stimuli produce IFN-a and IL-6, and acquire a mature phenotype. Moreover, DC-targeting with fd particles double-displaying the anti-DEC-205 fragment on the pIII protein and the OVA [257][258][259][260][261][262][263][264] antigenic determinant on the pVIII protein induced potent inhibition of the growth of the B16-OVA tumor in vivo. This protection was much stronger than other immunization strategies and similar to that induced by adoptively transferred DCs. Since targeting DEC-205 in the absence of DC activation/maturation agents has previously been described to result in tolerance, the ability of fd bacteriophages to induce a strong tumor-specific immune response by targeting DCs through DEC-205 is unexpected, and further validates the potential employment of this safe, versatile and inexpensive delivery system for vaccine formulation.

show abstract

Section: Discussionmentioning

confidence: 73%

Vaccination with filamentous bacteriophages targeting DEC‐205 induces DC maturation and potent anti‐tumor T‐cell responses in the absence of adjuvants

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Bacteriophages have no adverse effects on human or animal immune systems and does not affect the normal bacterial flora [10]. Phages are highly host specific, typically targetting a particular group of bacterial species [11]. Bacteriophages multiply inside the infected host cell in a so called lytic infection cycle, and are released from host cells by bacteriolysis.…”

Section: Introductionmentioning

confidence: 99%

Assessing the impact of bacteriophages in the treatment of Salmonella in broiler chickens

Nabil¹,

Tawakol²,

Hassan³

2018

Infection Ecology & Epidemiology

View full text Add to dashboard Cite

Salmonellosis is one of the main bacterial infections affecting commercial poultry, causing losses to poultry production, and posing a public health concern.Samples from internal organs (liver, cecum and spleen) of one hundred diseased broiler chickens were collected and subjected to Salmonella isolation, identification and serotyping. S. typhimurium and S. enteritidis were selected from the isolated Salmonella to prepare bacteriophages from sewage water taken at broiler farms. An experimental infection of one day old specific pathogen free (SPF) chicks followed by treatment with the prepared bacteriophages isolated from both Salmonella was performed. Caecal samples from infected chicks were subjected at intervals to bacteriophage isolation and Salmonella quantitation. The effectiveness of bacteriophage treatments on Salmonella colonization in cecum of infected chicks increased after five successive doses. At 3 day post infection (dpi), cecal contents showed a marginal decrease in Salmonella loads with more reduction at 5 dpi. From 7 dpi to the end of the experiment at 15 dpi, all the chicks were cleared for both Salmonella.The findings of this study demonstrate that bacteriophage treatment is efficacious in reducing S. typhimurium and S. enteritidis colonization in broiler chickens within a short period and could be used as an alternative to antibiotics.

show abstract

“…Most research has been conducted using viruses that specifically and uniquely search and destroy bacteria. These bacteriophages-'eaters of bacteria'-have been known since the early 20th century [56]. To offer support for the contention that a strategy using mycoviruses in the treatment of medically important fungi can be effective, analogous research using bacteriophages for treating bacterial infections is briefly reviewed.…”

Section: Viruses As Therapeutic Agents For Infections In Humansmentioning

confidence: 99%

“…Although a large number of publications have suggested that bacteriophages may be effective therapeutic agents in clinical settings, most published studies are uncontrolled or anecdotal and do not meet the criteria of modern evidence [56]. However, recently, proof of principle has been provided as bacteriophage therapies have been developed for multiresistant bacteria and other difficult to treat infections [57,58].…”

Section: Viruses As Therapeutic Agents For Infections In Humansmentioning

confidence: 99%