Bacteriophage Procurement for Therapeutic Purposes

Weber‐Dąbrowska, Beata; Jończyk‐Matysiak, Ewa; Żaczek, Maciej; Łobocka, Małgorzata; Łusiak-Szelachowska, Marzanna; Górski, Andrzej

doi:10.3389/fmicb.2016.01177

Cited by 118 publications

(132 citation statements)

References 137 publications

(178 reference statements)

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“…In this study, four bacteriophages from the Han Cheon River (Anseong, Gyeonggi-do, Korea) proximate to a duck farm were specifically isolated and characterized in terms of their ability to target S. Enteritidis. Several studies have previously documented the isolation of lytic phages against pathogenic Salmonella from different sources such as sewage water (Edham, Al-Tae, & Al-Hammadi, 2017;Łobocka et al, 2014;Sattar, 2018;Weber-Dąbrowska et al, 2016) and fecal samples (Cortés, Spricigo, Bardina, & &Llagostera, 2015), thus highlighting their natural occurrence in the environment. Given that S. Enteritidis is a common pathogen found at Korean poultry farms (Hyeon et al, 2011;Woo, 2005), the isolates of S. Enteritidis bacteriophages from river samples collected proximate to a duck farm.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Salmonella spp.‐specific bacteriophages and their biocontrol application in chicken breast meat

Kim

Jung

et al. 2020

Journal of Food Science

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Chicken breast meat is considered as the main source of Salmonella infection in humans. The aim of this study was to isolate lytic bacteriophages specific for Salmonella enterica serovars Enteritidis and examine their efficacy in a cocktail for the biocontrol of Salmonella spp. in raw chicken breast meat. Four lytic phages belonging to the Myoviridae and Siphoviridae families were isolated from a river proximate to a duck farm. They exhibited broad lytic activities against 11 strains of S. Enteritidis, 11 strains of S. Typhimurium, and one each of S. Paratyphi A, S. San Diego, and S. Typhi. The phages were determined to be stable, exhibited similar degrees of resistance to heat and pH, and had latent periods ranging from 5 to 30 min. In addition, the phage particles were 100% adsorbed within 18 to 40 min. Viable cell counts of bacteria were significantly reduced in raw chicken breast samples (P < 0.05) when treated with a cocktail of all four bacteriophages at 4 °C for 7 days (multiplicities of infection were from 104 to 106). These results indicate the potential efficacy of the bacteriophage cocktail as a biological agent against S. Enteritidis in raw chicken breast meat. Practical Application Our findings demonstrate that the phages could be effective in reducing the viability of Salmonella spp. bacteria in chicken breast meat. Therefore, the phage cocktail is a potential bactericidal agent for the biocontrol of Salmonella spp. in raw chicken breast meat and could be used use in various poultry industries in the future.

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Section: Discussionmentioning

confidence: 99%

Characterization of Salmonella spp.‐specific bacteriophages and their biocontrol application in chicken breast meat

Kim

Jung

et al. 2020

Journal of Food Science

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“…Consequently, interest in the use of bacteriophages (phages) as alternative therapeutic agents for the treatment of infections caused by AMR bacteria has increased in recent years [7]. Successful use of phages for the control of antimicrobial-resistant bacteria has been reported in several contexts, including human medicine [7,10] and the food industry [11]. Unfortunately, the emergence of bacteriophage-insensitive mutants (BIMs) can occur upon repeated exposure of bacterial hosts to specific phage strains [12,13].…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage-Insensitive Mutants of Antimicrobial-Resistant Salmonella Enterica are Altered in their Tetracycline Resistance and Virulence in Caco-2 Intestinal Cells

Fong

Rheault

et al. 2020

IJMS

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Bacteriophages have shown promise as therapeutic alternatives to antibiotics for the control of infectious bacteria, including the human pathogen Salmonella. However, the development of effective phage-based applications requires the elucidation of key interactions between phages and target hosts, particularly since host resistance to phage is inevitable. Little is known about the alteration of host phenotypes following the development of resistance to phage. The aim of this study is to evaluate the antibiotic susceptibility and virulence of a Salmonella isolate following the development of resistance to bacteriophage SI1. We observed enhanced susceptibility to tetracycline and decreased invasion capacity in a differentiated Caco-2 intestinal cell line. Whole genome sequence analysis revealed an array of mutations, most notably, truncations in vgrG1_2, a core gene involved in Type VI secretion and mutations in the lipopolysaccharide, thereby indicating the plausible attachment site of phage SI1. These findings shed light on understanding the underlying mechanism for phage immunity within the host. Importantly, we reveal an associated genetic cost to the bacterial host with developing resistance to phages. Taken together, these results will aid in advancing strategies to delay or eliminate the development of host resistance when designing informed phage-based antimicrobials.

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“…Overcoming phenotypic variations can be solved by combining different phages in a cocktail [145]. This approach permits not only a broader strain-specific range of such preparations, but can also decrease the emergence of resistant bacterial mutants and lead to a faster reduction of the number of bacteria compared to that of individual phage preparations [145,148,149].…”

Section: Individual Phages Versus Cocktailsmentioning

confidence: 99%

Bacteriophages for Chronic Wound Treatment: From Traditional to Novel Delivery Systems

Pinto

Cerqueira

Bañobre-Lópes

et al. 2020

Viruses

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The treatment and management of chronic wounds presents a massive financial burden for global health care systems, with significant and disturbing consequences for the patients affected. These wounds remain challenging to treat, reduce the patients' life quality, and are responsible for a high percentage of limb amputations and many premature deaths. The presence of bacterial biofilms hampers chronic wound therapy due to the high tolerance of biofilm cells to many firstand second-line antibiotics. Due to the appearance of antibiotic-resistant and multidrug-resistant pathogens in these types of wounds, the research for alternative and complementary therapeutic approaches has increased. Bacteriophage (phage) therapy, discovered in the early 1900s, has been revived in the last few decades due to its antibacterial efficacy against antibiotic-resistant clinical isolates. Its use in the treatment of non-healing wounds has shown promising outcomes. In this review, we focus on the societal problems of chronic wounds, describe both the history and ongoing clinical trials of chronic wound-related treatments, and also outline experiments carried out for efficacy evaluation with different phage-host systems using in vitro, ex vivo, and in vivo animal models. We also describe the modern and most recent delivery systems developed for the incorporation of phages for species-targeted antibacterial control while protecting them upon exposure to harsh conditions, increasing the shelf life and facilitating storage of phage-based products. In this review, we also highlight the advances in phage therapy regulation.

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Bacteriophage Procurement for Therapeutic Purposes

Cited by 118 publications

References 137 publications

Characterization of Salmonella spp.‐specific bacteriophages and their biocontrol application in chicken breast meat

Characterization of Salmonella spp.‐specific bacteriophages and their biocontrol application in chicken breast meat

Bacteriophage-Insensitive Mutants of Antimicrobial-Resistant Salmonella Enterica are Altered in their Tetracycline Resistance and Virulence in Caco-2 Intestinal Cells

Bacteriophages for Chronic Wound Treatment: From Traditional to Novel Delivery Systems

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