Bacteriophage P4 DNA replication

Krevolin, Mark D.; Inman, Ross B.; Roof, David M.; Kahn, Michael L.; Calendar, Richard

doi:10.1016/0022-2836(85)90238-4

Cited by 26 publications

(22 citation statements)

References 29 publications

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“…Some of our results on the in vitro P4 replication do not match results reported previously (7,5 (23). The plasmid's replicative helicase RepA unwinds DNA in 5'-3' direction with respect to the strand it has bound.…”

Section: Discussioncontrasting

confidence: 55%

“…Rounds of DNA synthesis initiated at oni proceed to the terminus either bidirectional or alternatively unidirectional. In vivo replicative intermediates originated at ori correspond to the 0-type mode of replication and contain frequently long single-stranded regions at the growing fork, that are deployed in a trans configuration (5). In vivo analysis indicated that P4 replication is independent of DnaA, DnaB, DnaC, and DnaG, but requires DNA polymerase 11 holoenzyme (6).…”

Section: Introductionmentioning

confidence: 99%

“…Such a system has been developed, based on extracts of P4 infected cells and partially purified gpa (5,7). In this system, P4 replication depended strictly on gpca, SSB, and DNA polymerase HI holoenzyme.…”

Section: Introductionmentioning

confidence: 99%

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Phage P4 DNA replicationin vitro

1994

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Phage P4 DNA is replicated in cell-free extracts of Escherichia coli in the presence of partially purified P4 a protein , J. Mol. Biol. 182, [507][508][509][510][511][512][513][514][515][516][517]. Using a modified in vitro replication assay, we have further characterized this process. Analysis by agarose gel electrophoresis and autoradiography of in vitro replicated molecules demonstrates that the system yields supercoiled monomeric DNA as the main product. Electron microscopic analysis of in vitro generated intermediates indicates that DNA synthesis initiates in vitro mainly at ori, the origin of replication used in vivo. Replication proceeds from this origin bidirectionally, resulting in 0-type molecules. In contrast to the in vivo situation, no extensive single-stranded regions were found in these intermediates. The initiation proteins of the host, DnaB and DnaG, and the chaperones DnaJ and DnaK are not required for P4 replication, because polyclonal antibodies against those polypeptides do not inhibit the process. The reaction is inhibited by antibodies against the SSB protein, and by ara-CTP, a specific inhibitor of DNA polymerase Ill holoenzyme. Consistent with previous reports, P4 in vitro replication is independent of transcription by host RNA polymerase. Novobiocin, a DNA gyrase inhibitor, strongly inhibits P4 DNA synthesis, indicating that form I DNA is the required substrate.

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Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Phage P4 DNA replicationin vitro

1994

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“…The helper phage provides the proteins necessary for virion formation, DNA packaging, and hostcell lysis. However, P4 has its own DNA primase, the a gene, which is the only P4 gene known to be absolutely essential for lytic growth (28). Interestingly, the a gene product of retronphage (R73 has the same size as the P4 DNA primase and they share 98% identity (only 14 amino acid substitutions within the 777-residue protein).…”

Section: Resultsmentioning

confidence: 99%

Association of a retroelement with a P4-like cryptic prophage (retronphage phi R73) integrated into the selenocystyl tRNA gene of Escherichia coli

1991

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A new multicopy single-stranded DNA (msDNA-Ec73) was found in a clinical strain of Escherichia coli. consisting The recent discovery of reverse transcriptase (RT) in bacteria raises intriguing questions concerning its origin and functions (22,23,31,34). Although bacterial RTs are highly diverged from each other at the level of primary amino acid sequences (approximately 50o identity) and domain structure, they share conserved sequences with retroviral RTs, indicating that they are evolutionarily related (9,23,52). These bacterial retroelements are required for the synthesis of a peculiar satellite DNA called multicopy single-stranded DNA (msDNA). Bacterial RT genes are found in association with chromosomal regions that encode msDNA, and it has been proposed that this entire primitive retrosystem be termed a retron (49).msDNA consists of a single-stranded DNA ranging in size from 65 to 163 bases, which is branched out from an internal G residue of a short RNA by a 2',5' phosphodiester linkage (see references 24 and 30 for reviews). It is very stable in the cell because of extensive secondary structure in both the DNA and RNA moieties of the msDNA molecule as well as a DNA-RNA hybrid at their 3' ends. msDNA synthesis begins with the production of a long precursor RNA, which folds to form a stable secondary structure such that the folded precursor RNA serves not only as a primer for initiating msDNA synthesis but also as a template to form the branch linkage between the RNA and DNA components.

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“…Replication is unidirectional in P2 (SchnoÈ s and Inman 1971), bidirectional in P4 (Krevolin et al 1985); in P2 it requires the host genes rep , dnaB and dnaG (Bowden et al 1975), but not in P4 (Lindqvist and Six 1971); furthermore, the phage genes involved in the process are unrelated. Lindqvist (1981aLindqvist ( , 1981b succeeded in constructing a hybrid or chimeric phage, P2/P4 Hy19, in which the P4 sequences essential for DNA replication are present together with all P2 sequences required for capsid formation, while all P2 genes known to be needed for P2 DNA replication are a Approximate multiplicities of infection b For crosses 1 and 2, see Materials and methods.…”

Section: Recombination In a P2/p4 Hybrid (Hy19)mentioning

confidence: 97%

Bacteriophage P2: recombination in the superinfection preprophage state and under replication control by phage P4

Bertani

2001

Mol Gen Genomics

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Genetic crosses (mixed infection, lytic cycle) with bacteriophage P2 are known to give extremely low recombination frequencies, and these are unaffected by the recA status of the host bacterium. We now show the following: (1) the satellite bacteriophage P4, which interacts with P2 in a number of ways, but is quite different from it in terms of DNA replication and its control, is clearly dependent on the host recA+ function for recombination; (2) a chimeric phage (Lindqvist's P2/P4 Hy19), in which P2 replication early genes have been replaced by those of P4, recombines in a recA+-dependent manner; (3) immunity-sensitive P2 phages, in mixed infections of P2-immune bacteria, and hence blocked in their replication, recombine in a recA+-dependent manner; (4) an analysis of the distribution of exchanges based on a simple model confirms that in mixed infections of sensitive cells (where P2 is actively multiplying) recombinational exchanges tend to be statistically clustered in a segment of the chromosome containing the origin of replication, and also shows that, under conditions in which P2 DNA replication is blocked, the distribution of exchanges correlates well with the physical distances between markers on the P2 DNA.

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Bacteriophage P4 DNA replication

Cited by 26 publications

References 29 publications

Phage P4 DNA replicationin vitro

Phage P4 DNA replicationin vitro

Association of a retroelement with a P4-like cryptic prophage (retronphage phi R73) integrated into the selenocystyl tRNA gene of Escherichia coli

Bacteriophage P2: recombination in the superinfection preprophage state and under replication control by phage P4

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