Bacteriophage lambda display systems: developments and applications

Nicastro, Jessica; Sheldon, Katlyn; Slavcev, Roderick A.

doi:10.1007/s00253-014-5521-1

Cited by 51 publications

(50 citation statements)

References 93 publications

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“…The gpD protein of the lambda phage is incorporated into the icosahedral phage head with 405–420 copies on a mature phage head as strongly protruding thimble-shaped trivalent spikes. These are promising for multiple tag expression by being attached to different chemical or biological ligands to improve the specificity of cellular uptake, reduce the unwanted immune responses and improve the pharmacokinetics of lambda nanoparticles[87]. Chang at al.…”

Section: Bacteriophages As Nanocarriersmentioning

confidence: 99%

“…Phages can act as gene delivery carriers in gene therapy applications[87, 193–196]. The motivation of utilizing phages for targeted gene delivery is similar to that of applying phages for delivery of DNA vaccines in which the capsid coat preserves the inner DNA from degradation.…”

Section: Applications Of Targeted Phage-based Nanocarriersmentioning

confidence: 99%

“…Artificial covalent conjugation combined with phage display are used to display targeting molecules on the surfaces of phages[199–202]. The use of phages, displaying sequences like fibroblast growth factor (FGF)-derived peptides have been proposed to the target cells having the suitable receptors[87, 122, 203, 204]. Increasing the uptake and endosomal release of phages can be accomplished with protein sequences like the “penton base of adenovirus” which facilitates entry and endosomal release[122, 140, 160, 205].…”

Section: Applications Of Targeted Phage-based Nanocarriersmentioning

confidence: 99%

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Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos

Karimi

Mirshekari

Basri

et al. 2016

Advanced Drug Delivery Reviews

150

108

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The main goal of drug delivery systems is to target therapeutic cargoes to desired cells and to ensure their efficient uptake. Recently a number of studies have focused on designing bio-inspired nanocarriers, such as bacteriophages, and synthetic carriers based on the bacteriophage structure Bacteriophages are viruses that specifically recognize their bacterial hosts. They can replicate only inside their host cell and can act as natural gene carriers. Each type of phage has a particular shape, a different capacity for loading cargo, a specific production time, and their own mechanisms of supramolecular assembly, that have enabled them to act as tunable carriers. New phage-based technologies have led to the construction of different peptide libraries, and recognition abilities provided by novel targeting ligands. Phage hybridization with non-organic compounds introduces new properties to phages and could be a suitable strategy for construction of bio-inorganic carriers. In this review we try to cover the major phage species that have been used in drug and gene delivery systems, and the biological application of phages as novel targeting ligands and targeted therapeutics.

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Section: Bacteriophages As Nanocarriersmentioning

confidence: 99%

Section: Applications Of Targeted Phage-based Nanocarriersmentioning

confidence: 99%

Section: Applications Of Targeted Phage-based Nanocarriersmentioning

confidence: 99%

See 1 more Smart Citation

Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos

Karimi

Mirshekari

Basri

et al. 2016

Advanced Drug Delivery Reviews

150

108

View full text Add to dashboard Cite

show abstract

“…This phenomenon may be related to the host gene disruption by phage integration, CRISPR/Cas system interaction or to mechanisms independent of host background. Another way, to use temperate phages, has been described in detail in a recent review focusing on lambda phage display systems [48]. In that paper, the authors have discussed the advantages and capabilities of phage display utilization as a convenient tool for gene transfer, vaccine design and delivery, as well as bio-sensors, bio-detectors and bio-control agents.…”

Section: Bacteriophagesmentioning

confidence: 99%

Bacteriophages and Phage-Derived Proteins – Application Approaches

Drulis-Kawa

Majkowska-Skrobek²,

Maciejewska³

2015

CMC

170

123

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Currently, the bacterial resistance, especially to most commonly used antibiotics has proved to be a severe therapeutic problem. Nosocomial and community-acquired infections are usually caused by multidrug resistant strains. Therefore, we are forced to develop an alternative or supportive treatment for successful cure of life-threatening infections. The idea of using natural bacterial pathogens such as bacteriophages is already well known. Many papers have been published proving the high antibacterial efficacy of lytic phages tested in animal models as well as in the clinic. Researchers have also investigated the application of non-lytic phages and temperate phages, with promising results. Moreover, the development of molecular biology and novel generation methods of sequencing has opened up new possibilities in the design of engineered phages and recombinant phage-derived proteins. Encouraging performances were noted especially for phage enzymes involved in the first step of viral infection responsible for bacterial envelope degradation, named depolymerases. There are at least five major groups of such enzymes – peptidoglycan hydrolases, endosialidases, endorhamnosidases, alginate lyases and hyaluronate lyases – that have application potential. There is also much interest in proteins encoded by lysis cassette genes (holins, endolysins, spanins) responsible for progeny release during the phage lytic cycle. In this review, we discuss several issues of phage and phage-derived protein application approaches in therapy, diagnostics and biotechnology in general.

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“…The DNA is injected into the host bacteria and is stably integrated into the host chromosome during the lysogenic state. When triggered correctly, the λ phage starts a lytic cycle [38]. …”

Section: Phage Display Systems Used For Proteomic Phage Displaymentioning

confidence: 99%

Interaction Analysis through Proteomic Phage Display

Sundell

Ivarsson

2014

BioMed Research International

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Phage display is a powerful technique for profiling specificities of peptide binding domains. The method is suited for the identification of high-affinity ligands with inhibitor potential when using highly diverse combinatorial peptide phage libraries. Such experiments further provide consensus motifs for genome-wide scanning of ligands of potential biological relevance. A complementary but considerably less explored approach is to display expression products of genomic DNA, cDNA, open reading frames (ORFs), or oligonucleotide libraries designed to encode defined regions of a target proteome on phage particles. One of the main applications of such proteomic libraries has been the elucidation of antibody epitopes. This review is focused on the use of proteomic phage display to uncover protein-protein interactions of potential relevance for cellular function. The method is particularly suited for the discovery of interactions between peptide binding domains and their targets. We discuss the largely unexplored potential of this method in the discovery of domain-motif interactions of potential biological relevance.

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Bacteriophage lambda display systems: developments and applications

Cited by 51 publications

References 93 publications

Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos

Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos

Bacteriophages and Phage-Derived Proteins – Application Approaches

Interaction Analysis through Proteomic Phage Display

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