Bacteriophage SPZ7 endolysin: The influence of effectors on the lytic activity of the enzyme on the lysis of gram-negative microorganisms

Klyachko, N. L.; Legotsky, S.A.; Левашов, П. А.; Popova, V. M.; Belogurova, N. G.; Timasheva, A. V.; Dyatlov, I. A.; Levashov, Andrey V.

doi:10.3103/s0027131410030156

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…Ionogenic surfactants have a similar effect on the activity of phos pholipase C, whereas nonionogenic and zwitterionic surfactants do not affect the activity of this enzyme [16]. An enhancement of the activity of endolysin of the bacteriophage in the lysis of Salmonella enteritidis and Escherichia coli cells was observed in the presence of high molecular weight surfactants and pluronics with a large hydrophobic block [17]. Some surfactants have a stabilizing effect on proteins, increasing their thermostability to thermal denaturation [1,18].…”

Section: Introductionmentioning

confidence: 99%

Effect of surfactants of different types on the bacteriolytic activity of lysozyme

et al. 2015

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The influence ofvarious surfactants (anionic sodium dodecyl sulfate, SDS, cationic dodecyltrimethylarnmonium bromide, DTAB, and zwitterionic cocoamidopropylbetaine, CAPB) on the activity of the chicken egg lysozyme is investigated. Lysis of Gram-positive bacteria by the enzyme was carried out at pH 7.2 and ionic strength of 0.15 M. It was found that at low SDS and DTAB concentrations (less than 1 x 10(-5) M) the bacteriolytic activity increases by 30-140%. At higher concentrations (1 x 10(-5) - 1 x 10(4) M) the activity returns to the level observed in the absence of the surfactants. The elevated activity correlated with the formation of hydrophobic lysozyme-surfactant complexes. Introduction of CAPB at concentrations above 1 x 10(-5) M sig, nificantly diminished the bacteriolytic activity due to CAPB induced aggregation of lysozyme.

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Section: Introductionmentioning

confidence: 99%

Effect of surfactants of different types on the bacteriolytic activity of lysozyme

et al. 2015

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Resistance of various soil geosystem bacteria to enzymatic lysis at different pH

Gasanova,

Smirnov,

Rastriga

et al. 2024

E3S Web Conf.

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Bacteria appear to be an inevitable element of soil geochemistry because they participate actively in chemical transformations of soil media components. One of the factors determining the composition of bacterial population in soil is resistance of bacteria to enzymes which hydrolyze cell walls. In the following research, resistance of bacteria (Priestia megaterium, Micrococcus luteus and Escherichia coli) to lysozyme in the pH range appropriate for soils is investigated. All the three species are proved to be most sensitive to lysozyme in slightly alkaline conditions (P. megaterium is least resistant at pH 8.0, M. luteus at pH 8.3, E. coli at pH 8.5), and resistance of all the three microorganisms in the range of pH values of 6.0—7.0 changes insignificantly. A possible factor defining the pH optimum for lysozyme activity in relation to these bacteria is the structure of negatively charged components of their cell walls and outer membranes.

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Bacteriophage SPZ7 endolysin: The influence of effectors on the lytic activity of the enzyme on the lysis of gram-negative microorganisms

Cited by 2 publications

References 18 publications

Effect of surfactants of different types on the bacteriolytic activity of lysozyme

Effect of surfactants of different types on the bacteriolytic activity of lysozyme

Resistance of various soil geosystem bacteria to enzymatic lysis at different pH

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