Bacteriophage isolation from human saliva: a pilot study with high school students

Abstract

View related articlesView Crossmark data increase [1,2]. However, there is still a lack of information for the Portuguese population for this issue. This exploratory study aimed to evaluate the relationship between patient perception of OHRQoL and the severity of dental malocclusion in a Portuguese sample. Materials and methods: This work was approved by the Egas Moniz Ethics Committee. This cross-sectional observational study involved patients that sought orthodontic treatment between January and April 2019, at the Orthodontic Care Consultation of Egas Moniz Dental Clinic (Monte de Caparica -Almada, Portugal). Exclusion criteria were patients with severe diseases, craniofacial abnormalities, cognitive deficits, caries, periodontal diseases, and previous orthodontic treatment history. A total of 19 patients were enrolled in the study. OHRQoL was assessed by application of the Oral Health Impact Profile -Portuguese validated version [3] and dental malocclusion through the Index of Complexity, Outcome and Need (ICON) [4]. Based on the ICON score, patients were categorised as: in need of treatment (ICON > 43) or not (ICON 43). Resulting data were submitted to descriptive and inferential statistical analysis. Results: The sample included 7 (37%) males and 12 (63%) females, with a mean age of 27.9 years. Overall OHIP-14 score ranged from 0 to 49 and ICON score from 13 to 75 (9 (47.4%) subjects with ICON > 43 and 10 (52.6%) with ICON 43). Total OHIP-14 score and all seven median domain scores were not found to be significantly different (p ¼ .113 to p ¼ .968), when comparing both groups. Discussion and conclusions: OHRQoL was not found to be significantly different when considering the severity of dental malocclusion. Overall, results highlight a difference between patients' self-perception of clinical condition impact in OHRQoL when compared to a clinical expertise judgement.

Induced Burkholderia prophages detected from the hemoculture: a biomarker for Burkholderia pseudomallei infection

“…In the human body, phages are found in many parts, including the skin, oral cavity, lungs, gut, and urinary tract ( Barr, 2017 ). E. faecalis phages can be isolated from healthy human saliva with an isolation rate of 9.8% ( Bachrach et al, 2003 ; Nascimento et al, 2021 ). S. epidermidis phages could be isolated from human skin, with an isolation rate of 46% ( Valente et al, 2021 ).…”

SVep1, a temperate phage of human oral commensal Streptococcus vestibularis

“…Similarly, out of 254 test samples (including saliva, teeth, dental plaque, dental effluent, and general sewage), only one lytic phage targeting S. mutans , named SMHBZ8, was identified ( Ben-Zaken et al, 2021 ). Another recent study attempted to isolate lytic phages of S. mutans from the saliva of 60 volunteers but did not succeed ( Nascimento et al, 2021 ). It is plausible that the absence of lytic phages in sewage and the 30 saliva samples used in our studies could be attributed to various factors influencing phage isolation.…”