Bacteriophage-based techniques for elucidating the function of zebrafish gut microbiota

Jia, Pan‐Pan; Yang, Yifan; Junaid, Muhammad; Jia, Huang-Jie; Li, Weiguo; Pei, De‐Sheng

doi:10.1007/s00253-023-12439-x

Cited by 4 publications

(2 citation statements)

References 148 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CRISPR/Cas9 technology is widely integrated in zebrafish studies which can enable the researchers to generate zebrafish lines with precise gene mutations related to inflammation [ [180] , [181] , [182] ]. Moreover, for IBD, various studies are being performed to learn about the interaction between the zebrafish gut microbiome and the immune system [ 178 , 183 , 184 ]. Various in vivo imaging studies can enable real-time visualization of the progress of inflammation and the effects of drugs in suppressing the same.…”

Section: Challenges and Future Prospectivesmentioning

confidence: 99%

A review on current advancement in zebrafish models to study chronic inflammatory diseases and their therapeutic targets

Balde,

Ramya,

Nazeer

2024

Heliyon

View full text Add to dashboard Cite

Section: Challenges and Future Prospectivesmentioning

confidence: 99%

A review on current advancement in zebrafish models to study chronic inflammatory diseases and their therapeutic targets

Balde,

Ramya,

Nazeer

2024

Heliyon

View full text Add to dashboard Cite

“…The extensive usage of antibiotics globally has led to the emergence of multidrug-resistant (MDR) strains in many pathogenic bacteria ( Sun et al, 2022 ; Ul-Hamid et al, 2022 ). Phage therapy, which involves the use of phages that specifically infect and kill the host bacteria without affecting other bacteria, is a promising strategy against MDR ( Rodriguez-Gonzalez et al, 2020 ; Kim et al, 2021 ; Salazar et al, 2021 ; Jia et al, 2023 ). However, the high host specificity and narrow host range of most phages limit their effectiveness for use in phage therapy ( Zhan et al, 2015 ; Jeon et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions

Jia

Yin

et al. 2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Bacteriophages, the most abundant organisms on earth, have the potential to address the rise of multidrug-resistant bacteria resulting from the overuse of antibiotics. However, their high specificity and limited host range can hinder their effectiveness. Phage engineering, through the use of gene editing techniques, offers a means to enhance the host range of bacteria, improve phage efficacy, and facilitate efficient cell-free production of phage drugs. To engineer phages effectively, it is necessary to understand the interaction between phages and host bacteria. Understanding the interaction between the receptor recognition protein of bacteriophages and host receptors can serve as a valuable guide for modifying or replacing these proteins, thereby altering the receptor range of the bacteriophage. Research and development focused on the CRISPR-Cas bacterial immune system against bacteriophage nucleic acids can provide the necessary tools to promote recombination and counter-selection in engineered bacteriophage programs. Additionally, studying the transcription and assembly functions of bacteriophages in host bacteria can facilitate the engineered assembly of bacteriophage genomes in non-host environments. This review highlights a comprehensive summary of phage engineering methods, including in-host and out-of-host engineering, and the use of high-throughput methods to understand their role. The main aim of these techniques is to harness the intricate interactions between bacteriophages and hosts to inform and guide the engineering of bacteriophages, particularly in the context of studying and manipulating the host range of bacteriophages. By employing advanced high-throughput methods to identify specific bacteriophage receptor recognition genes, and subsequently introducing modifications or performing gene swapping through in-host recombination or out-of-host synthesis, it becomes possible to strategically alter the host range of bacteriophages. This capability holds immense significance for leveraging bacteriophages as a promising therapeutic approach against antibiotic-resistant bacteria.

show abstract

Bacteriophage genome engineering for phage therapy to combat bacterial antimicrobial resistance as an alternative to antibiotics

2023

View full text Add to dashboard Cite

Bacteriophage-based techniques for elucidating the function of zebrafish gut microbiota

Cited by 4 publications

References 148 publications

A review on current advancement in zebrafish models to study chronic inflammatory diseases and their therapeutic targets

A review on current advancement in zebrafish models to study chronic inflammatory diseases and their therapeutic targets

Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions

Bacteriophage genome engineering for phage therapy to combat bacterial antimicrobial resistance as an alternative to antibiotics

Contact Info

Product

Resources

About