Bacteriophage tail-spike protein derivitized microresonator arrays for specific detection of pathogenic bacteria

Poshtiban, Somayyeh; Singh, Amit; Fitzpatrick, Glen; Evoy, Stéphane

doi:10.1016/j.snb.2012.12.082

Cited by 20 publications

(7 citation statements)

References 29 publications

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“…pneumoniae. It can be used coupled with analytical methods and biosensors, as has been previously reported for other RBPs. ,− ,− Since gp 86 was constructed fused with a fluorescence gene, it can be applied in optical-based diagnostic approaches, such as fluorescence microscopy, ,, enzyme-linked immunoassay (ELISA)-based assays, , or spectrofluorometry …”

Section: Resultsmentioning

confidence: 99%

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Exploitation of a Klebsiella Bacteriophage Receptor-Binding Protein as a Superior Biorecognition Molecule

et al. 2021

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Klebsiella pneumoniae is a Gram-negative bacterium that has become one of the leading causes of life-threatening healthcare-associated infections (HAIs), including pneumonia and sepsis. Moreover, due to its increasingly antibiotic resistance, K. pneumoniae has been declared a global top priority concern. The problem of K. pneumoniae infections is due, in part, to the inability to detect this pathogen rapidly and accurately and thus to treat patients within the early stages of infections. The success in bacterial detection is greatly dictated by the biorecognition molecule used, with the current diagnostic tools relying on expensive probes often lacking specificity and/or sensitivity. (Bacterio)phage receptor-binding proteins (RBPs) are responsible for the recognition and adsorption of phages to specific bacterial host receptors and thus present high potential as biorecognition molecules. In this study, we report the identification and characterization of a novel RBP from the K. pneumoniae phage KpnM6E1 that presents high specificity against the target bacteria and high sensitivity (80%) to recognize K. pneumoniae strains. Moreover, adsorption studies validated the role of gp86 in the attachment to bacterial receptors, as it highly inhibits (86%) phage adsorption to its Klebsiella host. Overall, in this study, we unravel the role and potential of a novel Klebsiella phage RBP as a powerful tool to be used coupled with analytical techniques or biosensing platforms for the diagnosis of K. pneumoniae infections.

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Section: Resultsmentioning

confidence: 99%

“…68 In a similar approach, in the work developed by Poshtiban et al, a glutathione S-transferase (GST) RBP was immobilized in a microresonator-based sensor, which allowed the specific and sensitive detection of C. jejuni. 66…”

Section: Acs Infectious Diseasesmentioning

confidence: 99%

Exploitation of a Klebsiella Bacteriophage Receptor-Binding Protein as a Superior Biorecognition Molecule

et al. 2021

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“…Phage-treated surfaces were not allowed to dry, as drying greatly affects phage binding affinity and lytic properties. 10 For all methods except direct gold adsorption, we saw no measurable desorption of the attached bacteriophages during the washing step. Physical adsorption results in a low surface coverage and dramatically diminishes the sensitivity of the platform.…”

Section: ■ Experimental Sectionmentioning

confidence: 84%

Strategies for the Immobilization of Bacteriophages on Gold Surfaces Monitored by Surface Plasmon Resonance and Surface Morphology

Tawil

Sacher²,

Mandeville

et al. 2013

J. Phys. Chem. C

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The use of bacteriophages as recognition elements for biosensing techniques has recently provoked much interest. Surface plasmon resonance, scanning electron microscopy, and atomic force microscopy were used for the real-time monitoring of the attachment of methicillin-resistant Staphylococcus aureus (MRSA) bacteriophages to gold using several immobilization methods. The MRSA bacterial capture efficiency of phage-functionalized surfaces was studied. We found that whereas the physisorption of phages to gold surfaces affects their biofunctionality, as expressed by their lysing efficiency of bacteria, phages bound via mixed self-assembled monolayers of L-cysteine and 11mercaptoundecanoic acid permitted both the recognition and disruption of bacterial membranes. This is due to the formation of uniform islands on the gold surfaces, permitting an oriented positioning of the phages, thus better exposing their recognition proteins.

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“…Such biosensors typically integrate a bio-recognition probe with a transduction system, as well as electronic systems such as signal amplifiers, processors, and display. Transduction platforms, such as quartz crystal microbalance (QCM) [ 20 , 21 , 22 , 23 ], micromechanical resonators [ 24 , 25 , 26 , 27 , 28 , 29 , 30 ], flow cytometry [ 18 , 31 , 32 , 33 ], amperometry [ 34 , 35 , 36 , 37 , 38 ], surface plasmon resonance (SPR) [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ], and planar optical waveguides [ 47 , 48 , 49 ], have been investigated. In turn, numerous biological probes such as DNA [ 50 ], RNA [ 51 ], monoclonal [ 52 , 53 ] polyclonal antibodies [ 54 ], bacteriophages [ 35 , 55 , 56 , 57 , 58 , 59 ] and their binding proteins [ 58 , 60 ] have been leveraged to impart specificity to the transduction.…”

Section: Introductionmentioning

confidence: 99%

Aryl Diazonium Chemistry for the Surface Functionalization of Glassy Biosensors

et al. 2016

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Nanostring resonator and fiber-optics-based biosensors are of interest as they offer high sensitivity, real-time measurements and the ability to integrate with electronics. However, these devices are somewhat impaired by issues related to surface modification. Both nanostring resonators and photonic sensors employ glassy materials, which are incompatible with electrochemistry. A surface chemistry approach providing strong and stable adhesion to glassy surfaces is thus required. In this work, a diazonium salt induced aryl film grafting process is employed to modify a novel SiCN glassy material. Sandwich rabbit IgG binding assays are performed on the diazonium treated SiCN surfaces. Fluorescently labelled anti-rabbit IgG and anti-rabbit IgG conjugated gold nanoparticles were used as markers to demonstrate the absorption of anti-rabbit IgG and therefore verify the successful grafting of the aryl film. The results of the experiments support the effectiveness of diazonium chemistry for the surface functionalization of SiCN surfaces. This method is applicable to other types of glassy materials and potentially can be expanded to various nanomechanical and optical biosensors.

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Bacteriophage tail-spike protein derivitized microresonator arrays for specific detection of pathogenic bacteria

Cited by 20 publications

References 29 publications

Exploitation of a Klebsiella Bacteriophage Receptor-Binding Protein as a Superior Biorecognition Molecule

Exploitation of a Klebsiella Bacteriophage Receptor-Binding Protein as a Superior Biorecognition Molecule

Strategies for the Immobilization of Bacteriophages on Gold Surfaces Monitored by Surface Plasmon Resonance and Surface Morphology

Aryl Diazonium Chemistry for the Surface Functionalization of Glassy Biosensors

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