Bacteriophage T4 infection mechanisms

Rabussay, Dietmar

doi:10.1016/b978-0-444-80400-6.50013-7

Molecular Aspects of Cellular Regulation

1982

DOI: 10.1016/b978-0-444-80400-6.50013-7

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Bacteriophage T4 infection mechanisms

Dietmar Rabussay¹

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1983

1991

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Cited by 7 publications

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“…The model described above can also accommodate the pleiotropic nature of the ot mutation, if it is assumed that P4 late gene expression requires DNA synthesis, as has been found in P2 and other phage (33). Another possibility, namely, that the polymerase activity is also required for late gene transcription, is rendered less likely by the finding that P4 mRNA synthesis is rifamycin sensitive throughout the life cycle (25).…”

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The bacteriophage P4 alpha gene is the structural gene for bacteriophage P4-induced RNA polymerase

Barrett¹,

Blinkova²,

Arnold³

1983

J Virol

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Two temperature-sensitive mutants of satellite phage P4 which do not synthesize P4 DNA at the nonpermissive temperature have been isolated. One of these phage is mutated in the P4 alpha gene. It complements a P4 delta mutant, but not a P4 alpha amber mutant; both mutants are phenotypically identical to alpha amber mutants in all properties studied. They synthesize P4 early proteins 1 and 2 as well as two additional P4-induced early proteins, 5 and 6, which are described here. P4 late proteins are not synthesized by these mutants and cannot be transactivated by helper phage P2. The mutants are unable to transactivate P2 late proteins from a P2 AB mutant. The P4 RNA polymerase activity which has been suggested to be involved in P4 DNA synthesis is not detected at the nonpermissive temperature. The P4 polymerase activity in partially purified extracts prepared from cells infected with the mutant at the permissive temperature is temperature sensitive. Reduced activity is found in vitro when these extracts are preincubated at 41 degrees C or assayed at temperatures higher than 37 degrees C. Thus, the P4 RNA polymerase is the product of the alpha gene. Temperature shift experiments show that the alpha gene product is required until late in the P4 cycle.

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mentioning

confidence: 99%

The bacteriophage P4 alpha gene is the structural gene for bacteriophage P4-induced RNA polymerase

Barrett¹,

Blinkova²,

Arnold³

1983

J Virol

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Gene product dsbA of bacteriophage T4 binds to late promoters and enhances late transcription

1991

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Gene product 33 of phage T4 is known to be essential in late transcription. Upstream from gene 33 and overlapping its 5' terminal sequence by 20 bp, we identified an open reading frame coding for a binding protein for double-stranded DNA (DsbA). Gene product DsbA is composed of 89 amino acid residues with a Mr of 10376 kDa. We purified this protein to homogeneity from over-expressing cells. Gel retardation assays reveal that it binds to DNA and footprint analyses disclose that it interacts preferentially with T4 late promoter regions. At the sites of binding the protein introduces nicks in double-stranded DNA. In vitro transcription assays performed with T4 late modified RNA polymerase on restriction fragments harbouring a T4 late promoter region prove that gene product DsbA enhances transcription from these promoter regions in the presence of gene product 33. Gene dsbA is distinct from gene das which maps close to this genomic region.

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Changed promoter specificity and antitermination properties displayed in vitro by bacteriophage T4-modified RNA polymerase

Goldfarb

Malik

1984

Journal of Molecular Biology

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Bacteriophage T4 infection mechanisms

Cited by 7 publications

References 523 publications

The bacteriophage P4 alpha gene is the structural gene for bacteriophage P4-induced RNA polymerase

The bacteriophage P4 alpha gene is the structural gene for bacteriophage P4-induced RNA polymerase

Gene product dsbA of bacteriophage T4 binds to late promoters and enhances late transcription

Changed promoter specificity and antitermination properties displayed in vitro by bacteriophage T4-modified RNA polymerase

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